Many analytic strategies have emerged to estimate plant responses to Fusarium wilt. The demand for fast and reliable method （diagnosis, prediction） to determine isolate strength accurately is not established yet. Ea...Many analytic strategies have emerged to estimate plant responses to Fusarium wilt. The demand for fast and reliable method （diagnosis, prediction） to determine isolate strength accurately is not established yet. Early determination of pathogen strength helps in plant medication. The aim of this study was to develop a faster strategy and method for early determination of fungal isolates strength in correlation to plant response. Till now, the scientists have no consensus on the most correlated parameters that could express wilt precisely. In this study, 30 isolates of Fusarium oxysporum isolated from Lupinus termis L. were used to provide an explicit image about the real strength of Fusarium isolates and its impact on the plant. Wilting percentage ranged from 26.67% to 93.33% of the infected plants depending on isolate virulence. Some of cellular, morphological and physical measurements were conducted on 8 out of 30 isolates, including root （length, fresh weight （FW） and dry weight （DW））, nodules （water content （WC）, FW, DW）, stem （height, WC, FW, DW）, total leaves/plant （WC, FW, DW） and the fourth leaf （WC, FW, DW, leaf area, epidermal cell area, epidermal cell number, succulence）. Hierarchical clustering was used to determine the variance between the isolates. Detrended correspondence analysis （DCA） and canonical correspondence analysis （CCA） were used to determine the most important growth parameters that could express wilting accurately. The CCA results showed that most of the measured parameters on the fourth leaf, except for leaf epidermal cell number, were highly and positively correlated to wilt. That makes these specific parameters valuable and sensitive for any changes in isolates strength. Accordingly, a mathematical model was created to be helpful in the quick determination of isolate strength and precise medication.展开更多
Drought can affect the growth and soil enzyme activities of invasive alien plants(IAPs).It is imperative to evaluate the competitive advantage of IAPs compared with that of the native species and the activities of soi...Drought can affect the growth and soil enzyme activities of invasive alien plants(IAPs).It is imperative to evaluate the competitive advantage of IAPs compared with that of the native species and the activities of soil enzymes under drought.This study aimed to evaluate the competitive advantage of the IAP Amaranthus spinosus that originated from tropical America compared with the native Chinese species A.tricolor and the activities of soil enzymes under drought.A competitive co-culture of A.spinosus and A.tricolor was established using a planting basin experiment.The two species were treated with different levels of drought,i.e.(i)the control;(ii)a light level of drought and(iii)a heavy level of drought.The functional traits,osmotic adjustment and the activities of antioxidant enzymes of the two species,as well as soil pH and electrical conductivity,contents of soil microbial biomass carbon and the activities of soil enzymes were determined.The relative competition intensity and relative dominance of A.spinosus were greater than those of A.tricolor under drought.Drought may provide an advantage to the competitive advantage of A.spinosus.Soil water-soluble salt content and sucrose hydrolytic power of A.spinosus were greater than those of A.tricolor under drought.The ability of A.spinosus to grow in soil with higher levels of water-soluble salt contents and sucrose hydrolytic power under drought may aid in its acquisition and utilization of nutrients.展开更多
文摘Many analytic strategies have emerged to estimate plant responses to Fusarium wilt. The demand for fast and reliable method （diagnosis, prediction） to determine isolate strength accurately is not established yet. Early determination of pathogen strength helps in plant medication. The aim of this study was to develop a faster strategy and method for early determination of fungal isolates strength in correlation to plant response. Till now, the scientists have no consensus on the most correlated parameters that could express wilt precisely. In this study, 30 isolates of Fusarium oxysporum isolated from Lupinus termis L. were used to provide an explicit image about the real strength of Fusarium isolates and its impact on the plant. Wilting percentage ranged from 26.67% to 93.33% of the infected plants depending on isolate virulence. Some of cellular, morphological and physical measurements were conducted on 8 out of 30 isolates, including root （length, fresh weight （FW） and dry weight （DW））, nodules （water content （WC）, FW, DW）, stem （height, WC, FW, DW）, total leaves/plant （WC, FW, DW） and the fourth leaf （WC, FW, DW, leaf area, epidermal cell area, epidermal cell number, succulence）. Hierarchical clustering was used to determine the variance between the isolates. Detrended correspondence analysis （DCA） and canonical correspondence analysis （CCA） were used to determine the most important growth parameters that could express wilting accurately. The CCA results showed that most of the measured parameters on the fourth leaf, except for leaf epidermal cell number, were highly and positively correlated to wilt. That makes these specific parameters valuable and sensitive for any changes in isolates strength. Accordingly, a mathematical model was created to be helpful in the quick determination of isolate strength and precise medication.
基金This study was funded by Opening Project of State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,China(K2020205)National Natural Science Foundation of China(32071521)+1 种基金Key Research and Development Program of Changzhou,China(CJ20200013)Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment.
文摘Drought can affect the growth and soil enzyme activities of invasive alien plants(IAPs).It is imperative to evaluate the competitive advantage of IAPs compared with that of the native species and the activities of soil enzymes under drought.This study aimed to evaluate the competitive advantage of the IAP Amaranthus spinosus that originated from tropical America compared with the native Chinese species A.tricolor and the activities of soil enzymes under drought.A competitive co-culture of A.spinosus and A.tricolor was established using a planting basin experiment.The two species were treated with different levels of drought,i.e.(i)the control;(ii)a light level of drought and(iii)a heavy level of drought.The functional traits,osmotic adjustment and the activities of antioxidant enzymes of the two species,as well as soil pH and electrical conductivity,contents of soil microbial biomass carbon and the activities of soil enzymes were determined.The relative competition intensity and relative dominance of A.spinosus were greater than those of A.tricolor under drought.Drought may provide an advantage to the competitive advantage of A.spinosus.Soil water-soluble salt content and sucrose hydrolytic power of A.spinosus were greater than those of A.tricolor under drought.The ability of A.spinosus to grow in soil with higher levels of water-soluble salt contents and sucrose hydrolytic power under drought may aid in its acquisition and utilization of nutrients.
