从污染场地土壤中筛选出一株能够以石油烃为唯一碳源和能源的降解菌株,并命名为ZR-1。经菌体形态观察,16S r DNA序列分析初步鉴定菌株为Alcaligenaceae.sp,同源性为97%。该菌株可以在0%~3%的盐度条件下生长,属于轻度嗜盐菌。在Na Cl质...从污染场地土壤中筛选出一株能够以石油烃为唯一碳源和能源的降解菌株,并命名为ZR-1。经菌体形态观察,16S r DNA序列分析初步鉴定菌株为Alcaligenaceae.sp,同源性为97%。该菌株可以在0%~3%的盐度条件下生长,属于轻度嗜盐菌。在Na Cl质量分数为3%,柴油浓度为400mg/L的条件下,96h降解率可达60.425%,为今后生物修复高盐石油烃污染土壤奠定了一定的基础。展开更多
Because of the special physico-chemical properties and toxicity of oil in ocean, it causes the acute and chronic toxicity in marine life, through food chain comes into human body, has a serious harm on human＇s health...Because of the special physico-chemical properties and toxicity of oil in ocean, it causes the acute and chronic toxicity in marine life, through food chain comes into human body, has a serious harm on human＇s health. This test analysis influence of oil pollution in ocean, provides theoretical guidance of governing.展开更多
A computational fluid dynamics(CFD)numerical simulation and field experiment were used to investigate optimal operating parameters of high-pressure jet grouting equipment and clarify the boundary law of the injection ...A computational fluid dynamics(CFD)numerical simulation and field experiment were used to investigate optimal operating parameters of high-pressure jet grouting equipment and clarify the boundary law of the injection area in the remediation process.The response surface optimization design results show that the optimal injection pressure is 30 MPa,rotation speed is 23 r/min,commission speed is 30 cm/min,and the optimal injection diameter is 147.3 cm.Based on the CFD numerical simulation,the ratio of the injection core,turbulent zone,and seepage zone is approximately 1∶4∶2.The distribution law of jet core,turbulence zone and seepage zone at different cross-sections under 30 MPa operating conditions is as follows:The jet core radius is approximately 100 mm,the turbulence zone is mainly distributed at 100 to 500 mm,the seepage zone is mainly distributed at 500 to 700 mm,the seepage zone could be completed within 2 h,and the proportion of the three boundary zones in the injection zone is similar to that of the numerical simulation.This study provides theoretical parameters and practical reference for the remediation of deep pollution via in-situ chemical oxidation in the Loess Plateau soil environment.展开更多
A bench-scale biopiling experiment was conducted to hydrocarbon bioremediation in a chronically contaminated soil compare the ability of different techniques to enhance petroleum After 195 days, 10%-32% removal of TP...A bench-scale biopiling experiment was conducted to hydrocarbon bioremediation in a chronically contaminated soil compare the ability of different techniques to enhance petroleum After 195 days, 10%-32% removal of TPHs （total petroleum hydrocarbons） occurred in unamended soil （control）. Biostimulation by inorganic nutrient addition enhanced TPH removal （49%） confirming that bioremediation was nutrient limited and the soil contained a well-adapted hydrocarbonoclastic microbial community. The addition of organic amendments including green waste at 25% and 50% （w/w） and a commercial product called DaramendTM had a further biostimulatory effect （50%-66%, 34%-59% and 69%-80% TPH removal respectively）. Bioaugmentation using two commercially available petroleum hydrocarbon degrading microbial cultures with nutrients enhanced TPH removal in the case of RemActivTM （60%-69%）, but had a marginal effect using Recycler 102 （49%-55%）. The effect of a non-ionic surfactant in green waste amended soil was variable （52%-72% TPH reduction）, but its potential to enhance biodegradation presumably by promoting contaminant bioavailability was demonstrated. High degradation of artificially added polycyclic aromatic hydrocarbons （PAHs） occurred after 106 days （75%-84%）, but significant differences between the control and treatments were unapparent, suggesting that spiked soils do not reflect the behavior of contaminants in genuinely polluted and weathered soil.展开更多
It has been studied restoration processes in oil products-polluted soils at high northern latitudes （the Murmansk region, Russia）. Mineral and organic fertilizers and a bacterial preparation （based on the local str...It has been studied restoration processes in oil products-polluted soils at high northern latitudes （the Murmansk region, Russia）. Mineral and organic fertilizers and a bacterial preparation （based on the local strains of hydrocarbon-oxidizing bacteria） were applied for restore polluted soils. Periods of removing OP （oil products） from soil were determined by the reduction of the pollutant concentration and by soil biological activities--the dynamics of bacteria number and CO2 emission from soil. The soil OP even at such a high concentration （as 10 L/m^2） had stimulated bacterial reproduction. In three summer month levels in the control variant without ameliorators of OP content decreased by 59% from the initial level, in the variant with mineral and organic fertilizers by 86%, in the variant with the bacterial preparation by 84%. Stimulating of indigenous microorganisms activity with additional nutrients was no less effective technique of OP-polluted soil bioremediation, than applying the bacterial preparation, which requires considerable financial investment. Moderately contaminated of OP soil is a source of additional carbon dioxide emission in the atmosphere. Pollution soil with OP caused for increasing of share of potentially pathogenic fungi in the fungal community.展开更多
In this paper, we conduct research on the progress of environmental remediation methodologies under the conditions of oil and heavy metal pollution. The main repair contaminated soil measures include chemical, physica...In this paper, we conduct research on the progress of environmental remediation methodologies under the conditions of oil and heavy metal pollution. The main repair contaminated soil measures include chemical, physical and chemical measures, biological repair measures and agricultural ecological measures, engineering measures, etc. Selection principle is to adjust measures to local conditions, at the same time, take the technical, economic and effects of factors such as accessibility. After comparing the mentioned techniques, we conclude that the bioremediation method holds the best effectiveness for us to make contribution to the environmental protection. In final part, we give the conclusion and sct up the prospect.展开更多
Considerable efforts have been undertaken to accelerate the breakdown of existing anthropogenic petroleum hydrocarbons （PHCs） by appropriate in situ remediation technologies. In situ phytoremediation, using higher p...Considerable efforts have been undertaken to accelerate the breakdown of existing anthropogenic petroleum hydrocarbons （PHCs） by appropriate in situ remediation technologies. In situ phytoremediation, using higher plants to remove, stabilize, degrade, and/or metabolize hazardous contaminants, has emerged as a promising green technology for cleaning up environments contaminated with PHCs. Weed plants are generally considered to have great potential for use in phytoremediation due to their extensive fibrous root systems and relatively robust characteristics, thus helping establish a strong rhizosphere through contaminated soils. In this review, some important mechanisms involved in phytoremedation of PHC contaminated soils, including phytoaccumulation, phytostabilization, phytodegradation, phytovolatilization, and rhizodegradation, were summarized and discussed. In recent years, a large number of laboratory approaches have been developed to further enhance the phytoremediation efficiency of PHC contaminated soils. The success of these laboratory studies has encouraged researchers to attempt phytoremediation of PHC contaminated soils in the field. However, many limitations still exist in order to successfully apply laboratory experiments to trials in the field.展开更多
文摘从污染场地土壤中筛选出一株能够以石油烃为唯一碳源和能源的降解菌株,并命名为ZR-1。经菌体形态观察,16S r DNA序列分析初步鉴定菌株为Alcaligenaceae.sp,同源性为97%。该菌株可以在0%~3%的盐度条件下生长,属于轻度嗜盐菌。在Na Cl质量分数为3%,柴油浓度为400mg/L的条件下,96h降解率可达60.425%,为今后生物修复高盐石油烃污染土壤奠定了一定的基础。
文摘Because of the special physico-chemical properties and toxicity of oil in ocean, it causes the acute and chronic toxicity in marine life, through food chain comes into human body, has a serious harm on human＇s health. This test analysis influence of oil pollution in ocean, provides theoretical guidance of governing.
基金The National Natural Science Foundation of China(No.41967043,52160003)the Natural Science Foundation of Gansu Province(No.20JR5RA461)+1 种基金the Key Project of China Railway Southwest Research Institute Co.,Ltd.(No.2018-KJ003-Z003-XB)the Industrial Support Program of the Higher Education of Gansu Province(No.2020C-40).
文摘A computational fluid dynamics(CFD)numerical simulation and field experiment were used to investigate optimal operating parameters of high-pressure jet grouting equipment and clarify the boundary law of the injection area in the remediation process.The response surface optimization design results show that the optimal injection pressure is 30 MPa,rotation speed is 23 r/min,commission speed is 30 cm/min,and the optimal injection diameter is 147.3 cm.Based on the CFD numerical simulation,the ratio of the injection core,turbulent zone,and seepage zone is approximately 1∶4∶2.The distribution law of jet core,turbulence zone and seepage zone at different cross-sections under 30 MPa operating conditions is as follows:The jet core radius is approximately 100 mm,the turbulence zone is mainly distributed at 100 to 500 mm,the seepage zone is mainly distributed at 500 to 700 mm,the seepage zone could be completed within 2 h,and the proportion of the three boundary zones in the injection zone is similar to that of the numerical simulation.This study provides theoretical parameters and practical reference for the remediation of deep pollution via in-situ chemical oxidation in the Loess Plateau soil environment.
文摘A bench-scale biopiling experiment was conducted to hydrocarbon bioremediation in a chronically contaminated soil compare the ability of different techniques to enhance petroleum After 195 days, 10%-32% removal of TPHs （total petroleum hydrocarbons） occurred in unamended soil （control）. Biostimulation by inorganic nutrient addition enhanced TPH removal （49%） confirming that bioremediation was nutrient limited and the soil contained a well-adapted hydrocarbonoclastic microbial community. The addition of organic amendments including green waste at 25% and 50% （w/w） and a commercial product called DaramendTM had a further biostimulatory effect （50%-66%, 34%-59% and 69%-80% TPH removal respectively）. Bioaugmentation using two commercially available petroleum hydrocarbon degrading microbial cultures with nutrients enhanced TPH removal in the case of RemActivTM （60%-69%）, but had a marginal effect using Recycler 102 （49%-55%）. The effect of a non-ionic surfactant in green waste amended soil was variable （52%-72% TPH reduction）, but its potential to enhance biodegradation presumably by promoting contaminant bioavailability was demonstrated. High degradation of artificially added polycyclic aromatic hydrocarbons （PAHs） occurred after 106 days （75%-84%）, but significant differences between the control and treatments were unapparent, suggesting that spiked soils do not reflect the behavior of contaminants in genuinely polluted and weathered soil.
文摘It has been studied restoration processes in oil products-polluted soils at high northern latitudes （the Murmansk region, Russia）. Mineral and organic fertilizers and a bacterial preparation （based on the local strains of hydrocarbon-oxidizing bacteria） were applied for restore polluted soils. Periods of removing OP （oil products） from soil were determined by the reduction of the pollutant concentration and by soil biological activities--the dynamics of bacteria number and CO2 emission from soil. The soil OP even at such a high concentration （as 10 L/m^2） had stimulated bacterial reproduction. In three summer month levels in the control variant without ameliorators of OP content decreased by 59% from the initial level, in the variant with mineral and organic fertilizers by 86%, in the variant with the bacterial preparation by 84%. Stimulating of indigenous microorganisms activity with additional nutrients was no less effective technique of OP-polluted soil bioremediation, than applying the bacterial preparation, which requires considerable financial investment. Moderately contaminated of OP soil is a source of additional carbon dioxide emission in the atmosphere. Pollution soil with OP caused for increasing of share of potentially pathogenic fungi in the fungal community.
文摘In this paper, we conduct research on the progress of environmental remediation methodologies under the conditions of oil and heavy metal pollution. The main repair contaminated soil measures include chemical, physical and chemical measures, biological repair measures and agricultural ecological measures, engineering measures, etc. Selection principle is to adjust measures to local conditions, at the same time, take the technical, economic and effects of factors such as accessibility. After comparing the mentioned techniques, we conclude that the bioremediation method holds the best effectiveness for us to make contribution to the environmental protection. In final part, we give the conclusion and sct up the prospect.
基金National Natural Science Foundation of China (4093073921037002)
文摘Considerable efforts have been undertaken to accelerate the breakdown of existing anthropogenic petroleum hydrocarbons （PHCs） by appropriate in situ remediation technologies. In situ phytoremediation, using higher plants to remove, stabilize, degrade, and/or metabolize hazardous contaminants, has emerged as a promising green technology for cleaning up environments contaminated with PHCs. Weed plants are generally considered to have great potential for use in phytoremediation due to their extensive fibrous root systems and relatively robust characteristics, thus helping establish a strong rhizosphere through contaminated soils. In this review, some important mechanisms involved in phytoremedation of PHC contaminated soils, including phytoaccumulation, phytostabilization, phytodegradation, phytovolatilization, and rhizodegradation, were summarized and discussed. In recent years, a large number of laboratory approaches have been developed to further enhance the phytoremediation efficiency of PHC contaminated soils. The success of these laboratory studies has encouraged researchers to attempt phytoremediation of PHC contaminated soils in the field. However, many limitations still exist in order to successfully apply laboratory experiments to trials in the field.
