OBJECTIVE： To investigate the anticancer, antiin flammatory, and antiobesity activity of methanol extracts of eight distinct species： Artemisia Stolon ifera （AST）, Artemisia Selengensis （ASE）, Artemisia .la poni...OBJECTIVE： To investigate the anticancer, antiin flammatory, and antiobesity activity of methanol extracts of eight distinct species： Artemisia Stolon ifera （AST）, Artemisia Selengensis （ASE）, Artemisia .la ponica, Artemisia Montana, Artemisia Capillaris （ACA）, Artemisia Sylvatica （ASY）, Artemisio Keiskeana （AKE）, and Artemisia Scoparia （ASC） in vitro. METHODS： Antiproliferative activity was investigat ed in human breast cancer estrogen receptora pos itive T47D and negative HS578T cell lines exposed to the extracts at various concentrations （5200 mgl mL）for24, 48, and 72 h. For evaluating the antiin flammatory activity of the extracts, inhibition of ni trite synthesis was investigated in lipopolysaccha ride （LPS）stimulated cultures of macrophages cells exposed to 10, 50, 100, and 200 mglmL for 24 h. The antiobesity activity of the extracts was deter mined as triglyceride content and by a lipolysis as say in differentiated 3T3LI cells exposed to the extracts for 72 h at the same concentrations de scribed above. RESULTS： All extracts showed similar antiprolifera tive activity in a dose and timedependent man ner in HS578T cells. Although extracts at lower con centrations and shorter times stimulated growth of T47D cells, the antiproliferative effects of the extracts on T47D cells at higher concentrations （〉100 mg/ mL） for 72 h were significantly greater than those of HS578T cells. In case of antiinflammatory activi ty, some extracts （AST, ASE, ACA, and AKE） signifi cantly reduced nitric oxide production at higher concentrations in the presence of LPS compared with that in control cells. Antiobesity activity was showed with reducing lipid accumulation signifi cantly （〉50%） at concentrations above 100 mg/mL in most extracts （except AST and ACA）. Additional ly, AKE and ASC increased lipolysis by 11%24% compared with that in the control. CONCLUSION： Artemisia spp. demonstrates poten tial as bioactive food supplements.展开更多
基金Supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Education, Science and Technology(2012-0006811)
文摘OBJECTIVE： To investigate the anticancer, antiin flammatory, and antiobesity activity of methanol extracts of eight distinct species： Artemisia Stolon ifera （AST）, Artemisia Selengensis （ASE）, Artemisia .la ponica, Artemisia Montana, Artemisia Capillaris （ACA）, Artemisia Sylvatica （ASY）, Artemisio Keiskeana （AKE）, and Artemisia Scoparia （ASC） in vitro. METHODS： Antiproliferative activity was investigat ed in human breast cancer estrogen receptora pos itive T47D and negative HS578T cell lines exposed to the extracts at various concentrations （5200 mgl mL）for24, 48, and 72 h. For evaluating the antiin flammatory activity of the extracts, inhibition of ni trite synthesis was investigated in lipopolysaccha ride （LPS）stimulated cultures of macrophages cells exposed to 10, 50, 100, and 200 mglmL for 24 h. The antiobesity activity of the extracts was deter mined as triglyceride content and by a lipolysis as say in differentiated 3T3LI cells exposed to the extracts for 72 h at the same concentrations de scribed above. RESULTS： All extracts showed similar antiprolifera tive activity in a dose and timedependent man ner in HS578T cells. Although extracts at lower con centrations and shorter times stimulated growth of T47D cells, the antiproliferative effects of the extracts on T47D cells at higher concentrations （〉100 mg/ mL） for 72 h were significantly greater than those of HS578T cells. In case of antiinflammatory activi ty, some extracts （AST, ASE, ACA, and AKE） signifi cantly reduced nitric oxide production at higher concentrations in the presence of LPS compared with that in control cells. Antiobesity activity was showed with reducing lipid accumulation signifi cantly （〉50%） at concentrations above 100 mg/mL in most extracts （except AST and ACA）. Additional ly, AKE and ASC increased lipolysis by 11%24% compared with that in the control. CONCLUSION： Artemisia spp. demonstrates poten tial as bioactive food supplements.
