为获得细胞膜受冲击破损的实际应力应变值。应用原子力显微镜(atomic force microscope,AFM)对Hela细胞进行破膜实验,得到细胞几何参数和加载过程的力-位移曲线。基于实验所得表面形貌与材料参数建立细胞破损有限元模型,并使用ABAQUS进...为获得细胞膜受冲击破损的实际应力应变值。应用原子力显微镜(atomic force microscope,AFM)对Hela细胞进行破膜实验,得到细胞几何参数和加载过程的力-位移曲线。基于实验所得表面形貌与材料参数建立细胞破损有限元模型,并使用ABAQUS进行分析。当针尖位移达到2.35μm时,实验所得破膜力为38.7 nN,模拟所得破膜力为39.3 nN,两者较为吻合,验证了模型的合理性和正确性。通过模拟获得细胞膜被穿破时,应力为24.3 kPa,塑性应变为0.23。本研究基于实验建立的细胞破损有限元模型,为模拟细胞的失效与破损过程提供了方法和参考。展开更多
This paper studied the breakthrough pressure for liquid water to penetrate the gas diffusion layer(GDL) of a proton exchange membrane fuel cell(PEMFC).An ex-situ testing was conducted on a transparent test cell to vis...This paper studied the breakthrough pressure for liquid water to penetrate the gas diffusion layer(GDL) of a proton exchange membrane fuel cell(PEMFC).An ex-situ testing was conducted on a transparent test cell to visualize the water droplet formation and detachment on the surface of different types of GDLs through a CCD camera.The breakthrough pressure,at which the liquid water penetrates the GDL and starts to form a droplet,was measured.The breakthrough pressure was found to be different for the GDLs with different porosities and thicknesses.The equilibrium pressure,which is defined as the minimum pressure required maintaining a constant flow through the GDL,was also recorded.The equilibrium pressure was found to be much lower than the breakthrough pressure for the same type of GDL.A pore network model was modified to further study the relationship between the breakthrough pressure and the GDL properties and thicknesses.The breakthrough pressure increases for the thick GDL with smaller micro-pore size.展开更多
基金the National Natural Science Foundation of China (No.50776006,No.50976011)
文摘This paper studied the breakthrough pressure for liquid water to penetrate the gas diffusion layer(GDL) of a proton exchange membrane fuel cell(PEMFC).An ex-situ testing was conducted on a transparent test cell to visualize the water droplet formation and detachment on the surface of different types of GDLs through a CCD camera.The breakthrough pressure,at which the liquid water penetrates the GDL and starts to form a droplet,was measured.The breakthrough pressure was found to be different for the GDLs with different porosities and thicknesses.The equilibrium pressure,which is defined as the minimum pressure required maintaining a constant flow through the GDL,was also recorded.The equilibrium pressure was found to be much lower than the breakthrough pressure for the same type of GDL.A pore network model was modified to further study the relationship between the breakthrough pressure and the GDL properties and thicknesses.The breakthrough pressure increases for the thick GDL with smaller micro-pore size.
