A set of three sphingolipids, N-2′-hydroxyplmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4E, 8E-sphingadiene （A）, N-2′-hydroxyl-3′E-octadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4E, 8E-sphingediene （B） and N-palmito...A set of three sphingolipids, N-2′-hydroxyplmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4E, 8E-sphingadiene （A）, N-2′-hydroxyl-3′E-octadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4E, 8E-sphingediene （B） and N-palmitoyldihydrosphingosine （C）, were isolated from two marine-derived mangrove endophytic fungal strains （strains No. 1924 and 3893） from the South China Sea. Their structures were elucidated by 2D NMR and FABMS methods. It is the first time that these sphingolipids were separated and obtained from marine-derived mangrove endophytic fungus from the South China Sea.展开更多
Two metabolites （A and B） were isolated from the mycelium of mangrove endophytic fungus Stysanus like sp. （#2492） from the South China Sea. Their structures were identified by spectral data as N-（2-hydroxytetraco...Two metabolites （A and B） were isolated from the mycelium of mangrove endophytic fungus Stysanus like sp. （#2492） from the South China Sea. Their structures were identified by spectral data as N-（2-hydroxytetracosyl）-2-amino-1,3,4-trihydroxyoctadecane （A） and γ -stearolactone （B）. It is the first report that γ -stearolactone （B） is isolated from marine fungus as natural product.展开更多
[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantle...[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.展开更多
Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), a...Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).展开更多
In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W(1a), as well as five known diketopiperazine alk...In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W(1a), as well as five known diketopiperazine alkaloids, diketopiperazine V(1b), brevianamide Q(2), brevianamide R(3), brevianamide K(4), and brevianamide E(5), were isolated from the Et OAc extract of the fermentation broth. Their structures were elucidated by spectroscopy techniques(NMR, MS). The six compounds exhibited moderate radical scavenging activity against DPPH with clearance ratio of 55.0%(1a and 1b), 53.7%(2), 46.2%(3), 61.4%(4) and 19.3%(5) at a concentration of 13.9 μmol L-1, respectively; while the positive control ascorbic acid showed a ratio of 70.3% at the concentration of 28.4 μmol L-1.展开更多
This review describes the present hot research areas of mangrove-associated fungi, including its biodiversity, ecological roles, novel metabolites productions and biotechnological potential. Mangrove-associated fungi ...This review describes the present hot research areas of mangrove-associated fungi, including its biodiversity, ecological roles, novel metabolites productions and biotechnological potential. Mangrove-associated fungi were divided into saprophytic, parasitic and true symbiotic fungi based on its ecological roles. Saprophytic fungi are fundamental to decomposition and energy flow of mangrove, additionally, their potential toxicity also exists. Pathogenic fungi have significant effects on mangrove survival, growth, and fitness. Endophytic fungi, the most prolific source of diverse bioactive compounds found among that of mangrove-associated fungi, are found in most species of mangroves. Although a significant number of reports focused on the antimicrobial, insecticidal and other bioactive metabolites as well as many novel enzymes isolatcd from mangrove-associated fungi, and many of those metabolites from endophytic fungi are suspected to be of significant to mangrove, only few studies have provided convincing evidence for symbiotic producers in mangrove. Hence, this paper discusses the present progress of molecular methods used to correlate the ecological roles of endophytic fungi with their bioactive metabolites;, meanwhile, the potential of using metabolic engineering and post-genomic approaches to isolate more novel enzymes and bioactive compounds and to make their possible commercial application was also discussed.展开更多
Seaweeds are known to exhibit various antimicrobial properties, since it harbours an enormous range of indigenous bioactive compounds. The emergence of drug resistant strains has directed to the identification of pros...Seaweeds are known to exhibit various antimicrobial properties, since it harbours an enormous range of indigenous bioactive compounds. The emergence of drug resistant strains has directed to the identification of prospective metabolites from seaweed and its endophytes, thereby exploiting the properties in resisting bacterial diseases. The current study was aimed to assess the antimicrobial activity of extracts obtained from Ulva reticulate, for which metabolites of Ulva reticulata and its endophytes were extracted and assessed against human pathogens like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Bacillus subtilis. It was observed that the hexane extract of isolate VITDSJ2 was effective against all the tested pathogens but a significant inhibition was observed for Staphylococcus aureus and Escherichia coli. Further, Gas chromatography coupled with Mass spectroscopy （GC-MS） revealed the existence of phenol, 3, 5-bis （1, 1-dimethylethyl） in the crude hexane extract which is well-known to possess antibacterial activity. The effective isolate VITDSJ2 was identified to be the closest neighbour of Pseudomonas smtzeri by phenotypic and genotypic methods. The crude extracts of the seaweed Ulva reticulata was also screened for antibacterial activity and the hexane extract was effective in showing inhibition against all the tested pathogens. The compound in the crude extract of Ulva reticulata was identified as hentriacontane using GC-MS. The extracts obtained from dichloromethane did not show significant activity in comparison with the hexane extracts. Hence the metabolites of Ulva reticulata and the bacterial secondary metabolites of the endophytes could be used in the treatment of bacterial infections.展开更多
基金the National ＂863＂ Program of China （No. 2001AA624010）the Guangdong Provincial Natural Science Foundation of China （No. 04300674）+1 种基金 the Guangdong Provincial Science and Technology Program Foundation of China （No.2004B30101017） the Foshan City Foundation for the Development of Science and Technology （No. 2005081871）.
文摘A set of three sphingolipids, N-2′-hydroxyplmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4E, 8E-sphingadiene （A）, N-2′-hydroxyl-3′E-octadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4E, 8E-sphingediene （B） and N-palmitoyldihydrosphingosine （C）, were isolated from two marine-derived mangrove endophytic fungal strains （strains No. 1924 and 3893） from the South China Sea. Their structures were elucidated by 2D NMR and FABMS methods. It is the first time that these sphingolipids were separated and obtained from marine-derived mangrove endophytic fungus from the South China Sea.
文摘Two metabolites （A and B） were isolated from the mycelium of mangrove endophytic fungus Stysanus like sp. （#2492） from the South China Sea. Their structures were identified by spectral data as N-（2-hydroxytetracosyl）-2-amino-1,3,4-trihydroxyoctadecane （A） and γ -stearolactone （B）. It is the first report that γ -stearolactone （B） is isolated from marine fungus as natural product.
基金Supported by the National Natural Science Foundation of China(31070443,30970523)the National Science Foundation for Talents Training in Basic Science,China(J1103507)the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions,China~~
文摘[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.
基金Supported by the Ministry of Science and Technology(No.2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-G-12B)the National Natural Science Foundation of China(No.30910103914)
文摘Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).
基金supported by Chinese National Science Fund (No. 41176120)Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. BS2010HZ027)+1 种基金Specialized Research Fund for the Doctoral Program of Higher Education (No. 20100132120026)the Fifty First Postdoctoral Fund of China (2012M511552)
文摘In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W(1a), as well as five known diketopiperazine alkaloids, diketopiperazine V(1b), brevianamide Q(2), brevianamide R(3), brevianamide K(4), and brevianamide E(5), were isolated from the Et OAc extract of the fermentation broth. Their structures were elucidated by spectroscopy techniques(NMR, MS). The six compounds exhibited moderate radical scavenging activity against DPPH with clearance ratio of 55.0%(1a and 1b), 53.7%(2), 46.2%(3), 61.4%(4) and 19.3%(5) at a concentration of 13.9 μmol L-1, respectively; while the positive control ascorbic acid showed a ratio of 70.3% at the concentration of 28.4 μmol L-1.
基金Foundation project： This work was supported partly by the Guangzhou Natural Science Foundation （Grant No. 2007Z3-EO581）, the Guangdong Provincial Natural Science Foundation （Grant No. 2007A0200300001-7 05003268）, the Chinese High-Tech 863 Project （Grant No. 2006AA09Z422）, and the National Natural Science Foundation of China （Grant No. 20572136）.
文摘This review describes the present hot research areas of mangrove-associated fungi, including its biodiversity, ecological roles, novel metabolites productions and biotechnological potential. Mangrove-associated fungi were divided into saprophytic, parasitic and true symbiotic fungi based on its ecological roles. Saprophytic fungi are fundamental to decomposition and energy flow of mangrove, additionally, their potential toxicity also exists. Pathogenic fungi have significant effects on mangrove survival, growth, and fitness. Endophytic fungi, the most prolific source of diverse bioactive compounds found among that of mangrove-associated fungi, are found in most species of mangroves. Although a significant number of reports focused on the antimicrobial, insecticidal and other bioactive metabolites as well as many novel enzymes isolatcd from mangrove-associated fungi, and many of those metabolites from endophytic fungi are suspected to be of significant to mangrove, only few studies have provided convincing evidence for symbiotic producers in mangrove. Hence, this paper discusses the present progress of molecular methods used to correlate the ecological roles of endophytic fungi with their bioactive metabolites;, meanwhile, the potential of using metabolic engineering and post-genomic approaches to isolate more novel enzymes and bioactive compounds and to make their possible commercial application was also discussed.
文摘Seaweeds are known to exhibit various antimicrobial properties, since it harbours an enormous range of indigenous bioactive compounds. The emergence of drug resistant strains has directed to the identification of prospective metabolites from seaweed and its endophytes, thereby exploiting the properties in resisting bacterial diseases. The current study was aimed to assess the antimicrobial activity of extracts obtained from Ulva reticulate, for which metabolites of Ulva reticulata and its endophytes were extracted and assessed against human pathogens like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Bacillus subtilis. It was observed that the hexane extract of isolate VITDSJ2 was effective against all the tested pathogens but a significant inhibition was observed for Staphylococcus aureus and Escherichia coli. Further, Gas chromatography coupled with Mass spectroscopy （GC-MS） revealed the existence of phenol, 3, 5-bis （1, 1-dimethylethyl） in the crude hexane extract which is well-known to possess antibacterial activity. The effective isolate VITDSJ2 was identified to be the closest neighbour of Pseudomonas smtzeri by phenotypic and genotypic methods. The crude extracts of the seaweed Ulva reticulata was also screened for antibacterial activity and the hexane extract was effective in showing inhibition against all the tested pathogens. The compound in the crude extract of Ulva reticulata was identified as hentriacontane using GC-MS. The extracts obtained from dichloromethane did not show significant activity in comparison with the hexane extracts. Hence the metabolites of Ulva reticulata and the bacterial secondary metabolites of the endophytes could be used in the treatment of bacterial infections.
