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水泥混凝土路面大修工程应用冲压破裂稳固技术的试验段实例
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作者 罗伟 《建筑技术开发》 2008年第8期69-71,共3页
国、省道路面大修工程应用蓝派冲压破裂稳固技术对旧水泥混凝土路面进行处治,通过蓝派冲击压路机施工试验路段的方案设计及其结果分析总结,介绍了水泥混凝土路面大修工程中应用冲压破裂稳固技术的施工工艺,提出合理可行的检测方法和质... 国、省道路面大修工程应用蓝派冲压破裂稳固技术对旧水泥混凝土路面进行处治,通过蓝派冲击压路机施工试验路段的方案设计及其结果分析总结,介绍了水泥混凝土路面大修工程中应用冲压破裂稳固技术的施工工艺,提出合理可行的检测方法和质量控制标准。 展开更多
关键词 水泥混凝土路面 大修工程 冲压破裂稳固技术
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雅砻水库工程静态膨胀剂破解孤石的施工技术
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作者 陈龙 娄旭峰 王海艳 《四川水利》 2016年第S2期64-67,共4页
静态破裂膨胀材料在不同的环境和气候中,适应性各不相同,特别是在高原寒冷地区,应用静态破裂技术极为少见.本文主要介绍在西藏雅砻水库工程料场开采施工中,揭露有大量孤石群,对取料产生了较大影响,在无炸药的情况下,采用了静态膨胀破碎... 静态破裂膨胀材料在不同的环境和气候中,适应性各不相同,特别是在高原寒冷地区,应用静态破裂技术极为少见.本文主要介绍在西藏雅砻水库工程料场开采施工中,揭露有大量孤石群,对取料产生了较大影响,在无炸药的情况下,采用了静态膨胀破碎剂破解孤石群,取得了良好的效果,获得了成功的经验. 展开更多
关键词 高海拔 静态 膨胀剂 破裂技术 孤石群 雅砻水库工程
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地面微地震监测技术研究及在牟页1井水力压裂中的应用 被引量:1
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作者 赵百顺 黄芮 +3 位作者 冯辉 汪超 刘红亮 陈华涛 《能源技术与管理》 2019年第1期166-168,共3页
通过微地震监测技术来判断裂缝系统对于后期优化压裂设计、校正裂缝模型及井位部署具有重要的意义。在中牟页岩气区块牟页1井水力压裂过程中,利用在井口周围布设的59个浅钻稀疏三分量数字检波器组成的观测系统采集了微地震信号,并且利... 通过微地震监测技术来判断裂缝系统对于后期优化压裂设计、校正裂缝模型及井位部署具有重要的意义。在中牟页岩气区块牟页1井水力压裂过程中,利用在井口周围布设的59个浅钻稀疏三分量数字检波器组成的观测系统采集了微地震信号,并且利用微破裂向量扫描数据处理技术成功地对水力压裂裂缝进行了成像。通过对压裂过程中破裂能量的时间及空间分布的分析,获得了水力裂缝延伸的动态过程。通过对微地震监测结果进行分析,解释了水力裂缝分布的状态,水力裂缝延伸长度及延伸的方位角,明确了水力压裂改造的储层体积。 展开更多
关键词 微地震监测 水力压裂 三分量检波器 破裂向量扫描技术
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超声微泡介导Itch基因沉默增强T细胞对肺腺癌细胞LA795免疫杀伤作用
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作者 毛宁 熊弢 《基础医学与临床》 CSCD 2018年第4期516-521,共6页
目的利用超声介导微泡破裂技术(UTMD)沉默T细胞Itch基因表达,观察转染T细胞对肺腺癌细胞LA795的体外免疫杀伤效率。方法磁珠分选纯化T细胞,构建靶向Itch基因的shRNA表达质粒,超声微泡介导转染48 h后,荧光显微镜和流式细胞仪评估细胞转... 目的利用超声介导微泡破裂技术(UTMD)沉默T细胞Itch基因表达,观察转染T细胞对肺腺癌细胞LA795的体外免疫杀伤效率。方法磁珠分选纯化T细胞,构建靶向Itch基因的shRNA表达质粒,超声微泡介导转染48 h后,荧光显微镜和流式细胞仪评估细胞转染效率,Western blot检测Itch蛋白表达。转染72 h后,酶联免疫吸附法(ELISA)检测细胞上清液中IL-2和IFN-γ分泌水平,观察对比单纯T细胞、阴性对照T细胞及转染T细胞与小鼠肺腺癌细胞LA795共培养时肿瘤杀伤率。结果利用UTMD技术介导shRNA转染效率达到52.3%±3.8%,Itch蛋白表达能够有效被抑制。转染72 h后,超声微泡介导沉默Itch基因显著增加T细胞因子IL-2和IFN-γ分泌水平(P<0.05);与空白组或阴性对照组T细胞相比,在不同的靶效比水平(10∶1、20∶1、40∶1),转染T细胞杀瘤活性均明显增高(P<0.05)。结论利用UTMD技术介导shRNA转染能有效沉默Itch基因表达,促进T细胞免疫活性,增强T细胞对小鼠肺腺癌细胞LA795的体外免疫杀伤效率。 展开更多
关键词 ITCH 超声介导微泡破裂技术 基因沉默 肺肿瘤 免疫治疗
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Influence of Perforation on Formation Fracturing Pressure 被引量:7
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作者 张广清 陈勉 +1 位作者 王学双 赵冲 《Petroleum Science》 SCIE CAS CSCD 2004年第3期56-61,共6页
Hydraulic fracturing treatments of oil wells are greatly affected by the perforation parameters selected. The three-dimensional finite element model together with the tensile criterion of rock materials is employed t... Hydraulic fracturing treatments of oil wells are greatly affected by the perforation parameters selected. The three-dimensional finite element model together with the tensile criterion of rock materials is employed to systematically investigate the influence of perforation parameters, such as perforation density, perforation orientation, perforation diameter, and perforation length as well as wellbore ellipticity, in vertical wells on the formation fracturing pressure. Based on a six-month simulation research in the University of Petroleum, China, several conclusions are drawn for the first time. Perforation density and perforation orientation angle are the most important parameters controlling the formation fracturing pressure. As the perforation density increases, the fracturing pressure decreases, not linearly but progressively. The fracturing pressure increases with the perforation orientation angle only when the angle is less than 45 degrees, and the relationship becomes very flat when the angle is 45 degrees. However, with regards to the perforation diameter and perforation length, their influences are much slighter. The wellbore ellipticity has a significant effect on the formation fracturing pressure. It is obvious that fracturing pressure increases linearly with the ellipticity of the wellbore. 展开更多
关键词 Fracturing pressure three-dimensional finite element perforation parameter
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超声微泡介导沉默T淋巴细胞Itch基因对胃癌MFC细胞免疫杀伤作用的实验研究 被引量:1
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作者 刘丹 张煜 +2 位作者 彭莉晴 周静 邹庆伟 《中国现代医学杂志》 CAS 2018年第9期22-27,共6页
目的利用超声介导微泡破裂技术(UTMD)沉默T淋巴细胞Itch基因表达,观察转染T细胞对胃癌MFC细胞的免疫杀伤效率。方法免疫磁珠分离T淋巴细胞,构建靶向Itch基因的sh RNA表达质粒,超声微泡介导转染48 h后,流式细胞仪分析T细胞转染成功率,Wes... 目的利用超声介导微泡破裂技术(UTMD)沉默T淋巴细胞Itch基因表达,观察转染T细胞对胃癌MFC细胞的免疫杀伤效率。方法免疫磁珠分离T淋巴细胞,构建靶向Itch基因的sh RNA表达质粒,超声微泡介导转染48 h后,流式细胞仪分析T细胞转染成功率,Western blot测定T细胞Itch蛋白表达。转染24 h后,流式细胞仪检测T淋巴细胞活化早期标志CD69表达。转染72 h后,观察对比单纯T淋巴细胞、阴性对照T淋巴细胞及转染T淋巴细胞与小鼠胃癌MFC细胞共培养时肿瘤杀伤率。结果利用UTMD技术介导sh RNA转染效率达到51.9%,Itch蛋白表达能被有效抑制。转染24 h后,转染组T淋巴细胞早期活化标志CD69表达较其他组更高。转染72 h后,与阴性对照组和空白组相比,在不同的效靶比水平(10∶1、20∶1、40∶1),转染T淋巴细胞杀瘤活性均增强。结论利用UTMD技术介导sh RNA转染能有效沉默Itch基因表达,促进T淋巴细胞免疫活性,增强T淋巴细胞对胃癌MFC细胞的免疫杀伤效率。 展开更多
关键词 ITCH 超声介导微泡破裂技术 基因沉默 胃癌 免疫治疗
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Field investigation into directional hydraulic fracturing for hard roof in Tashan Coal Mine 被引量:14
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作者 Bina-Xiana HUANG Bin YU +3 位作者 Feng FENG Zhao LI You-Zhuang WANG Jin-Rong LIU 《Journal of Coal Science & Engineering(China)》 2013年第2期153-159,共7页
t Research and development of safe and effective control technology of hard roof is an inevitable trend at present. Directional hydraulic fracturing technology is expected to become a safe and effective way to control... t Research and development of safe and effective control technology of hard roof is an inevitable trend at present. Directional hydraulic fracturing technology is expected to become a safe and effective way to control and manage hard roof. In order to make hard roof fracture in a directional way, a hydraulic fracture field test has been conducted in the third panel district of Tashan Coal Mine in Datong. First, two hydraulic fracturing drilling holes and four observing drilling holes were arranged in the roof, followed by a wedge-shaped ring slot in each hydraulic fracturing drilling hole. The hydraulic fracturing holes were then sealed and, hydraulic fracturing was conducted. The results show that the hard roof is fractured directionally by the hydraulic fracturing function of the two fracturing drilling holes; the sudden drop, or the overall downward trend of hydraulic pressure from hydraulic monitoring is the proof that the rock in the hard roof has been fractured. The required hydraulic pressure to fracture the hard roof in Tashan coal mine, consisting of carboniferous sandstone layer, is 50.09 MPa, and the fracturing radius of a single drilling hole is not less than 10.5 m. The wedge-shaped ring slot made in the bottom of the hydraulic fracturing drilling hole plays a guiding role for crack propagation. After the hydraulic fracturing drill hole is cracked, the propagation of the resulting hydraulic crack, affected mainly by the regional stress field, will turn to other directions. 展开更多
关键词 hard roof hydraulic fracturing wedge-shaped ring slot crack propagation
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Effectiveness analysis of methane-drainage by deep-hole controlled pre-splitting blasting for preventing coal and gas outburst 被引量:5
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作者 CAO Shu-gang LI Yong +2 位作者 LIU Yan-bao ZHANG Li-qiang XU A-meng 《Journal of Coal Science & Engineering(China)》 2009年第2期166-170,共5页
In the study of the application effectiveness of deep-hole controlled pre-splittingblasting technology,it was found through laboratory micro test and field study on a mine insouth China that under the technology,coal ... In the study of the application effectiveness of deep-hole controlled pre-splittingblasting technology,it was found through laboratory micro test and field study on a mine insouth China that under the technology,coal masses produce many irreversible cracks.Afterblasting,the nearer the distance from blasting hole,the larger the BET surface areaand volume ratio of the infiltration pore are;they increased by 11.47%and 5.73%,respectively.The coefficient of air permeability is increased 4 times.After 3 months,the gasdrainage rate was increased by 66%.In the first 15 days,the cumulative pumped gas was1.93 times of blasting before.The average absolute gas emission decreased by 63.46%.Experimental results show that deep-hole controlled pre-splitting blasting not only preventscoal and gas outburst,but also gives good economic results. 展开更多
关键词 coal and gas outburst methane-drainage pore structure deep-hole controlled pre-splitting blasting
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Mechanism on simulation and experiment of pre-crack seam formation in stope roof 被引量:1
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作者 胡建华 雷涛 +2 位作者 周科平 刘浪 劳德正 《Journal of Central South University》 SCIE EI CAS 2014年第4期1526-1533,共8页
The pre-crack blast technology has been used to control the induction caving area in the roof. The key is to form the pre-crack seam and predict the effect of the seam. The H-J-C blast model was built in the roof. Bas... The pre-crack blast technology has been used to control the induction caving area in the roof. The key is to form the pre-crack seam and predict the effect of the seam. The H-J-C blast model was built in the roof. Based on the theories of dynamic strength and failure criterion of dynamic rock, the rock dynamic damage and the evolution of pre-crack seam were simulated by the tensile damage and shear failure of the model. According to the actual situation of No. 92 ore body test stope at Tongkeng Mine, the formation process of the pre-crack blast seam was simulated by Ansys/Ls-dyna software, the pre-crack seam was inspected by a system of digital panoramic borehole camera. The pre-crack seam was inspected by the system of digital panoramic borehole in the roof. The results of the numerical simulation and inspection show that in the line of centers of pre-hole, the minimum of the tensile stress reaches 20 MPa, which is much larger than 13.7 MPa of the dynamic tensile strength of rock. The minimum particle vibration velocity reaches 50 cm/s, which is greater than 30-40 cm/s of the allowable vibration velocity. It is demonstrated that the rock is destroyed near the center line and the pre-crack is successfully formed by the large diameters and large distances pre-crack holes in the roof. 展开更多
关键词 induction caving dynamic damage model pre-crack seam numerical simulation digital borehole camera
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超声微泡介导CTLA-4基因靶向抑制对T淋巴细胞免疫活性及肿瘤免疫杀伤率影响的实验研究
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作者 韩志芬 饶程义 +1 位作者 康佳 陈玉 《中华肿瘤防治杂志》 CAS 北大核心 2023年第1期14-19,共6页
目的 利用超声介导微泡破裂技术(UTMD)介导T细胞转染,下调细胞毒性T细胞抗原-4(CTLA-4)基因表达,观察转染T细胞免疫活性变化,检测转染T细胞对MGC-803胃癌细胞的体外免疫杀伤率。方法 分离纯化C57小鼠脾脏T淋巴细胞,构建靶向CTLA-4基因... 目的 利用超声介导微泡破裂技术(UTMD)介导T细胞转染,下调细胞毒性T细胞抗原-4(CTLA-4)基因表达,观察转染T细胞免疫活性变化,检测转染T细胞对MGC-803胃癌细胞的体外免疫杀伤率。方法 分离纯化C57小鼠脾脏T淋巴细胞,构建靶向CTLA-4基因的短发夹RNA表达质粒,超声微泡介导CTLA-4质粒转染,设置转染组、阴性对照组和空白组,流式细胞仪分析转染率,实时荧光定量聚合酶链反应(qRT-PCR)检测CTLA-4基因表达,蛋白质印迹法检测各分组CTLA-4蛋白表达,流式细胞仪检测各分组T淋巴细胞活性标志CD25表达情况。按照30∶1靶效比将转染T淋巴细胞与MGC-803胃癌细胞共培养72 h,观察各分组体外肿瘤细胞杀伤率。结果 超声微泡介导shRNA转染率达71.6%,转染组CTLA-4基因和蛋白表达降低。转染组、阴性对照组和空白组CTLA-4 mRNA的表达分别为0.994±0.091、3.571±0.342和3.924±0.422,差异有统计学意义,F=86.374,P<0.001。转染组、阴性对照组和空白组CTLA-4蛋白相对表达量分别为0.153±0.031、0.517±0.102和0.499±0.094,差异有统计学意义,F=20.394,P=0.002。转染48 h后,转染组T细胞活化标志CD25表达升高,转染组、阴性对照组和空白组CD25表达分别为(29.13±3.73)%、(9.01±1.24)%和(9.33±1.07)%,差异有统计学意义,F=72.034,P<0.001。转染72 h后,转染T淋巴细胞肿瘤杀伤率增强,MGC-803胃癌细胞杀伤率达到(49.6±5.73)%,而阴性对照组和空白组MGC-803胃癌细胞杀伤率分别为(23.2±1.91)%和(21.6±1.57)%,差异有统计学意义,F=57.138,P<0.001。结论 通过UTMD介导转染能有效沉默CTLA-4基因和蛋白表达,上调T细胞免疫活性,增强T细胞对MGC-803胃癌细胞体外免疫杀伤作用。 展开更多
关键词 细胞毒性T细胞抗原-4 超声介导微泡破裂技术 基因沉默 T淋巴细胞 肿瘤免疫
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沉默T细胞Cbl-b基因对4T1细胞免疫杀伤作用影响
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作者 陈博 张萍 +1 位作者 谢宇平 段萍 《中华肿瘤防治杂志》 CAS 北大核心 2018年第4期226-231,共6页
目的乳腺癌发生发展与机体免疫水平有密切关系,而Cbl-b基因可作为抗肿瘤治疗的免疫靶点。利用超声介导微泡破裂技术(ultrasound-targeted microbubble destruction,UTMD)沉默T细胞Cbl-b基因表达,观察转染T细胞对4T1乳腺癌细胞的体外免... 目的乳腺癌发生发展与机体免疫水平有密切关系,而Cbl-b基因可作为抗肿瘤治疗的免疫靶点。利用超声介导微泡破裂技术(ultrasound-targeted microbubble destruction,UTMD)沉默T细胞Cbl-b基因表达,观察转染T细胞对4T1乳腺癌细胞的体外免疫杀伤效率。方法磁珠分选纯化T细胞,构建靶向Cbl-b基因的短发夹RNA(short-hairpin RNA,shRNA)表达质粒,超声微泡介导转染48h后,荧光显微镜和流式细胞仪评估细胞转染效率,蛋白质印迹法检测Cbl-b蛋白表达情况。转染72h后,利用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)比较各组细胞上清液中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)分泌水平。转染72h后,观察对比单纯T细胞、阴性对照T细胞及转染T细胞与小鼠4T1乳腺癌细胞共培养时肿瘤杀伤效率。结果原代培养T细胞纯度为93.7%。利用UTMD技术介导shRNA转染效率达到61.3%。qPCR和蛋白质印迹法检测结果显示,Cbl-b基因和蛋白水平表达均能被有效抑制。与空白组(1.007±0.022)相比,实验组Cbl-b mRNA表达量(0.333±0.046)明显降低,P<0.001;实验组、对照组和空白组Cbl-b蛋白相对表达量分别为0.301±0.080、0.773±0.101和0.719±0.090,检验统计量组间总变异,F=38.751,P<0.001;实验组Cbl-b蛋白表达显著降低。与空白组[(74±6)pg/mL]相比,实验组T细胞因子TNF-α分泌[(157±26)pg/mL]水平明显升高,P=0.001。转染72h后,与空白组及阴性对照组T细胞相比,在15∶1(P=0.046)、30∶1(P=0.028)和60∶1(P=0.003)的效靶比水平,转染T细胞杀瘤活性均明显增高。结论利用UTMD技术介导shRNA转染能有效沉默Cbl-b基因表达,促进T细胞免疫活性,增强T细胞对小鼠4T1乳腺癌细胞的体外免疫杀伤效率。 展开更多
关键词 CBL-B 超声介导微泡破裂技术 基因沉默 乳腺癌 免疫治疗
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Percutaneous elevation of depressed lunate fossa: A surgical technique 被引量:1
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作者 Atin Jaiswal Yashwant Singh Tanwar 《Chinese Journal of Traumatology》 CAS CSCD 2018年第5期304-307,共4页
Die-punch fractures or impaction fractures of distal radius articular surface are difficult to treat and hard to achieve satisfactory reduction, We present a unique, percutaneous and minimally invasive technique to el... Die-punch fractures or impaction fractures of distal radius articular surface are difficult to treat and hard to achieve satisfactory reduction, We present a unique, percutaneous and minimally invasive technique to elevate the depressed lunate fossa and maintain the reduction of the elevated fragment with no need of grafting in such fractures. This technique is simple, reproducible and can be executed with simple instrumentations. We think it deserves a variety of implications in the treatment of distal radius fractures. 展开更多
关键词 New surgical technique Depressed lunate fossa Percutaneous elevation
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Application of computer-assisted surgery techniques in the management of zygomatic complex fractures 被引量:2
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作者 Xiao Zhang Chao-Ying Han +6 位作者 Min-Jia Dai Jin-Long Chen Xiao-Hui Zheng Jie Long Wei Tang Wei-Dong Tian Lei Liu 《Chinese Journal of Traumatology》 CAS CSCD 2018年第5期281-286,共6页
Patients suffering from zygomatic complex fractures always present facial deformity and dyslunctions, and thereafter develop psychological and physiological problems. It is really hard to get an ideal prog- nosis for ... Patients suffering from zygomatic complex fractures always present facial deformity and dyslunctions, and thereafter develop psychological and physiological problems. It is really hard to get an ideal prog- nosis for the zygomatic complex fractures because of the complicated anatomical structures. Computer- assisted surgery techniques, as the new emerging auxiliary methods, can optimize the surgical protocol, predict operation outcomes, and improve the accuracy and quality of the operation. Meanwhile the postoperative complications can be reduced effectively. This review aims to provide a comprehensive overview of the application of computer-assisted surgery techniques in the management of zygomatic complex fractures. 展开更多
关键词 Zygomatic complex fractures Computer-assisted surgical techniques ACCURACY REDUCTION
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