盘花垂头菊倍半萜和酚类化合物结构的研究

从盘花垂头菊全草分离出 10个化合物 ,经 IR,1H NMR,13 C NMR,DEPT,FABMS,EIMS等光谱方法分别鉴定为 :1β ,8-二当归酰氧基 - 2β -乙酰氧基 - 3β -羟基 - 4α -氯 - 10 ,11-环氧 -没药 - 7(14) -烯 (1) ;化合物 1的异构体 (2 ) ,对羟基苯甲酸 ,2 - (2′-正十九酰基 ) -苯乙基酯 (3a) ;对羟基苯甲酸 ,2 - (2′-正二十一酰基 ) -苯乙基酯 (3b) ;对羟基苯甲醛 (4) ;对羟基苯乙酮 (5 ) ;苯甲酸 (6 ) ;4 -羟基 - 3,5 -二甲氧基苯甲醛 (又称丁香醛 ) (7) ;7-甲氧基 - 6 -羟基香豆素 (又称异东莨菪素 ) (8) ;乌苏酸 (9) .其中 1,2 ,3a和 3b是新化合物 ,其它化合物 (4～ 9)是首次从该属植物中分到的 .

盘花垂头菊中两种新型倍半萜类化合物抑制人肝癌SMMC-7721细胞生长

AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.

高山植物盘花垂头菊和唐古特乌头光合膜系统的超微结构研究

对生长在青藏高原东北部海拔高度为3700m处的盆花垂头（Cremanthodiumdiscoideum）和唐古特乌头（Aconitumtangutcum）叶肉细胞叶绿体光合膜系进行超微分析表明：叶绿体被膜异常，基拉片层叠垛程度小，基粒和基质类囊体肿胀严重。叶绿体超微结构上的这些特征，是青藏高原低气压、严寒和强辐射等生态条件综合作用的结果。

盘花垂头菊中两种新型倍半萜类化合物诱导人肝癌细胞SMMC-7721凋亡

[目的]为开发新型肿瘤治疗药物提供依据。[方法]通过单细胞电泳和流式细胞分析法等技术,研究2种从盘花垂头菊中分离的高含氧甜没药烯型倍半萜化合物(HOBS)对人肝癌细胞SMMC-7721的抑制作用。[结果]2种HOBS处理48 h后,SMMC-7721细胞体积变小,且出现膜泡化和凋亡小体。单细胞电泳试验表明SMMC-7721细胞经不同浓度HOBS处理后,DNA碎片在电场中呈彗星状向阳极移动,且彗星样尾迹随药物浓度的增大而增长。HOBS能以剂量依赖的方式诱导SMMC-7721细胞凋亡。当2种HOBS浓度为8μg/ml时,细胞凋亡率分别达36.7%和38.9%。凋亡率随药物浓度的增大而升高,与对照组相比有显著差异,并出现典型的凋亡峰。2种HOB可使细胞内[Ca2+]i水平升高。[结论]HOBS能诱导SMMC-7721细胞凋亡。