Background: To determine the concentration of group IIA phospholipase A2 (GIIAPLA2) in tears of patients with atopic blepharoconjunctivitis (ABC), and to compare it with the GIIAPLA2 conce ntration of tears in age-mat...Background: To determine the concentration of group IIA phospholipase A2 (GIIAPLA2) in tears of patients with atopic blepharoconjunctivitis (ABC), and to compare it with the GIIAPLA2 conce ntration of tears in age-matched healthy controls. Methods: The diagnosis of AB C was confirmed with a positive skin prick test and the presence of atopic derma titis in lids. Conjunctival brush cytology was taken, and the cells including eo sinophils, neutrophils, lymphocytes, squamous epithelial cells, columnar epithel ial cells, metaplastic changes and the goblet cells were calculated separately. The GIIAPLA2 concentration of tears was measured with a time-resolved fluoroimm unoassay in 29 patients with ABC (mean age 36.3±12.7 years) and 29 normal subje cts (mean age 37.0±12.0 years). Results: The GIIAPLA2 concentration of tears in patients with ABC was 43.8±33.0 μg/ml, and in normal subjects it was 67.1±23 .3 μg/ml. The difference was statistically significant (P=0.001 8). The concent ration of GIIAPLA2 of tears was lowest in the subgroup of patients with ABC and dry eye (25.8 ±23.6 μg/ml), whereas it was only slightly decreased in patients with ABC and normal tear secretion (56.6±33.3 μg/ml). The difference between these two subgroups was statistically significant (P=0.011). There was no statis tically significant correlation between the GIIAPLA2 concentration of tears and the quantity of different conjunctival cells gathered by the brush cytology. How ever, an almost significant correlation was found between the GIIAPLA2 concentra tion in tears and conjunctival eosinophils. Conclusions: The results indicate th at in patients with ABC the GIIAPLA2 content of tears was decreased, without any dependence on the quantity of different conjunctival cells.展开更多
Background: Sebaceous carcinoma (SC) is well known for its ability to masquerade clinically and histologically as a variety of periocular conditions resulting in a delayed diagnosis. We present a series of periocular ...Background: Sebaceous carcinoma (SC) is well known for its ability to masquerade clinically and histologically as a variety of periocular conditions resulting in a delayed diagnosis. We present a series of periocular SC cases and discuss the difficulties in histopathological diagnosis when this tumor presents with a Bowenoid pattern of intraepithelial spread. Methods: A retrospective case study of all patients with SC of the eyelid treated in our Hospital, from 1997 to 2004, was conducted. Results: Eight patients were identified (four females and four males). Seven cases involved the upper eyelid. Initial clinical diagnoses included blepharitis (three cases), blepharoconjunctivitis (one case), cicatrizing conjunctivitis (one case), and lid lesions (two cases). Histopathologically, 87.5%of cases were misdiagnosed as Bowen’s disease (BD) on the initial biopsy. Six of these cases showed no invasive disease on the initial biopsy and were eventually found to be invasive SC on subsequent excisions. In one case, the tumor was wholly in situ. Delay in diagnosis ranged from 0 to 56 months. Conclusions: SC should always be considered in the histological differential diagnosis of any eyelid lesion which resembles BD, particularly if the upper eyelid is involved or if multivacuolated cytoplasmic clear cell changes are seen.展开更多
文摘Background: To determine the concentration of group IIA phospholipase A2 (GIIAPLA2) in tears of patients with atopic blepharoconjunctivitis (ABC), and to compare it with the GIIAPLA2 conce ntration of tears in age-matched healthy controls. Methods: The diagnosis of AB C was confirmed with a positive skin prick test and the presence of atopic derma titis in lids. Conjunctival brush cytology was taken, and the cells including eo sinophils, neutrophils, lymphocytes, squamous epithelial cells, columnar epithel ial cells, metaplastic changes and the goblet cells were calculated separately. The GIIAPLA2 concentration of tears was measured with a time-resolved fluoroimm unoassay in 29 patients with ABC (mean age 36.3±12.7 years) and 29 normal subje cts (mean age 37.0±12.0 years). Results: The GIIAPLA2 concentration of tears in patients with ABC was 43.8±33.0 μg/ml, and in normal subjects it was 67.1±23 .3 μg/ml. The difference was statistically significant (P=0.001 8). The concent ration of GIIAPLA2 of tears was lowest in the subgroup of patients with ABC and dry eye (25.8 ±23.6 μg/ml), whereas it was only slightly decreased in patients with ABC and normal tear secretion (56.6±33.3 μg/ml). The difference between these two subgroups was statistically significant (P=0.011). There was no statis tically significant correlation between the GIIAPLA2 concentration of tears and the quantity of different conjunctival cells gathered by the brush cytology. How ever, an almost significant correlation was found between the GIIAPLA2 concentra tion in tears and conjunctival eosinophils. Conclusions: The results indicate th at in patients with ABC the GIIAPLA2 content of tears was decreased, without any dependence on the quantity of different conjunctival cells.
文摘Background: Sebaceous carcinoma (SC) is well known for its ability to masquerade clinically and histologically as a variety of periocular conditions resulting in a delayed diagnosis. We present a series of periocular SC cases and discuss the difficulties in histopathological diagnosis when this tumor presents with a Bowenoid pattern of intraepithelial spread. Methods: A retrospective case study of all patients with SC of the eyelid treated in our Hospital, from 1997 to 2004, was conducted. Results: Eight patients were identified (four females and four males). Seven cases involved the upper eyelid. Initial clinical diagnoses included blepharitis (three cases), blepharoconjunctivitis (one case), cicatrizing conjunctivitis (one case), and lid lesions (two cases). Histopathologically, 87.5%of cases were misdiagnosed as Bowen’s disease (BD) on the initial biopsy. Six of these cases showed no invasive disease on the initial biopsy and were eventually found to be invasive SC on subsequent excisions. In one case, the tumor was wholly in situ. Delay in diagnosis ranged from 0 to 56 months. Conclusions: SC should always be considered in the histological differential diagnosis of any eyelid lesion which resembles BD, particularly if the upper eyelid is involved or if multivacuolated cytoplasmic clear cell changes are seen.
