盐酸普鲁卡因对白血病HL-60细胞增殖与凋亡的影响

目的:探讨盐酸普鲁卡因对白血病HL-60细胞增殖与凋亡的影响.方法:选用HL-60人原髓细胞白血病细胞株,经细胞培养、铺板后,加入不同浓度的盐酸普鲁卡因进行分组,分为0mM(对照组)、1.5mM组、3.0mM组、4.5mM组、6.0mM组、7.5mM 组.采用MTT比色法检测细胞增殖能力,采用流式细胞仪检测细胞凋亡.结果:不同浓度的盐酸普鲁卡因作用24h后,6.0mM组、7.5mM组与对照组比较,对HL-60细胞的抑制率具有统计学意义(P<0.05);作用48h后,3.0mM组、4.5mM组、6.0mM组、7.5mM组与对照组比较,对HL-60细胞的抑制率具有显著性统计学意义(P<0.01);作用72h后,1.5mM组、3.0mM组、4.5mM组、6.0mM组、7.5mM组与对照组比较,对HL-60细胞的抑制率具有显著性统计学意义(P<0.01).流式细胞术检测HL-60细胞凋亡,结果显示,按照0mM、2mM、4mM、6mM的顺序,表现出的诱导细胞凋亡逐渐增强,6mM时诱导作用达到最强.0mM、2mM、4mM、6mM培养HL-60细胞24h后,HL-60细胞的凋亡率分别为(2.50±0.53)%、(2.83±0.75)%、(9.22±1.65)%和(17.36±3.81)%,无论早期凋亡、晚期凋亡,还是凋亡总和,与0mM、2mM相比,4mM、6mM时的细胞凋亡显著增强,具有显著性统计学差异(P<0.01).结论:盐酸普鲁卡因对白血病HL-60细胞增殖具有抑制作用,且可诱导细胞凋亡,且可能具有一定的时间-剂量正相关效应,这也为白血病的临床诊疗、机制阐释等提供了新的方向和思路.

Production of Gel with Menthol, Benzocaine and Procaine HCl for Topical Application in Rheumatoid Arthritis Patients

Antirheurnatic and analgesics drugs are the most commonly prescribed medications for treatment of acute and chronic pain. Frequent application of these agents often causes adverse effects. So, in order to avoid the incidence of side effects, topical analgesics are treatment of choice for localized muscle pain. Topical analgesics offer the potential to provide the same analgesic relief provided by oral analgesics but with minimal adverse systemic effects. The aim of this study is to present the preparation and characterization of topical analgesic gel named ＂Russian water＂. Gel is composed of Menthol, Benzocaine, Procaine HCl as topical analgesic agents indicated for temporary relief of pain. Obtained results showed prolonged analgesic effect compared with liquid ethanol solution composed of the same components.