分离、鉴定除草剂咪唑乙烟酸降解菌IM9603并优化其降解条件。通过形态学,生理生化反应,16S r DNA初步鉴定该菌株分类地位,利用Box-Behnken优化该菌株对咪唑乙烟酸的降解条件。结果显示,初步鉴定该菌株属于表短杆菌属,在条件(p H5.5,温度...分离、鉴定除草剂咪唑乙烟酸降解菌IM9603并优化其降解条件。通过形态学,生理生化反应,16S r DNA初步鉴定该菌株分类地位,利用Box-Behnken优化该菌株对咪唑乙烟酸的降解条件。结果显示,初步鉴定该菌株属于表短杆菌属,在条件(p H5.5,温度26.2℃,接菌量1 m L(OD600=1))下降解能力最强,菌株IM9603在咪唑乙烟酸初始浓度为50 mg/L的条件下在7d内降解率可达93.08%。分离出的咪唑乙烟酸降解菌属于Brevibacterium epidermidis菌株,该菌株对咪唑乙烟酸具有较高的降解能力,在环境修复方面存在潜在的应用前景。展开更多
Two chromium-resistant bacterial strains CrT-1 and CrT-13,which can tolerate K2 CrO4 up to 40 mg·mL-1 on nutrient agar,25 mg·mL-1 K2 CrO4 in nutrient broth,and up to 10 mg·mL-1 in acetate-minimal media,...Two chromium-resistant bacterial strains CrT-1 and CrT-13,which can tolerate K2 CrO4 up to 40 mg·mL-1 on nutrient agar,25 mg·mL-1 K2 CrO4 in nutrient broth,and up to 10 mg·mL-1 in acetate-minimal media,were used in this study.On the basis of 16S rRNA,strain CrT-1 was identified as Ochrobactrum intermedium and CrT-13 as Brevibacterium sp..Uptake of chromate was greater in living cells than in heat-killed cells.Ochrobactrum intermedium CrT-1 reduced 73％ and 41％ of Cr(Ⅵ)while Brevibacterium CrT-13 reduced 62％ and 48％ Cr(Ⅵ) at an initial chromate concentration of 750,and 1500 μg·mL-1,after 96 hours with an inoculum size of 9.6×107 cells·mL-1.Different heavy metals at low concentrations did not affect the reduction potential of
the strains significantly.Ochrobactrum intermedium CrT-1 reduced 84％ and 65％ while Brevibacterium CrT-13 reduced 60％ and 44％ of Cr(Ⅵ)at an initial Cr(Ⅵ)concentration of 250 and 500 μg·mL-1,espectively,in an industrial effluent sample.展开更多
文摘分离、鉴定除草剂咪唑乙烟酸降解菌IM9603并优化其降解条件。通过形态学,生理生化反应,16S r DNA初步鉴定该菌株分类地位,利用Box-Behnken优化该菌株对咪唑乙烟酸的降解条件。结果显示,初步鉴定该菌株属于表短杆菌属,在条件(p H5.5,温度26.2℃,接菌量1 m L(OD600=1))下降解能力最强,菌株IM9603在咪唑乙烟酸初始浓度为50 mg/L的条件下在7d内降解率可达93.08%。分离出的咪唑乙烟酸降解菌属于Brevibacterium epidermidis菌株,该菌株对咪唑乙烟酸具有较高的降解能力,在环境修复方面存在潜在的应用前景。
文摘Two chromium-resistant bacterial strains CrT-1 and CrT-13,which can tolerate K2 CrO4 up to 40 mg·mL-1 on nutrient agar,25 mg·mL-1 K2 CrO4 in nutrient broth,and up to 10 mg·mL-1 in acetate-minimal media,were used in this study.On the basis of 16S rRNA,strain CrT-1 was identified as Ochrobactrum intermedium and CrT-13 as Brevibacterium sp..Uptake of chromate was greater in living cells than in heat-killed cells.Ochrobactrum intermedium CrT-1 reduced 73％ and 41％ of Cr(Ⅵ)while Brevibacterium CrT-13 reduced 62％ and 48％ Cr(Ⅵ) at an initial chromate concentration of 750,and 1500 μg·mL-1,after 96 hours with an inoculum size of 9.6×107 cells·mL-1.Different heavy metals at low concentrations did not affect the reduction potential of
the strains significantly.Ochrobactrum intermedium CrT-1 reduced 84％ and 65％ while Brevibacterium CrT-13 reduced 60％ and 44％ of Cr(Ⅵ)at an initial Cr(Ⅵ)concentration of 250 and 500 μg·mL-1,espectively,in an industrial effluent sample.
