大岩桐花萼切块离体培养直接再生花芽的形态观察(简报)

The morphological changes in the cultures of sepal segments in Sinningia speciosa Hiern were observed with Zeiss Stemi 2000-C Stereomicroscope from 0 to 65 days after culture in vitro. The light yellow globular protuberances were observed on the cut edge and the surface of sepal segments after culture for 24 days. Then the globular protuberances grew bigger gradually. A lot of floral buds on the surface of sepal segments formed after culture for 60 days. The morphological changes in the cultures of sepal segments were studied with light microscopy after culture for 0 to 30 days as well. The cells of tissues of sepal segments arranged regularly and no dividing cell was observed on the first day culture. There appeared some small meristematic centers of dividing cells on cut edge and the lower epidermis on the 7th day after culture. To the 20th day culture in vitro, the floral organ primordia were differentiated on the cut edge. On the 30th day culture, floral buds with petal, stamen primordia were observed. In addition, the morphological changes in the cultures of sepal segment were studied during the 14 to 30 days culture with scanning microscopy as well.

诱导风信子再生花芽不断分化花被片的研究

通过外源激素及外植体年龄的控制，诱导风信子（ Ｈｙａｃｉｎｔｈｕｓ ｏｒｉｅｎｔａｌｉｓ Ｌ．） 再生花芽不断分化花被片已经获得成功。在２５０ ｄ 的继代培养中平均每个花芽可分化７０ 多片花被片，最多的可分化１４０ 多片。对这种花芽不断分化花被片的形态发生过程以及生长发育特点的观察表明，花芽的第１ 轮器官与风信子野生型花基本相同，是花被，它由花被筒及其上部的裂片———花被片组成。第２ 轮和第３ 轮器官在野生型中应该是雄蕊和雌蕊，但再生花芽仍然分化花被片。这种花芽的生长与发育的速度在开始的几个月中较快， 以后随培养天数增加而不断减慢。继代培养１５０ ｄ 以后，花芽生长与器官分化肉眼已很难鉴别。在再生花芽的发育中除分化花被片外还在花芽内部不确定的位置上分化出一些新的小花芽，这些内部的小花芽也不断地分化花被片。组织学观察表明，再生花芽由花被外植体内侧的表皮及皮下几层中的一些细胞共同起源。内部的小花芽由刚分化的花被片基部的薄壁细胞转化为分生组织而起源。对风信子再生花芽不断分化花被片的表现型与拟南芥中ＡＧ 基因突变后ａｇａｍｏｕｓ 花的表现型的异同作了比较与讨论。

大岩桐花瓣切块离体培养高频率花芽再生(英文)

该文报道了大岩桐花瓣切块离体培养再生花现象,花瓣切块再生花有两种方式:一种是仅再生花芽(命名为BF);另一种是既再生花芽也再生营养芽(命名为BF+V)。花芽再生的能力与光照、花芽大小及培养基中赤霉素和细胞分裂素浓度紧密相关。当培养基中含有1.0 mg/L GA3时,BA的添加会显著增加总花芽(BF+BF+V)的形成率,添加0.5 mg/L BA时,总花芽形成率达100%。在暗中培养时,BF达93.4%。不同大小花芽的花瓣再生花的能力不同,7 mm直径花芽的BF最高,达86.7%。同时,对花芽再生过程中花瓣切块的组织结构形态变化也进行了观察。