Recycling of metals from waste Sn-based alloys by vacuum separation

In order to recycle waste Sn-based alloys, the vapor-liquid phase equilibrium composition diagrams of Sn-Pb, Sn-Sb and Sn-Zn binary systems were calculated. The calculated results indicate that Pb, Sb and Zn can be separated from Sn effectively. Based on the above calculation, the industrial experiments of vacuum distillation of Sn-Pb alloy, Sn-Pb-Sb alloy, Sn-Pb-Sb-As alloy, crude Sn and Sn-Zn alloy with different contents were carried out. The experimental results show that Pb(>99% Pb) and Sn(≤0.003% Pb) were obtained simultaneously while Sn-Pb alloy was subjected to vacuum distillation; the crude Sn(>90% Sn, ≤ 2% Pb, ≤6% Sb) and crude Pb(≤2% Sn) were obtained simultaneously while a single vacuum distillation was carried out for Sn-Pb-Sb alloy; the Pb and Bi contents in the Sn ingot(99.99% Sn) achieve the grade A of GB/T 728—2010 standard, more than 50% of As and Sb was removed after vacuum distillation of crude Sn; Zn(<0.002% Sn) and Sn(about 3% Zn) were obtained while vacuum distillation of Sn-Zn alloy was conducted at 1173 K, 20-30 Pa for 8-10 h.

Synthesis of a New Series of Chalcone Derivatives and Their Antifungal Activities

To study the antifungal effect of chalcone derivatives. Methods Sixteenchalcone derivatives were synthesized and confirmed by ~1H NMR and IR spectra, and tested forantifungal activity against four common pathogenic fungi. Their structure-activity relationship isdiscussed. Results Among 16 title compounds, there were 5 new compounds, which have not beenreported before. The preliminary antifungal test showed that all title compounds exhibitedantifungal activities to a certain extent. The activity of compound 8 against Trichophyton rubrumhad a potency equal to that of fluconazole, with a MIC of 4 μg·mL^(-1) . Conclusion Sixteenchalcones were prepared and their antifungal activities against four common pathogenic fungi invitro were examined. Some of them exhibited antifungal activities to a certain extent.

生活作文贵求真

生活作文，贵求一个“真”字。如何达到“真”？必须做到无拘无柬说“真话”，有条有理叙“真事”，细致入微吐“真情”，最终“千学万学学做真人”。

Synthesis and Antifungal Activity of 1-(1H-1,2,4-Triazole)-2-(2,4-diflurophenyl)-3-(N-methyl-N-substituted benzylamino)-2-propanols

Eleven 1-(1H-1,2,4-triazole)-2-(2,4-diflurophenyl)-3-(N-methyl-N-substituted benzylamino)-2-propanols were designed and synthesized, on the basis of the crystal structure of P450 cytochrome 14α-sterol demethylase(CYP51) and the docking results of inhibitors to the active site of the enzyme. All title compounds were first by reported. Results of preliminary biological tests showed that most of title compounds exhibited activity against the seven common pathogenic fungi. Compound 11 showed best antifungal activity with broad antifungal spectrum and proved to be more active against Cryptococcus neoformans, Candida albicans, Microsporum lanosum and Trichophyton rubrum than ketoconazole. Compounds 3, 10 and 4 also had high activities.

真情融入作文里

就目前来看,很多时候学生写作文时都是违心而作,没有情感的参与。本文简单介绍了笔者经过实践总结出的一些有效的策略：通过＂降低标准,学会关注生活,减少思想束缚,善待雏形习作＂等策略来实现学生作文＂言之有物＂,写出真情实感,将真情融入学生习作。

On Naipaurs novels from reality and acuity

Naipaul＇s novels are real and acute, which reflect many countries and social realities, especially terrible lives and depressed emotions, including human interests, opinions, ideas, consciousnesses, self-acceptance, idea crisis, looking for identity, spiritual trouble, contradiction between religion and life, cultural conflict in colonized countries and post-colonized areas. Such novels are from actual realities, life facts, street matters with miscellaneous, vicissitudinous, and integrative skills and abilities.

Antifungal Activity of Lactic Acid Bacteria, Isolated from Bulgarian Wheat and Rye Flour

The economic losses and the health hazards of the mycotoxins produced by spoilage fungi are the main concerns of the food industry. The spoilage of bakery products by fungi is more common in countries with a high humidity and temperature. About 5-10% of food production is spoiled by the growth of yeast and fungi in food materials. Similarly, in Western Europe, the growth of the spoilage fungi of bread is estimated to reach more than 200 million Euros per year. The history conditions of the food can be a major factor in determining any fungal spoilage--for example, stored and processed foods are more sensitive to spoilage when compared with fresh and prepared foods. Lactic acid bacteria isolated from Bulgarian wheat and rye flour were used in the present study to check their antifungal properties against pathogenic yeast and fungi imperfecta using standard disc diffusion method in vitro. A broad spectrum of antifungal activity of the six newly identified as L. plantarum strains e Tsl, Ts2, Ts3,Ts4 and Ts5, and L. helveticus Ts6 was estimated. Our in vitro studies were performed with wheat and rye sourdough, in order to simulate a real product and to assess the bio-protective potential of the tested lactobacilli. The used test-cultures are representatives of carcinogenic, toxigenic, deteriorative and allergenic fungi from the genera .4spergillus and Penicillium. The all tested strains completely suppress the growth of against C. glabrata 72. Strains L. plantarum Tsl and Ts3 completely suppress the growth against S. cerevisae. While, in the sample with L. plantarum strains e Tsl, Ts2, Ts3,Ts4 and Ts5, and L. helveticus Ts6, a retarded and weak growth of A. niger and P. claviforme was observed. However, the spore germination and the colony growth started only on the fifth day of the mould lactobacilli co-cultivation, which also should be considered as a good result. In this study six isolates Tsl,Ts2, Ts3, Ts4, Ts5 and Ts6, from the traditional Bulgarian wheat and rye flour have been identified as L. plantarum and L. helveticus and characterized as cultures with promising antifungal activity. Obtained results from the combined molecular identification （16S rRNA gene sequencing） approach contribute to give new data on the microbial biodiversity of this not well-studied niche. The antifungal activity of our new isolates, identified as L. plantarum and L. helveticus, seems to be a promising advantage of these six strains, suggesting their potential applications in different food technologies. However, more experiments have to be conducted to clarify the nature and the mechanisms of the reported antifungal activity and they are still in progress. The combination of dairy origin and strong inhibitory activity of the lactobacillus strains is a prerequisite for their possible application as starters and/or bioprotective antifungal adjuncts.

Cloning and Efficient Expression of Bacillus sp. BH072 tas A Gene in Escherichia coli

The Bacillus strain BH072 isolated from a honey sample showed strong antifungal activity against phytopathogen. Gene cloning test demonstrated that the strain had a tasA gene encoding an antifungal TasA protein. Although the wild strain simultaneously produced various antifungal substances, only the physicochemical property and antifungal activity of TasA protein were unclear due to the difficulty in extraction. In this study, tasA gene encoding the protein from Bacillus sp. BH072 was amplified by using the polymerase chain reaction （PCR） method and cloned into pET 28a （＋） vector, and then expressed in host cells Escherichia coli BL21 （DE3）. The expressed proteins were collected by centrifugation and ultrasonic treatment, and then purified by using nickel-nitrilotriacetic acid （Ni-NTA） metal affinity column and dialysis methods. The result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） test showed that an expected protein band appeared with a size of 31 kDa. The expressed products possessed antifungal activity against the phytopathogenic indicator strain Botrytis cinerea. A genetically engineered strain tasA orE, coli was established in this study which can efficiently express Tas A protein.

Antitumor and Antifungal Activities of Organic Extracts of Seacucumber Holothuria atra from the Southeast Coast of India

In phylum Echinodermata, the family Holothuridae is distinguished by its capacity of bioactive compounds. Sea cu- cumber Holothuria atra is commonly known as the lollyfish. The antifimgal activity was detected using agar well diffusion method against the various fungal strains such as Trichoderma viride, Aspergillus niger, Aspergillus flavis, Candida albicans and Penicillium chrysogenum. Relatively high antifungal activity was seen against Candida albicans at 100 μL-1 concentration of extracts. Zone of inhibition was measured at 18 mm of diameter. The anti-tumor activities were detected against the Vero and Hep2 cell lines using MTT assay. The cells were treated with H. atra extract at concentrations 0.078-10mg mL-1. The extract showed high proliferative activity against the Hep2 cells. The body wall extracts of sea cucumber （H. atra） showed effective antifungal and antitumor activities All these findings suggest that the extracts could be used for the development of drugs.

Utility of an upright-type 11-gauge stereotactic vacuum-assisted biopsy device (Mammotome) for the diagnosis of breast microcalcifications

Objective: The aim of this study was to evaluate the utility of an upright-type 11-gauge stereotactic vacuumassisted biopsy device (Mammotome) for the diagnosis of breast microcalcifications Methods: Between May 2001 and October 2005, 154 biopsies in 152 patients with microcalcifications were performed using the upright-type 11-gauge stereotactic vacuum-assisted biopsy device. Patients in whom this biopsy was diagnosed as carcinoma or a borderline lesion, had a subsequent surgical excision of the lesion. Histopathological and radiological features of the two specimens were then compared with each other. Results: Microcalcification was identified on specimen mammograms and microscopic slides in 97.4% of cases. Of 154 Mammotome biopsies 98 (63.6%) were benign, 51 (33.1%) were malignant, 3 (1.9%) showed atypical hyperplasia, and 2 (1.3%) were indeterminate, respectively. Of the 48 cases that received surgical excision, 6 of 36 ductal carcinomas in situ (16.7%) upstaged to invasive ductal carcinoma and 1 of 2 atypical ductal hyperplasias was upstaged to ductal carcinoma in situ. The positive predictive value of the 11-gauge Mammotome for the diagnosis of invasion in breast cancer was 100%. Linear calcification and pleomorphic calcification linear/segmental distribution was reliable indications of malignancy. The mean follow-up time of the benign lesions was 22 months, and without evidence of lesion growth. Complications included vasovagal reactions (6.3%), bleeding (0.6%) and hematoma (2.6%). Conclusion: The upright stereotactic 11-gauge Mammotome procedure is an effective and reliable method for the diagnosis of breast microcalcifications. It has minimal side effects. For lesions diagnosed as ADH or DCIS with the 11-gauge Mammotome, subsequent surgical excision should be performed.

Characteristics of activated carbon prepared from Chinese fir sawdust by zinc chloride activation under vacuum condition

The preparation of activated carbon from Chinese fir sawdust by zinc chloride activation under both nitrogen atmosphere and vacuum conditions was carded out in a self-manufactured vacuum pyrolysis reactor. The effects of the system pressure and the activation condition （nitrogen or vacuum） on pore development were investigated. The results show that both high quality activated carbon and high added-value bio-oil can be obtained simultaneously via vacuum chemical activation. The characteristics of the activated carbons produced under vacuum conditions are better than those prepared under nitrogen atmosphere. The performance parameters of the activated carbon obtained under vacuum conditions are as follows： the pore size distribution is mainly microporous, the Brunauer-Emmett-Teller （BET） surface area is 1 070.59 m^2/g, the microporous volume is 0.502 4 cm^3/g, the average pore size is 2.085 nm, and the iodine adsorption value and the methylene blue adsorption value are 1 142.92 and 131.34 mg/g, respectively. The activated carbon from vacuum chemical activation has developed micropores, and the N2 adsorption equilibrium constant of the corresponding activated carbon gradually increases with the decrease of reaction system pressure.

Binding activity of H-Ras is necessary for in vivo inhibition of ASK1 activity

H-Ras is well known as one of the essential components of Ras/Raf/MEK/ERK cascade, which is a critical prosurvival signaling mechanism in most eukaryotic cells. Ras targets Raf/MEK/ERK cascade by integrating and transmitting extracellular signals from growth factor receptors to Raf, leading to the propagation of signals to modulate a serious of cellular survival events. Apoptosis signal-regulating kinasel (ASK1) serves as a general mediator of cell death because it is responsive to a variety of death signals. In this study, we found that H-Ras interacted with ASK1 to cause the inhibition of both ASK1 activity and ASK1-induced apoptosis in vivo, which was reversed only partially by addition of RafS621A, an antagonist of Raf, whereas MEK inhibitor, PD98059, and PI3K inhibitor, LY294002, did not disturb the inhibitory effect of H-Ras on ASK-1-induced apoptosis. Furthermore, by means of immunoprecipitate and kinase assays, we demonstrated that the interaction between H-Ras and ASK1 as well as the inhibition of ASK1 activity were dependent on the binding activity of H-Ras. These results suggest that a novel mechanism may be involved in H-Rasmediated cell survival in addition to the well established MEK/ERK and PI3K/Akt kinase-dependent enhancement of cell survival.

OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1

WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways. It has been shown that one Arabidopsis WRKY protein, AtWRKY29/22, is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens. However, little is known about the biological roles of WRKY proteins in rice. In this study, we investigated the expression patterns of rice AtWRKY29/22 homolog, OsWRKY03, under different conditions, and also its possible role involved in plant defense. Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments. Further analysis revealed that the expression of OsWRKY03 was light dependent. Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay. Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein. OsNPR1 as well as several other pathogenesis-related genes, such as OsPRlb, phenylalanine ammonia-lyase （ZB8） and peroxidase （POX22.3）, were induced in OsWRKYO3-overexpressing transgenic plants. These results indicated that OsWRKY03 is located upstream of OsNPR 1 as a transcriptional activator in salicylic acid （SA）-dependent or jasmonic acid （JA）-dependent defense signaling cascades.

Isolation and Identification of Cardenolide Compounds of Gomphocarpus sinaicus and Their Fungicidal Activity Against Soil Borne and Post Harvest Fungi

This study was undertaken to explore new antifungal compounds from the methanolic extract of G. sinaicus. Two cardenolide compounds were isolated and identified by GC-MS as cardenolide glycoside, 15-hydroxy-3,4,5,6-dehydrocalotropin and cardenolide genin, 3,4,5,6-dehydrocalotropagenin. The antifungal activity of these compounds was assessed. Results revealed that both compounds showed pronounced fungicidal activity against both soil borne fungi, R. solani, F. oxysporium, and postharvest fungi, R. stolonifer, P. digtatum, compared to the standard fungicides, flutolanil and copper oxychloride, respectively. The ECs0 values of the cardenolide genin were 0.703, 13.63 and 4.22, 8.403 lag/mL forR. solani, F. oxysporium andR. stolonifer, P. digtatum respectively. On the other hand, the ECs0 values of the standard fungicide, flutolanil, were 9.49 and 61.22 ~tg/mL against R. solani and F. oxysporium. While the ECso values of copper oxychloride were 279.94 and 187.13 p.g/mL against R. stolonifer and P. digtatum, respectively. The results showed that cellulase, PME, PPO of the tested fungi was more sensitive than to cardenolide genin. The strong antifungal activity of cardenolide genin reported in this study indicated that has a potential to be used as fungicides.

Synthesis and antifungal activity of 1-(1H-1,2,4-triazol-1-yl)-2-(2,4-difluorophenyl)-3-(4-substituted acyl piperazin-1-yl)-2-propanols

Objective: To study the effect of fluconzole derivatives from a side chain containing 4-substituted acyl piperazin-1-yl on antifungal activity. Methods: Fourteen title compounds were synthesized and confirmed by the elementary analysis, 1HNMR and IR spectra. Five deep fungal strains and 3 shallow fungal strains were chosen as the experimental strains.Minimum inhibitory concentrations(MICs) of all title compounds were determined by the method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) using RPMI 1640 test medium. Results: Among the 14 title compounds, 12 were first reported. The results of preliminary antifungal test showed that all the title compounds exhibited potent antifungal activities to a certain extent. The activity of 4 compounds were more than 4 times as high as that of fluconazole and equal to that of ketoconazole against Candida albicans in vitro(MIC 80 value≤0.125 μg/ml). Conclusion: Introduction of a side chain containing 4-substituted acyl piperazin-1-yl into the main part of fluconazole has important influence on antifungal activities of title compounds.

In Vitro Activity of Lawsonia inermis （Henna） on Some Pathogenic Fungi

The present study was conducted to investigate antifungal activity ofLawsonia inermis （Henna plant）. Leaf samples of the plants were collected from Eastern Nile of Khartoum state, Sudan. Ethanol and petroleum ether extracts in various concentrations were obtained by maceration （cold method）. The extracts were bioassay in vitro to know their bioactivity to inhibit the growth of tested fungi. The cup plate agar diffusion method was adopted to assess the antifungal activity of the extracts against tested yeasts while agar incorporated method was used for other molds. Both extracts revealed anti fungal activity against all yeast strains except Pichiafabianii which was found resistant to both ethanol and ether extracts. The results displayed antifungal activity against tested fungi. Minimum mould concentration （MMC） of the extracts was determined. The obtained results revealed antifungal activity of henna leaves extracts which support the traditional use of the Henna in therapy of fungal infections. The possibility of therapeutic use of Sudanese henna as antifungal agents is recommended.

Synthesis and Biological Activity of N-Heteroaromatic- substituted Thiophene-2-Carbohydrazides

New heteroring-substituted thiophene-2-carbohydrazides incorporating pyrrole, thiophene, and pyridine moieties have been synthesized, characterized, and screened for possible antimicrobial activity. Some of the synthesized compounds exhibited promising antibacterial activities, but rather modest antifungal activity.

Expression, Characterization and Antimicrobial Ability of a Variant T4 Lysozyme in Pichia pastoris

T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophiliza-tion. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cel wal of Xan-thomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb. nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. mal-vacearum, Fusarium oxysporium sp. vasinfectum, Verticil ium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasin-fectum and V. d. kleb were also analyzed.

Scoping Antifungal Activities of Various Forms of Chitosan Oligomers and Their Potential Fixation in Wood

Chitosan oligomers （average Dp-4） are known for their antifungal activity and wood decay resistance. These oligomers are susceptible to moisture, and promote yeast growth upon air exposure after a sufficient length of time. Chitosan oligomers of three different states viz. completely dried, freshly prepared and highly viscous form, were prepared to compare their in-vitro antifungal activities against three brown-rot fungi, two sapstain and one mould fungus using agar nutrient medium. Additionally, a mixture of chitosan oligomers and boric acid was used for wood treatment. The nutrient medium bioassay results show that all states of chitosan oligomers inhibited the growth of tested basidiomycetes fungi, but not sapstain and mould fungi. Subsequently, wood decay results confirm antifungal activity of chitosan oligomers plus boron against basidiomycetes, but highlighted their leachability upon water exposure.

Isolation and Antifungal Evaluation of Rumex cyprius Murb Extracts

Ethanolic extract of dried Rumex cyprius Murb （polygonaceae） was tested against four pathogenic fungi： three dermatophytes, Microsporum canis, Trichophyton mentagrophytes and T. rubrum, and the causative agent of chalkbrood disease of bees, Ascophaera apis. The extract showed considerable activity against all these fungi. One of the main constituents ofRumex cyprius was isolated and tested for its antimycotic activity. It showed a significant activity against the test fungi and was identified as 1,3,8-trihydroxy-6-methylanthracene-9,10-dione based on its IR, UV-V and 1^H NMR spectra.