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一种简单高效的聚丙烯酰胺凝胶DNA回收方法 被引量:5
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作者 漆雪琳 李博 +2 位作者 宋顺 陈新 彭明 《中国农学通报》 CSCD 北大核心 2011年第18期214-217,共4页
笔者介绍了一种简单的聚丙烯酰胺凝胶DNA回收方法(直取法),通过与传统煮沸法比较以证明直取法回收聚丙烯酰胺凝胶DNA的高效性。以木薯cDNA为材料,将直取法与常规煮沸法从实验方法、杂带污染率以及回收扩增率3个方面进行比较。实验结果表... 笔者介绍了一种简单的聚丙烯酰胺凝胶DNA回收方法(直取法),通过与传统煮沸法比较以证明直取法回收聚丙烯酰胺凝胶DNA的高效性。以木薯cDNA为材料,将直取法与常规煮沸法从实验方法、杂带污染率以及回收扩增率3个方面进行比较。实验结果表明:直取法较煮沸法简单、快速;煮沸法的杂带污染率为59.09%,直取法为43.08%;煮沸法的平均产物量约为40ng/μL,直取法约为60ng/μL。研究结果表明,直取法相对煮沸法简单,快速,扩增效率高,杂带污染几率小。 展开更多
关键词 聚丙烯酰胺凝胶电泳 DNA回收 煮沸法 直取法 木薯
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马骡捶结术及其在兽医临床上应用
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作者 施进文 武金利 《中国兽医杂志》 CAS 北大核心 2003年第7期24-25,共2页
结症又称肠阻塞,是马骡胃肠运化机能减退粪便积滞于某一肠段,引起肠道阻塞不通的一种腹痛起卧疾病.结症的发生与饲养管理不善及使役不当等因素有密切关系.
关键词 肠阻塞 捶结术 兽医 临床医学 应用 捶结法 挤压法 切压法 顶压法 握压法 直取法
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家畜消化道阻塞的疗法
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作者 莫楠 《农村科技》 2004年第12期30-30,共1页
食道阻塞多因家畜偷吃饲料、粮食、蔬菜被发现后,遭追打惊吓而致,阻塞物有软有硬,阻塞部位分为上、中、下部,治疗手法也有所不同。
关键词 家畜 消化道阻塞 治疗 直取法 按摩法 推送法 黄酒法
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A Rapid Extraction Procedure for the Determination of Strontium-90 in Water Samples
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作者 Markus Zehringer Johannes Abraham +2 位作者 Franziska Kammerer Vlora Syla Michael Wagmann 《Journal of Chemistry and Chemical Engineering》 2017年第3期116-123,共8页
A beta spectrometric method for the rapid determination of strontium-90 (90Sr) in water samples was developed and tested. 90Sr is extracted directly from the water samples by means of a scintillator cocktail, contai... A beta spectrometric method for the rapid determination of strontium-90 (90Sr) in water samples was developed and tested. 90Sr is extracted directly from the water samples by means of a scintillator cocktail, containing the crown ether dicyclohexano-18-crown-6 as an extracting agent (STRONEX). Minimal clean-up is necessary when other beta nuclides are present, such as barium-140. These nuclides are eliminated by a barium chromate scavenge, prior to the extraction step. The method presented here allows for the investigation of at least 12 water samples a day and is therefore a suitable tool for emergency analysis. 展开更多
关键词 STRONTIUM-90 beta spectrometry emergency analysis STRONEX.
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Joint Non-Orthogonal Multiple Access (NOMA) & Walsh-Hadamard Transform: Enhancing the Receiver Performance 被引量:2
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作者 Muhammad Rehan Usman Arsla Khan +1 位作者 Muhammad Arslan Usman Soo young Shin 《China Communications》 SCIE CSCD 2018年第9期160-177,共18页
Non-orthogonal multiple access(NOMA) is a new access method to achieve high performance gains in terms of capacity and throughput, so it is currently under consideration as one of the candidates for fifth generation(5... Non-orthogonal multiple access(NOMA) is a new access method to achieve high performance gains in terms of capacity and throughput, so it is currently under consideration as one of the candidates for fifth generation(5 G) technologies. NOMA utilizes power domain in order to superimpose signals of multiple users in a single transmitted signal. This creates a lot of interference at the receive side. Although the use of successive interference cancellation(SIC) technique reduces the interference, but to further improve the receiver performance, in this paper, we have proposed a joint Walsh-Hadamard transform(WHT) and NOMA approach for achieving better performance gains than the conventional NOMA. WHT is a well-known code used in communication systems and is used as an orthogonal variable spreading factor(OVSF) in communication systems. Application of WHT to NOMA results in low bit error rate(BER) and high throughput performance for both low and high channel gain users. Further, it also reduces peak to average power ratio(PAPR) of the user signal. The results are discussed in terms of comparison between the conventionalNOMA and the proposed technique, which shows that it offers high performance gains in terms of low BER at different SNR levels, reduced PAPR, high user throughput performance and better spectral efficiency. 展开更多
关键词 Bit error rate (BER) non-orthogonal multiple access (NOMA) orthogonal frequency division multiplexing (OFDM) peak to average power ratio (PAPR) successive interference cancellation (SIC) throughput and Walsh-Hadamard transform (WHT).
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Objects Description and Extraction by the Use of Straight Line Segments in Digital Images
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作者 Vladimir Volkov Rudolf Germer +1 位作者 Alexandr Oneshko Denis Oralov 《Computer Technology and Application》 2011年第12期939-947,共9页
An advanced edge-based method of feature detection and extraction is developed for object description in digital images. It is useful for the comparison of different images of the same scene in aerial imagery, for des... An advanced edge-based method of feature detection and extraction is developed for object description in digital images. It is useful for the comparison of different images of the same scene in aerial imagery, for describing and recognizing categories, for automatic building extraction and for finding the mutual regions in image matching. The method includes directional filtering and searching for straight edge segments in every direction and scale, taking into account edge gradient signs. Line segments are ordered with respect to their orientation and average gradients in the region in question. These segments are used for the construction of an object descriptor. A hierarchical set of feature descriptors is developed, taking into consideration the proposed straight line segment detector. Comparative performance is evaluated on the noisy model and in real aerial and satellite imagery. 展开更多
关键词 Object recognition local descriptors affine and scale invariance edge-based feature detector feature-based imagematching building extraction.
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