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荧光法研究抗甲亢药物硫脲嘧啶与蛋白质的相互结合作用 被引量:5
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作者 谭亮 谢青季 姚守拙 《湖南师范大学自然科学学报》 EI CAS 北大核心 2003年第1期59-63,共5页
研究了硫脲嘧啶 (TU)对牛血清白蛋白 (BSA)内源荧光的猝灭效应 .在磷酸缓冲溶液 (pH7.2 ,2 0℃ )中 ,F0 F与硫脲嘧啶浓度在 4.0 0× 10 - 6 ~ 6 .0 0× 10 - 5mol L范围内存在线性关系 ,检测下限为 1.81× 10 - 7mol L ,... 研究了硫脲嘧啶 (TU)对牛血清白蛋白 (BSA)内源荧光的猝灭效应 .在磷酸缓冲溶液 (pH7.2 ,2 0℃ )中 ,F0 F与硫脲嘧啶浓度在 4.0 0× 10 - 6 ~ 6 .0 0× 10 - 5mol L范围内存在线性关系 ,检测下限为 1.81× 10 - 7mol L ,硫脲嘧啶与牛血清白蛋白分子结合比例为 1∶1,结合常数为 1.35× 10 5L mol.它们之间存在能量转移 ,求算出硫脲嘧啶与牛血清白蛋白色氨酸残基之间距离为 0 .95nm .它们之间结合反应的热力学函数分别为 :ΔrHm =5 5 .7kJ mol,ΔrSm =2 88J (mol·K) . 展开更多
关键词 荧光法 抗甲亢药物 相互结合作用 硫脲嘧啶 牛血清白蛋白 荧光猝灭 能量转移 结合比例
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RNA结合基序单链相互作用蛋白3在人乳头瘤病毒16型相关宫颈癌细胞和鳞状细胞癌组织中的表达 被引量:1
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作者 李航 崔静 +5 位作者 田明振 冯思佳 杨璐 赵二趁 原志庆 千新来 《新乡医学院学报》 CAS 2015年第8期722-724,727,共4页
目的探讨RNA结合基序单链相互作用蛋白3(RBMS3)在人乳头瘤病毒16型(HPV16)相关宫颈癌细胞和鳞状细胞癌组织中的表达。方法通过Western blotting方法检测RBMS3在HPV16 E7表达阳性和阴性宫颈癌细胞中的表达,通过免疫组织化学(IHC)染色方... 目的探讨RNA结合基序单链相互作用蛋白3(RBMS3)在人乳头瘤病毒16型(HPV16)相关宫颈癌细胞和鳞状细胞癌组织中的表达。方法通过Western blotting方法检测RBMS3在HPV16 E7表达阳性和阴性宫颈癌细胞中的表达,通过免疫组织化学(IHC)染色方法检测RBMS3在HPV16 E7表达阳性和阴性宫颈鳞状细胞癌组织中的表达,并分析RBMS3和HPV16 E7表达的关系。结果 Western blotting结果显示,HPV16 E7在宫颈癌C33-A细胞中不表达,而HPV16 E7在宫颈癌Si Ha细胞中表达;C33-A细胞中RBMS3的表达显著高于Si Ha细胞(P<0.01)。IHC结果显示,RBMS3在HPV16 E7表达阳性宫颈鳞状细胞癌组织中的表达显著低于HPV16 E7表达阴性宫颈鳞状细胞癌组织(P<0.01),RBMS3和HPV16 E7表达呈负相关(P<0.01)。结论 HPV16相关宫颈鳞状细胞癌组织中RBMS3的表达与HPV16 E7的表达呈负相关,RBMS3可能与HPV16相关宫颈癌的发生有关。 展开更多
关键词 宫颈鳞状细胞癌 人乳头瘤病毒 RNA结合基序单链相互作用蛋白3
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富氢水对糖尿病视网膜病变大鼠硫氧还蛋白相互作用蛋白/核苷酸结合寡聚化结构域样受体蛋白3通路及视网膜血管通透性的影响 被引量:3
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作者 冯梅 江霞 +1 位作者 王艳丽 王奇峰 《安徽医药》 CAS 2022年第7期1367-1373,I0003,共8页
目的 探究富氢水对糖尿病视网膜病变大鼠视网膜血管通透性及硫氧还蛋白相互作用蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)通路的影响。方法 2020年6—9月,SD大鼠采用随机数字表法选取15只为空白组,其余大鼠尾静脉注射链脲... 目的 探究富氢水对糖尿病视网膜病变大鼠视网膜血管通透性及硫氧还蛋白相互作用蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)通路的影响。方法 2020年6—9月,SD大鼠采用随机数字表法选取15只为空白组,其余大鼠尾静脉注射链脲佐菌素(STZ,60 mg/kg)和高脂饲料喂养构建DR模型,造模成功的大鼠采用随机数字表法分为模型组、富氢水低、高剂量组(5、10 mL/kg),每组15只。连续给药28 d后,记录大鼠体质量,检测空腹血糖(FBG)水平;眼底照相和荧光素血管造影(FFA)观察大鼠右眼新生血管和荧光素渗漏现象;光学相干断层扫描(OCT)检测视网膜厚度;伊文思蓝-白蛋白复合物渗漏分析视网膜血管通透性;HE染色观察视网膜病理变化;免疫荧光染色观察新生血管形成情况;TUNEL染色检测视网膜细胞凋亡;酶联免疫吸附(ELISA)试剂盒测量视网膜匀浆中血管生成素-1(Ang-1)、血管内皮生长因子(VEGF)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)水平;WB法检测视网膜TXNIP/NLRP3通路和凋亡相关蛋白的表达。结果 与空白组相比,模型组大鼠FBG[(26.49±2.18)mmol/L比(5.76±1.09)mmol/L]、血-视网膜屏障(BRB)通透性[(32.51±2.05)μg/g比(11.24±1.76)μg/g]、视网膜细胞凋亡[(23.31±2.14)%比(0.07±0.01)%]、Bcl-2相关X蛋白(Bax)、B淋巴细胞瘤-2(Bcl-2)、TXNIP[(0.85±0.09)比(0.40±0.05)]、NLRP3[(0.93±0.10)比(0.48±0.07)]、IL-1β[(0.74±0.05)比(0.41±0.04)]、胱天蛋白酶-1(caspase-1)[(0.78±0.07)比(0.24±0.04)]表达、Bax/Bcl-2比值、视网膜匀浆中Ang-1、VEGF、IL-1β、IL-6水平显著增加(P<0.05),体质量、视网膜厚度[(148.31±12.76)μm比(223.57±17.23)μm]显著降低(P<0.05),视网膜有较多新生血管和血管曲折,血管渗漏严重,视网膜细胞明显水肿,有大量炎性渗出,神经节细胞层(GCL)细胞数量减少;与模型组相比,富氢水低、高剂量组大鼠FBG[(20.85±3.45)mmol/L、(14.27±2.42)mmol/L比(26.49±2.18)mmol/L]、BRB通透性[(24.67±1.85)μg/g、(17.48±1.63)μg/g比(32.51±2.05)μg/g]、视网膜细胞凋亡[(14.65±1.36)%、(9.85±1.22)%比(23.31±2.14)%]、Bax、Bcl-2、TXNIP[(0.64±0.09)、(0.52±0.08)比(0.85±0.09)]、NLRP3[(0.72±0.09)、(0.61±0.08)比(0.93±0.10)]、IL-1β[(0.62±0.06)、(0.53±0.05)比(0.74±0.05)]、caspase-1[(0.57±0.06)、(0.41±0.05)比(0.78±0.07)]表达、Bax/Bcl-2比值、视网膜匀浆中Ang-1、VEGF、IL-1β、IL-6水平明显降低(P<0.05),体质量、视网膜厚度[(173.62±14.46)μm、(196.54±15.75)μm比(148.31±12.76)μm]明显增加(P<0.05),视网膜水肿情况减轻,GCL细胞数量明显增加,损伤得到改善。结论 富氢水可抑制新生血管形成,降低DR大鼠视网膜血管通透性,减轻视网膜神经元损伤;其作用机制可能与抑制TXNIP/NLRP3通路的激活有关。 展开更多
关键词 糖尿病视网膜病变 富氢水 血-视网膜屏障 硫氧还蛋白相互作用蛋白/核苷酸结合寡聚化结构域样受体蛋白3 大鼠 Sprague-Dawley
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多聚腺苷酸结合蛋白相互作用蛋白1与肿瘤关系的研究进展
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作者 罗胜茂 易世江 凌月福 《现代肿瘤医学》 CAS 北大核心 2021年第16期2918-2922,共5页
多聚腺苷酸结合蛋白相互作用蛋白1[Poly(A)-binding protein-interacting protein 1,Paip1]是哺乳动物特有的蛋白质,可有效参与真核生物基因表达及细胞周期调控,并与肿瘤演进及预后密切相关。近年来Paip1成为肿瘤研究热点,Paip1与肿瘤... 多聚腺苷酸结合蛋白相互作用蛋白1[Poly(A)-binding protein-interacting protein 1,Paip1]是哺乳动物特有的蛋白质,可有效参与真核生物基因表达及细胞周期调控,并与肿瘤演进及预后密切相关。近年来Paip1成为肿瘤研究热点,Paip1与肿瘤细胞增殖、迁移、侵袭和上皮间质转化进程密切相关,可能是肿瘤进展及预后不良的潜在生物标志和新的治疗靶点。但其具体作用机制尚未阐明。本文就Paip1的结构、功能、泛素化和降解及其与肿瘤的关系等方面作一综述,为其在肿瘤中的研究提供新思路。 展开更多
关键词 多聚腺苷酸结合蛋白相互作用蛋白1 信号通路 基因表达调控 细胞周期 肿瘤
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Paip1在翻译过程中的作用及机制研究进展 被引量:2
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作者 吴水梅 刘茂生 +3 位作者 郑燕 李琳琳 李腾政 张吉翔 《中国老年学杂志》 CAS CSCD 北大核心 2016年第8期2009-2011,共3页
多聚腺苷酸结合相互作用蛋白1(Paip1)是哺乳动物特有的蛋白质,与多聚腺苷酸结合蛋白(PABP)及真核生物翻译起始因子4A(e IF4A)相互作用,参与了翻译起始及蛋白质的生物合成,然而Paip1调节翻译的具体机制还不是很清楚,我们将目前PAi... 多聚腺苷酸结合相互作用蛋白1(Paip1)是哺乳动物特有的蛋白质,与多聚腺苷酸结合蛋白(PABP)及真核生物翻译起始因子4A(e IF4A)相互作用,参与了翻译起始及蛋白质的生物合成,然而Paip1调节翻译的具体机制还不是很清楚,我们将目前PAi P1相关的研究进展做如下归纳。 展开更多
关键词 多聚腺苷酸结合相互作用蛋白1 多聚腺苷酸结合蛋白 真核生物翻译起始因子
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RBMS3-AS3对宫颈癌细胞增殖、凋亡和侵袭的影响及作用机制 被引量:1
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作者 兰莉 陈海丽 徐蕾 《癌变.畸变.突变》 CAS 2020年第6期438-443,共6页
目的:探讨RNA结合基序单链相互作用蛋白3反义链3(RBMS3-AS3)对宫颈癌细胞增殖、凋亡和侵袭的影响及作用机制。方法:培养人宫颈永生化细胞Ect1/E6E7和宫颈癌HeLa、Caski和SiHa细胞,实时荧光定量PCR检测细胞中RBMS3-AS3表达水平,Western b... 目的:探讨RNA结合基序单链相互作用蛋白3反义链3(RBMS3-AS3)对宫颈癌细胞增殖、凋亡和侵袭的影响及作用机制。方法:培养人宫颈永生化细胞Ect1/E6E7和宫颈癌HeLa、Caski和SiHa细胞,实时荧光定量PCR检测细胞中RBMS3-AS3表达水平,Western blot法检测RNA结合基序单链相互作用蛋白3(RBMS3)蛋白水平。将HeLa细胞分为对照组(细胞正常培养)、si-RBMS3-AS3组(转染RBMS3-AS3小干扰RNA)、阴性序列组(转染乱序无意义阴性序列)、si-RBMS3-AS3+si-RBMS3组(共转染RBMS3-AS3和RBMS3的小干扰RNA)和si-RBMS3-AS3+阴性序列组(共转染RBMS3-AS3与乱序无意义阴性序列),四甲基噻唑蓝染色法(MTT)检测细胞增殖率,流式细胞术检测细胞凋亡率,Transwell检测细胞侵袭能力,Western blot检测各组HeLa细胞中细胞周期蛋白D1(Cyclin D1)、B淋巴细胞瘤-2(Bcl-2)、B淋巴细胞瘤-2相关蛋白(Bax)和基质金属蛋白酶2(MMP-2)蛋白表达水平。结果:宫颈癌细胞系HeLa、Caski和SiHa中RBMS3-AS3表达水平均高于Ect1/E6E7细胞(P<0.05),而RBMS3蛋白表达低于Ect1/E6E7细胞(P<0.05)。与对照组或阴性序列组相比,si-RBMS3-AS3组HeLa细胞的活性、侵袭细胞数、Cyclin D1、Bcl-2和MMP-2蛋白表达水平降低(P<0.05),细胞凋亡率及RBMS3和Bax蛋白表达水平升高(P<0.05)。与si-RBMS3-AS3+阴性序列组比较,si-RBMS3-AS3+si-RBMS3组HeLa细胞活性、侵袭细胞数及Cyclin D1、Bcl-2和MMP-2蛋白表达水平升高(P<0.05),细胞凋亡率和Bax蛋白表达水平降低(P<0.05)。结论:抑制RBMS3-AS3表达可抑制宫颈癌细胞增殖和侵袭,并促进细胞凋亡,这可能与上调RBMS3表达有关。 展开更多
关键词 RNA结合基序单链相互作用蛋白3 RNA结合基序单链相互作用蛋白3反义链3 宫颈癌 细胞增殖 凋亡 侵袭
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基于TXNIP/NLRP3炎性小体通路研究清热凉血方对溃疡性结肠炎大鼠肠黏膜屏障的保护作用 被引量:3
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作者 范咸玉 邹建文 +1 位作者 陈旭 吴刚 《中国老年学杂志》 CAS 北大核心 2023年第18期4563-4567,共5页
目的基于硫氧还蛋白相互作用蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体蛋白(NLRP)3炎性小体通路探讨清热凉血方(QRLX)对溃疡性结肠炎(UC)大鼠肠黏膜屏障的保护作用及潜在机制。方法78只大鼠随机选取12只为对照(Control)组,其余大鼠采... 目的基于硫氧还蛋白相互作用蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体蛋白(NLRP)3炎性小体通路探讨清热凉血方(QRLX)对溃疡性结肠炎(UC)大鼠肠黏膜屏障的保护作用及潜在机制。方法78只大鼠随机选取12只为对照(Control)组,其余大鼠采用葡聚糖硫酸钠(DSS)构建UC大鼠模型,将造模成功大鼠随机分为UC组、柳氮磺吡啶组(SASP,270 mg/kg)、清热凉血方低(QRLX-L,14.8 g/kg)、中(QRLX-M,29.6 g/kg)、高剂量(QRLX-H,59.2 g/kg)组,每组12只。各给药组给予相应剂量药物灌胃,连续2 w。异硫氰酸荧光素-葡聚糖(FITC-dextran)灌胃检测肠道渗透性;酶联免疫吸附试验(ELISA)检测血浆中二胺氧化酶(DAO)活性、D乳酸(LA)、内毒素(ET)和结肠组织白细胞介素(IL)-1β、IL-18水平;苏木素-伊红(HE)染色观察结肠组织病理学变化;免疫组化法检测肠屏障功能相关紧密连接蛋白(ZO)-1和occludin表达;Western印迹检测结肠组织TXNIP/NLRP3通路相关蛋白表达。结果与Control组相比,UC组血清FITC-dextran浓度、血浆DAO活性、D-LA、ET、结肠匀浆中IL-1β、IL-18水平、TXNIP、NLRP3、切割型半胱氨酸天冬氨酸蛋白水解酶(cl-caspase)-1 p10、cl-caspase-1 p20蛋白水平明显升高,结肠组织occludin、ZO-1阳性表达明显减少(均P<0.05);与UC组相比,SASP组和QRLX-H、QRLX-M组FITC-dextran浓度、血浆DAO活性、D-LA、ET、结肠匀浆中IL-1β、IL-18水平、TXNIP、NLRP3、cl-caspase-1 p10、cl-caspase-1 p20蛋白水平明显降低,结肠组织occludin-1、ZO-1阳性表达明显增加(均P<0.05)。结论QRLX可增强UC大鼠的肠黏膜屏障功能,改善UC;其作用机制可能与抑制TXNIP/NLRP3炎性小体通路的激活有关。 展开更多
关键词 清热凉血方 溃疡性结肠炎 肠黏膜屏障功能 硫氧还蛋白相互作用蛋白/核苷酸结合寡聚化结构域样受体蛋白3
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Computational Simulation of Aptamer-target Binding Mechanisms
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作者 YANG Yuan-Yuan XU Fei WU Xiu-Xiu 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2024年第11期1550-1562,共13页
Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as bioch... Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as biochemical sensors for medicine,food safety and environmental monitoring.However,there is little research on aptamer-target binding mechanisms,which limits their application and development.Computational simulation has gained much attention for revealing aptamer-target binding mechanisms at the atomic level.This work summarizes the main simulation methods used in the mechanistic analysis of aptamer-target complexes,the characteristics of binding between aptamers and different targets(metal ions,small organic molecules,biomacromolecules,cells,bacteria and viruses),the types of aptamer-target interactions and the factors influencing their strength.It provides a reference for further use of simulations in understanding aptamer-target binding mechanisms. 展开更多
关键词 computational simulation APTAMER TARGET binding mechanism intermolecular forces
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Zonal Coupling Analysis Method of Seismic Response of Offshore Monopile Wind Turbine
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作者 XU Xiaofeng CHEN Shaolin SUN Jie 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2024年第S01期103-110,共8页
The seismic safety of offshore wind turbines is an important issue that needs to be solved urgently.Based on a unified computing framework,this paper develops a set of seawater-seabed-wind turbine zoning coupling anal... The seismic safety of offshore wind turbines is an important issue that needs to be solved urgently.Based on a unified computing framework,this paper develops a set of seawater-seabed-wind turbine zoning coupling analysis methods.A 5 MW wind turbine and a site analysis model are established,and a seismic wave is selected to analyze the changes in the seismic response of offshore monopile wind turbines under the change of seawater depth,seabed wave velocity and seismic wave incidence angle.The analysis results show that when the seawater increases to a certain depth,the seismic response of the wind turbine increases.The shear wave velocity of the seabed affects the bending moment and displacement at the bottom of the tower.When the angle of incidence increases,the vertical displacement and the acceleration of the top of the tower increase in varying degrees. 展开更多
关键词 offshore monopile wind turbine seismic response analysis soil-junction interactions fluid-structure inter-action
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多聚腺苷酸结合蛋白相互作用蛋白1在胶质瘤的预后价值及其对免疫微环境的影响
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作者 孙晨 张晓倩 丁雪鹰 《中华实验外科杂志》 CAS 北大核心 2023年第9期1707-1710,共4页
目的探讨多聚腺苷酸结合蛋白相互作用蛋白1(PAIP1)在胶质瘤的预后价值及其对肿瘤免疫的影响。方法在癌症基因组图谱(TCGA)数据库下载得到泛癌转录组数据及患者临床数据,比较PAIP1在泛癌中的表达差异性。通过免疫组织化学方法检测胶质瘤P... 目的探讨多聚腺苷酸结合蛋白相互作用蛋白1(PAIP1)在胶质瘤的预后价值及其对肿瘤免疫的影响。方法在癌症基因组图谱(TCGA)数据库下载得到泛癌转录组数据及患者临床数据,比较PAIP1在泛癌中的表达差异性。通过免疫组织化学方法检测胶质瘤PAIP1表达情况。通过Kaplan-Meier分析PAIP1表达对胶质瘤患者预后的影响及其与胶质瘤患者临床病理特征的关系。利用GO功能与KEGG通路富集分析PAIP1在胶质瘤的潜在作用。单样本GSEA(ssGSEA)免疫浸润算法分析胶质瘤微环境免疫细胞浸润网络。利用Wilcoxon检验或T检验进行组间差异分析,Spearman检验进行相关性分析。结果胶质瘤组织中PAIP1表达量高于癌旁组织(5.386±0.277比5.858±0.455,P<0.05),高表达PAIP1的胶质瘤患者肿瘤恶性程度较高,总体生存期[风险比(HR)=2.95,P<0.01]和疾病特异生存期(HR=2.91,P<0.01)均显著缩短。免疫组织化学结果显示,胶质瘤组PAIP1表达量高于阴染组(11.460%比7.650%,t=4.917,P<0.001)。功能分析结果提示,PAIP1可能参与肿瘤细胞周期及肿瘤免疫的调控。高表达PAIP1的胶质瘤患者辅助型T细胞2浸润程度(0.337±0.033比0.383±0.037)和白细胞介素(IL)-4的表达量高于低表达PAIP1的胶质瘤患者(R=0.194,P<0.01)。结论PAIP1高表达与胶质瘤预后不良相关,可能通过促进Th2细胞浸润及IL-4分泌参与肿瘤免疫调控。 展开更多
关键词 多聚腺苷酸结合蛋白相互作用蛋白1 胶质瘤 预后 免疫微环境 辅助型T细胞2
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XPS AND UPS STUDIES OF THE INTERFACIAL INTERACTION IN Ni ZrO 2 COMPOSITE PLATING
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作者 王为 项民 +2 位作者 郭鹤桐 覃奇贤 任殿胜 《Transactions of Tianjin University》 EI CAS 1998年第1期98-101,共4页
The interfacial interaction existing in the Ni ZrO 2 composite plating has been investigated. The experimental results show that no new phases were formed in the interfacial regions between matrix Ni and ZrO 2 part... The interfacial interaction existing in the Ni ZrO 2 composite plating has been investigated. The experimental results show that no new phases were formed in the interfacial regions between matrix Ni and ZrO 2 particles, but an orbital interaction through the mutual overlap of the d orbits does exist in the interfacial regions between Ni atoms and Zr 3+ ions. 展开更多
关键词 Ni ZrO 2 composite plating interfacial structure INTERACTION
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胃腺癌组织RBMS1、AKAP12表达变化及其临床意义
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作者 房志学 叶凡 +2 位作者 周宇帆 周轲 李琢 《山东医药》 CAS 2023年第13期20-23,39,共5页
目的探讨胃腺癌组织RNA结合基序单链相互作用蛋白1(RBMS1)、A激酶锚定蛋白12(AKAP12)表达变化及其临床意义。方法选择胃腺癌患者102例,取手术切除的胃癌组织及其癌旁组织(距肿瘤组织边缘5 cm以上,经病理检查明确为正常胃组织),采用免疫... 目的探讨胃腺癌组织RNA结合基序单链相互作用蛋白1(RBMS1)、A激酶锚定蛋白12(AKAP12)表达变化及其临床意义。方法选择胃腺癌患者102例,取手术切除的胃癌组织及其癌旁组织(距肿瘤组织边缘5 cm以上,经病理检查明确为正常胃组织),采用免疫组化法检测RBMS1、AKAP12表达。比较胃癌组织与癌旁正常组织RBMS1、AKAP12阳性表达率,采用Pearson线性相关分析法分析胃癌组织RBMS1阳性表达与AKAP12阳性表达的关系。分析胃癌组织RBMS1、AKAP12阳性表达与临床病理特征的关系以及二者表达与预后的关系。结果胃癌组织RBMS1阳性表达率显著高于癌旁正常组织,AKAP12阳性表达率显著低于癌旁正常组织(χ^(2)分别为75.583、53.204,P均<0.05)。胃癌组织RBMS1阳性表达与AKAP12阳性表达呈负相关关系(r=-0.625,P<0.05)。胃癌组织RBMS1、AKAP12阳性表达与TNM分期、淋巴结转移有关(P均<0.05),与性别、年龄、肿瘤最大径、肿瘤部位、组织分化程度无关(P均>0.05)。所有患者术后随访2~36个月,中位随访时间25.30个月。随访期间失访1例,死亡30例,3年总体生存率为70.30%(71/101)。胃癌组织RBMS1阳性表达者与阴性表达者3年总体生存率分别为66.20%(47/71)、80.00%(24/30),胃癌组织AKAP12阳性表达者与阴性表达者3年总体生存率分别为82.14%(23/28)、65.75%(48/73)。胃癌组织RBMS1阳性表达者3年总体生存率显著低于其阴性表达者(χ^(2)=4.310,P<0.05),胃癌组织AKAP12阳性表达者3年总体生存率显著高于其阴性表达者(χ^(2)=4.569,P<0.05)。结论胃腺癌组织RBMS1高表达、AKAP12低表达,二者表达变化与肿瘤TNM分期、淋巴结转移和患者预后密切相关。 展开更多
关键词 胃腺癌 RNA结合基序单链相互作用蛋白1 A激酶锚定蛋白12 临床病理特征 预后
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Theoretical Investigating Mechanisms of Drug-Resistance Generated by Mutation-Induced Changes in Influenza Viruses 被引量:1
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作者 Song Luo Xiaoyu Zhao +1 位作者 Yihui Wang Lili Duan 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2021年第6期785-796,I0003,I0061-I0067,共20页
Influenza A(A/H_(x)N_(y))is a significant public health concern due to its high infectiousness and mortality.Neuraminidase,which interacts with sialic acid(SIA)in host cells,has become an essential target since its hi... Influenza A(A/H_(x)N_(y))is a significant public health concern due to its high infectiousness and mortality.Neuraminidase,which interacts with sialic acid(SIA)in host cells,has become an essential target since its highly conserved catalytic center structure,while resistance mutations have already generated.Here,a detailed investigation of the drug resistance mechanism caused by mutations was performed for subtype N9(A/H7N9).Molecular dynamics simulation and alanine-scanning-interaction-entropy method(ASIE)were used to explore the critical differences between N9 and Zanamivir(ZMR)before and after R294K mutation.The results showed that the mutation caused the hydrogen bond between Arg294 and ZMR to break,then the hydrogen bonding network was disrupted,leading to weakened binding ability and resistance.While in wild type(A/H7N9^(WT)),this hydrogen bond was initially stable.Meanwhile,N9 derived from A/H11N9 was obtained as an R292K mutation.Then the relative binding free energy of N9 with five inhibitors(SIA,DAN,ZMR,G28,and G39)was predicted,basically consistent with experimental values,indicating that the calculated results were reliable by ASIE.In addition,Arg292 and Tyr406 were hot spots in the A/H11N9^(WT)-drugs.However,Lys292 was not observed as a favorable contributing residue in A/H11N9^(R292K),which may promote resistance.In comparison,Tyr406 remained the hotspot feature when SIA,ZMR,and G28 binding to A/H11N9^(R292K).Combining the two groups,we speculate that the resistance was mainly caused by the disruption of the hydrogen bonding network and the transformation of hotspots.This study could guide novel drug delivery of drug-resistant mutations in the treatment of A/H_(x)N9. 展开更多
关键词 N9 Drug resistance Alanine-scanning-interaction-entropy method Binding free energy
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Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation 被引量:1
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作者 Wei Qian Jianyong Wang +4 位作者 Na-Na Jin Xiao-Hong Fu Yi-Chien Lin Jing-Jer Lin Jin-Qiu Zhou 《Cell Research》 SCIE CAS CSCD 2009年第7期849-863,共15页
In Saccharomyces cerevisiae, the essential gene CDC13 encodes a telomeric single-stranded DNA-binding protein that interacts with Stnlp and Tenlp genetically and physically, and is required for telomere end protection... In Saccharomyces cerevisiae, the essential gene CDC13 encodes a telomeric single-stranded DNA-binding protein that interacts with Stnlp and Tenlp genetically and physically, and is required for telomere end protection and telomere length control. The molecular mechanism by which Tenl participates in telomere length regulation and chromosome end protection remains elusive. In this work, we observed a weak interaction of Cdc13p and Tenlp in a gelfiltration analysis using purified recombinant Cdc13p and Tenlp. Tenlp itself exhibits a weak DNA-binding activity, but enhances the telomeric TG1-3 DNA-binding ability of Cdc13p. Cdc13p is communoprecipitated with Tenlp. In the mutant ten1-55 or ten1-66 cells, the impaired interaction between Tenlp and Cdc13p results in much longer telomeres, as well as a decreased association of Cdc13p with telomeric DNA. Consistently, the Ten1-55 and Ten1-66 mutant proteins fail to stimulate the telomeric DNA-binding activity of Cdc13p in vitro. These results suggest that Tenlp enhances the telomeric DNA-binding activity of Cdc13p to negatively regulate telomere length. 展开更多
关键词 TELOMERE Cdc 13 Ten 1 Stn 1
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An Approximated Voxel Approach for the Identification and Modelling of Ligand-Binding Sites
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作者 Ling Wei Lee Andrzej Bargiela 《Journal of Physical Science and Application》 2012年第10期399-408,共10页
Most protein-ligand interactions take place on surfaces and include but not limited to factors such as chemical composition, hydrophobicity, electronegavitiy and shape complementarity. Past studies showed that protein... Most protein-ligand interactions take place on surfaces and include but not limited to factors such as chemical composition, hydrophobicity, electronegavitiy and shape complementarity. Past studies showed that protein-protein interactions occur on comparatively fiat regions whereas protein-ligand bindings involve crevices. In the search for such sites various approaches have been designed and developed each of which is algorithmically unique. The use of grid units or voxels has been demonstrated in early studies with relatively good results obtained. We present here an approximated approach comprising of the use of voxels and computer vision methods in the search for ligand-binding areas. Each test protein is modelled and analysed in 2D with all corresponding residues graphically presented for successfully identified sites. The study was carried out on 2 sets of proteins: FK506-bound proteins and heme-bound proteins with promising results obtained for all test cases. 展开更多
关键词 Binding sites identification LIGAND-BINDING voxel space voxelisation grid units protein surface atoms.
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Quantum Anti-Zeno Effect in Artificial Quantum Systems 被引量:1
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作者 艾清 廖洁桥 《Communications in Theoretical Physics》 SCIE CAS CSCD 2010年第12期985-996,共12页
In this paper, we study a quantum anti-Zeno effect (QAZE) purely induced by repetitive measurements for an artificial atom interacting with a structured bath. This bath can be artificially realized with coupled reso... In this paper, we study a quantum anti-Zeno effect (QAZE) purely induced by repetitive measurements for an artificial atom interacting with a structured bath. This bath can be artificially realized with coupled resonators in one dimension and possesses photonic band structure like Bloeh electron in a periodic potential. In the presence of repetitive measurements, the pure QAZE is discovered as the observable decay is not negligible even for the atomic energy level spacing outside of the energy band of the artificial bath. If there were no measurements, the decay would not happen outside of the band. In this sense, the enhanced decay is completely induced by measurements through the relaxation channels provided by the bath. Besides, we also discuss the controversial golden rule decay rates originated from the van Hove's singularities and the effects of the counter-rotating terms. 展开更多
关键词 quantum Zeno effect quantum anti-Zeno effect artificial quantum system
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Interaction mechanism between carboxylmethyl cellulose and iron ore concentrates in iron ore agglomeration 被引量:1
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作者 杨桂明 范晓慧 +3 位作者 陈许玲 袁礼顺 黄晓贤 李曦 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第4期1241-1246,共6页
Carboxylmethyl cellulose(CMC) has become a commercial organic binder in agglomeration of iron ore concentrates. The relative molecular mass and degree of substitution(DS) of CMC have a large impact on its binding perf... Carboxylmethyl cellulose(CMC) has become a commercial organic binder in agglomeration of iron ore concentrates. The relative molecular mass and degree of substitution(DS) of CMC have a large impact on its binding performance. The interaction mechanism between CMC and iron ore particles was analyzed through Zeta potential measurements, adsorption measurements and infrared spectra. The results show that the interaction is chemical adsorption-oriented and the CMC's adsorption performance is related to the properties of CMC as well as the type of iron oxides. CMC has a greater affinity to Fe2O3 than Fe3O4, and CMC with higher relative molecular mass shows a higher adsorption isotherm. Pelletization of practical iron ore concentrates added with CMC further illustrates that CMC with higher relative molecular mass or DS exhibits a better binding performance, which is consistent with the results of adsorption tests. 展开更多
关键词 carboxylmethyl cellulose iron ore concentrate interaction mechanism adsorption agglomeration
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Synthesis, Crystal Structure and Interactions with Different G-quadruplex DNA and ctDNA of Zn-CAP
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作者 Caifeng Liu Yunfeng An Yan Peng Qiping Qin Hui Zhong Chao Lu Guohai Zhang 《Journal of Chemistry and Chemical Engineering》 2014年第5期444-452,共9页
In this study, one mononuclear zinc(II) complex with 1,2-bis CAP ((5-chlorosalicylidene amino)-phenylene): C22C13N2035Znl5 H0125 (Zn-CAP) was synthesized. The binding properties of Zn-CAP with G-quadruplex DN... In this study, one mononuclear zinc(II) complex with 1,2-bis CAP ((5-chlorosalicylidene amino)-phenylene): C22C13N2035Znl5 H0125 (Zn-CAP) was synthesized. The binding properties of Zn-CAP with G-quadruplex DNA and ctDNA (calf thymus DNA) were examined by fluorescence, CD (circular dichroism) spectroscopic and FRET (fluorescence resonance energy transfer) assay. In the fluorescence emission spectral analysis, the addition of three series of G-quadruplex DNA (G4-HTG21, G4-Pu27 and G4-c-kit-l) into the Zn-CAP solution induced moderate or add hypochromicity with total quenching ratios of 10.73%, 15.07% and 8.59% in the presence of K+ were achieved, respectively. While the addition of ctDNA under same condition only caused 7.08% quenching on the fluorescence emission of Zn-CAP. In the CD spectral analysis, the interaction with Zn-CAP could induce significant spectral changes on the CD absorption of G4-HTG21, G4-Pu27 and G4-c-kit-1, with 106.00%, 93.06%, 113.47% increment at 232 nm absorption, along with a 81.11%, 92.80%, 83.72% decrement at 295 nm or 270 nm absorption, which demonstrated that the antiparallel structure of G-quadruplex DNA is more stable in the presence of Zn-CAP. Comparatively, the addition of Zn-CAP could induce significant spectral changes on the CD absorption of double helix ctDNA, with 64.17% decrement on the positive peak absorption, along with a 90.91% increment on the negative peak absorption. On the other hand, in the FRET-melting assay analysis, it was clear that Zn-CAP at 0.5 equivalences could raise the melting temperature of G-quadruplex (F2 IT or FPul 8T) by 3.45℃ and 15.85℃, indicating an obvious stabilization effect of Zn-CAP on G-quadruplex in Pu27. All the results indicated that Zn-CAP exhibited higher binding affinity and binding intensity to G-quadruplex DNA than ctDNA, especially G-quadruplex Pu27. 展开更多
关键词 Zn-CAP G-quadruplex DNA CTDNA spectroscopy FRET assay.
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丙泊酚对缺氧复氧的肠上皮细胞ROS/TXNIP/NLRP3通路及凋亡的影响 被引量:1
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作者 吴双 吴师 曾继逾 《中国药师》 CAS 2021年第9期1630-1635,共6页
目的:探讨丙泊酚处理对缺氧复氧的肠上皮细胞活性氧(ROS)/硫氧还蛋白相互作用蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体家族吡啉结构域蛋白3(NLRP3)通路及凋亡的影响。方法:对人肠上皮HIEC细胞进行培养,并进行缺氧复氧处理;通过不同浓... 目的:探讨丙泊酚处理对缺氧复氧的肠上皮细胞活性氧(ROS)/硫氧还蛋白相互作用蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体家族吡啉结构域蛋白3(NLRP3)通路及凋亡的影响。方法:对人肠上皮HIEC细胞进行培养,并进行缺氧复氧处理;通过不同浓度(25,50,100μmol·L^(-1))丙泊酚对缺氧复氧HIEC细胞进行处理,细胞计数盒8(CCK-8)法检测细胞活力;流式细胞术检测HIEC细胞凋亡;2’,7’-二氯二氢荧光素二乙酸酯(DCFH-DA)法检测HIEC细胞ROS水平;蛋白免疫印迹(WB)法检测凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶3(Caspase-3)、B细胞淋巴瘤-2(Bcl2)、Bcl-2相关基因(Bax)、及TXNIP、NLRP3、白介素-1β(IL-1β)表达情况。结果:与对照组比较,缺氧复氧组HIEC细胞活力、蛋白Bcl2表达水平显著降低,HIEC细胞凋亡率、细胞内ROS含量、蛋白Bax、Caspase-3、TXNIP、NLRP3、IL-1β表达水平显著升高(P<0.05);与缺氧复氧组比较,不同浓度丙泊酚处理组HIEC细胞活力、蛋白Bcl2表达水平显著升高,细胞凋亡率、细胞内ROS含量、蛋白Bax、Caspase-3、TXNIP、NLRP3、IL-1β表达水平显著降低(P<0.05)。结论:丙泊酚处理能够抑制缺氧复氧HIEC细胞ROS/TXNIP/NLRP3通路及细胞的凋亡,可能对肠上皮细胞缺血再灌注损伤(I/R)有一定的保护作用。 展开更多
关键词 丙泊酚 缺氧复氧 肠上皮细胞 活性氧/硫氧还蛋白相互作用蛋白/核苷酸结合寡聚化结构域样受体家族吡啉结构域蛋白3通路 凋亡
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Numerical Simulation of Geotechnical Problems by Coupled Finite and Infinite Elements
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作者 Kemal Edip Mihail Garevski +3 位作者 Christoph Butenweg Vlatko Sesov Julijana Cvetanovska Igor Gjorgiev 《Journal of Civil Engineering and Architecture》 2013年第1期68-77,共10页
in geotechnical engineering, numerical simulation of problems is of great importance. This work proposes a new formulation of coupled finite-infinite elements which can be used in numerical simulation ofgeotechnical p... in geotechnical engineering, numerical simulation of problems is of great importance. This work proposes a new formulation of coupled finite-infinite elements which can be used in numerical simulation ofgeotechnical problems in both static and dynamic conditions. Formulation and various implementation aspects of the proposed coupled finite-infinite elements are carefully discussed. To the authors' knowledge, this approach that considers coupled finite-infinite elements is more efficient in the sense that appropriate and accurate results are obtained by using less elements. The accuracy and efficiency of the proposed approach is considered by comparing the obtained results with analytical and numerical results. In a static case, the problem of circular domain ol infinite length is considered. In a dynamic case, one dimensional wave propagation problems arising from the Heaviside step fimction and impulse functions are considered. In order to get a more complete picture, two dimensional wave propagation in a circular qtmrter space is considered and the results are presented. Finally, a soil-structure interaction system subjected to seismic excitation is analyzed. In the analysis of soil-structure interaction phenomenon, frames with different number of storeys and soil media with various stiffness characteristics have been taken into consideration. In the analysis, the finite element software ANSYS has been used. For the newly developed infinite element, the programming has been done by the help of the User Programmable Features of the ANSYS software, which enable creating new elements in the ANSYS software. 展开更多
关键词 Numerical methods infinite elements soil-structure interaction wave propagation.
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