Testing the parts of mechanical products and ensuring their accuracy to the design requirements are essential to products’ quality, market competitiveness and manufacturers’ maximum economical benefits from these pr...Testing the parts of mechanical products and ensuring their accuracy to the design requirements are essential to products’ quality, market competitiveness and manufacturers’ maximum economical benefits from these products. One of the latest subjects of study in the area of precision measurement is the testing of parts to follow the relative requirements, viz. design requirements for the size tolerance of size features and related geometrical tolerances of the central feature, including the envelope requirement, maximum material requirement and least material requirement. The article analyzes test methods for parts to follow the envelope requirement or maximum material requirement, as well as further requirements of geometrical tolerances for its central feature. The method is effective in improving product quality and rejecting unqualified parts.展开更多
[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
Four rapid spectral comparison methods are introduced in mobile labs.They are conformity test method,general correlation coefficient method,reverse correlation coefficient method and correlation coefficient method usi...Four rapid spectral comparison methods are introduced in mobile labs.They are conformity test method,general correlation coefficient method,reverse correlation coefficient method and correlation coefficient method using characteristic spectral ranges.The first method is used for tracking the movements of drugs in the distribution channels;the second is used for quickly identify new counterfeit drugs;the last two are used to screen drugs illegally added in Traditional Chinese Medicine （TCM）.The applicability of the four methods is evaluated with counterfeit and authentic drugs.Our results show that these methods can be quickly constructed and used to identify counterfeit drugs accurately.展开更多
文摘Testing the parts of mechanical products and ensuring their accuracy to the design requirements are essential to products’ quality, market competitiveness and manufacturers’ maximum economical benefits from these products. One of the latest subjects of study in the area of precision measurement is the testing of parts to follow the relative requirements, viz. design requirements for the size tolerance of size features and related geometrical tolerances of the central feature, including the envelope requirement, maximum material requirement and least material requirement. The article analyzes test methods for parts to follow the envelope requirement or maximum material requirement, as well as further requirements of geometrical tolerances for its central feature. The method is effective in improving product quality and rejecting unqualified parts.
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
基金National Key Technologies R&D Program Foundation of China(Grant No.2008BAI55B06).
文摘Four rapid spectral comparison methods are introduced in mobile labs.They are conformity test method,general correlation coefficient method,reverse correlation coefficient method and correlation coefficient method using characteristic spectral ranges.The first method is used for tracking the movements of drugs in the distribution channels;the second is used for quickly identify new counterfeit drugs;the last two are used to screen drugs illegally added in Traditional Chinese Medicine （TCM）.The applicability of the four methods is evaluated with counterfeit and authentic drugs.Our results show that these methods can be quickly constructed and used to identify counterfeit drugs accurately.
