Monitoring the stability of steep slopes of open-pit mines is a major issue relating to production safety in mines.In order to determine the technical parameters of a new type of supervising system applied in monitori...Monitoring the stability of steep slopes of open-pit mines is a major issue relating to production safety in mines.In order to determine the technical parameters of a new type of supervising system applied in monitoring steep slopes of open-pit mines,the MSARMA method was used to establish analytical models for the monitoring system,given various parameter settings based on the description of mechanical monitoring principles.We used this sensitivity analysis to conclude that the setting of the most sensitive location of a mechanical monitoring system should be within a range of 1/5～1/2 of the lower part in a vertical direction of steep slopes,with a rational and feasible range of the dip angle setting between 0°～20°.Given the analytical results of our on-site experiments,we have shown that the parameters determined reflect the stability of steep slopes accurately and effectively.These conclusions provide a basis for the application of a new type of steep slope stability monitoring technology in open-pit mines.展开更多
In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly lucife...In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR,from-7 to 1012).The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR.One clone,designated BFVL,was selected from ten neomycin-resistant clones.BFVL showed a specific and inducible dose-and time-dependent luciferase activity in response to BFV infection.Although the changes in luciferase activity of BFVL peaked at 84 h post infection,it was possible to differentiate infected and uninfected cells at 48 h post infection.A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL.Moreover,the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection.These findings suggest that the BFVL-based assay is rapid,easy,sensitive,quantitative and specific for detection of BFV infection.展开更多
基金Project 1053G032 supported by the Youth Science Foundation of Educational Committee of Heilongjiang Province
文摘Monitoring the stability of steep slopes of open-pit mines is a major issue relating to production safety in mines.In order to determine the technical parameters of a new type of supervising system applied in monitoring steep slopes of open-pit mines,the MSARMA method was used to establish analytical models for the monitoring system,given various parameter settings based on the description of mechanical monitoring principles.We used this sensitivity analysis to conclude that the setting of the most sensitive location of a mechanical monitoring system should be within a range of 1/5～1/2 of the lower part in a vertical direction of steep slopes,with a rational and feasible range of the dip angle setting between 0°～20°.Given the analytical results of our on-site experiments,we have shown that the parameters determined reflect the stability of steep slopes accurately and effectively.These conclusions provide a basis for the application of a new type of steep slope stability monitoring technology in open-pit mines.
基金National Natural Science Foundation of China(31070135,81071343)
文摘In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR,from-7 to 1012).The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR.One clone,designated BFVL,was selected from ten neomycin-resistant clones.BFVL showed a specific and inducible dose-and time-dependent luciferase activity in response to BFV infection.Although the changes in luciferase activity of BFVL peaked at 84 h post infection,it was possible to differentiate infected and uninfected cells at 48 h post infection.A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL.Moreover,the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection.These findings suggest that the BFVL-based assay is rapid,easy,sensitive,quantitative and specific for detection of BFV infection.
