低位直肠癌保肛术后复发原因与再治疗分析

目的:探讨低位直肠癌保肛术后复发的原因及再治疗的对策。方法:对在我院行低位直肠癌保肛术后复发患者32例的临床资料进行回顾性分析。结果:复发主要原因有远端切除直肠距离不足、肠外侧方淋巴结清除不彻底、肿瘤细胞的淋巴、血液和种植转移、术中操作不当或手术方式选择错误以及术后不规则放化疗。结论:低位直肠癌手术治疗后复发率高,术前应做好充分术前准备选择正确手术方式,术中应对相关淋巴结进行彻底清扫以免遗漏癌,术后仍需定期随访和规律放化疗。

AKIP1对结直肠癌细胞凋亡的影响及其分子机制研究

【目的】探究AKIP1对人结直肠癌细胞HCT-116凋亡的影响及其相关分子机制。【方法】设计AKIP1干扰片段siRNA及NC片段,转染至HCT-116细胞,Real-time PCR及Western Blot检测AKIP1 mRNA及蛋白的表达;通过MTT及流式细胞术检测细胞活力及凋亡情况;通过Real-time PCR检测细胞凋亡相关基因的表达变化,并通过Western Blot检测NF-κB p65在胞浆和胞核中的表达以及凋亡相关蛋白的表达。【结果】转染AKIP1 siRNA后,AKIP1 mRNA及蛋白的表达水平显著下调;MTT实验结果显示AKIP1 siRNA可显著抑制HCT-116细胞的活力;流式细胞术结果表明AKIP1 siRNA可显著促进HCT-116细胞凋亡;同时干扰AKIP1可上调Bax、cleaved-Caspase-3并下调Bcl-2的表达;另外,AKIP1 siRNA可下调细胞核NF-κB p65的表达,上调细胞浆中的NF-κB p65蛋白表达。【结论】AKIP1可能通过活化NF-κB p65抑制HCT-116细胞凋亡进而促进结直肠癌的疾病进展。

大肠腺瘤恶变的相关因素探讨

目的 探讨大肠腺瘤恶变的相关因素。方法 回顾性分析 14年间 2 76例大肠腺瘤的临床资料。结果 左半结肠腺瘤癌变率高于右半结肠 (P <0 .0 5 ) ;大于 2cm腺瘤癌变率高于小于 2cm者 (P <0 .0 1) ;绒毛状腺瘤癌变率高于管状和混合性腺瘤 (P <0 .0 1) ;重度不典型增生腺瘤的癌变率明显高于轻度、中度不典型增生 (P <0 .0 1) ;多发腺瘤癌变率高于单发 (P <0 .0 5 ) ;腺瘤表面炎症重、糜烂、分叶、无蒂者癌变率高于表面光滑 ,长蒂者 (P <0 .0 1)。结论 腺瘤的组织学类型、不典型增生程度以及肿瘤大小、数目、部位、年龄等诸多因素是腺瘤恶变相关的危险因素。

Association of T cell factor-4 mRNA expression with clinicopathological characteristics of colorectal cancer

Objective： To study the expression of T-cell transcription factor-4 （Tcf-4） gene in human colorectal cancer （CRC） and the relationship between Tcf-4 gene and progression of CRC. Methods： Quantitative real time RT-PCR was used to de- tect the expression of Tcf-4 mRNA in 31 CRC and para-cancerous tissues. The correlation of expression of the Tcf-4 gene with clinicopathologicel characteristics of CRC was also analyzed. Results： Compared with normal tissues, up-regulation of Tcf4 mRNAwas observed in 31 CRC tissues （t = 2.188, P = 0.037）. The expression values of Tcf-4 mRNA in Dukes A, B, C and D were 8.38 ＋ 1.92, 6.93 ＋ 1.44, 6.00 ＋ 1.26 and 3.50 ＋ 0.71 respectively. Analyzed by one-way ANOVA, there were significant differences in the expressions of Tcf-4 in different Dukes stages （F = 6.454, P = 0.002）. Moreover, the over-expression of Tcf-4 was significantly correlated with lymph node metastases. However the expression of Tcf-4 was no correlation with the age, gender of patients and cell differentiation of CRC in this study. Conclusion： The higher expression level of Tcf-4 mRNA in CRC tissues suggested that the over-expression of Tcf-4 gene may be related to the development and invasion of CRC.