The development of biochips for the detection of nucleic acids, proteins or enzyme-substrate interactions has experienced a large increase since those tools were found to provide to researchers and diagnostic companie...The development of biochips for the detection of nucleic acids, proteins or enzyme-substrate interactions has experienced a large increase since those tools were found to provide to researchers and diagnostic companies a rapid sample’s screening. Nevertheless, one of the main handicap for the development in this area appeared to be a lack of generic immobilization procedure for those biologically active compounds; a lack linked in one hand to the obligatory effort to keep active the immobilized biological molecules and in the other hand to the diversity of detection/transduction systems used. The early immobilization procedure on silicon and glass support based on silane technology, still in use for protein and DNA immobilization [1-3] is now in competition with thiol-disulfide techniques [4-6] on gold surface, beads array [7, 8], micro-contact printing [9] and particle lithography [10]. Each of these techniques is used in conjunction with a detection system, most of the time intimately dependent of the immobilization procedure.展开更多
文摘The development of biochips for the detection of nucleic acids, proteins or enzyme-substrate interactions has experienced a large increase since those tools were found to provide to researchers and diagnostic companies a rapid sample’s screening. Nevertheless, one of the main handicap for the development in this area appeared to be a lack of generic immobilization procedure for those biologically active compounds; a lack linked in one hand to the obligatory effort to keep active the immobilized biological molecules and in the other hand to the diversity of detection/transduction systems used. The early immobilization procedure on silicon and glass support based on silane technology, still in use for protein and DNA immobilization [1-3] is now in competition with thiol-disulfide techniques [4-6] on gold surface, beads array [7, 8], micro-contact printing [9] and particle lithography [10]. Each of these techniques is used in conjunction with a detection system, most of the time intimately dependent of the immobilization procedure.
