通过体外培养人乳牙牙髓干细胞,研究炎症微环境对人乳牙牙髓干细胞促破骨相关因子表达的影响.采用体外酶消化结合组织块法培养人乳牙牙髓干细胞.将细胞分为对照组和实验组,对照组为正常培养液培养的人乳牙牙髓干细胞,实验组为含TNF-α...通过体外培养人乳牙牙髓干细胞,研究炎症微环境对人乳牙牙髓干细胞促破骨相关因子表达的影响.采用体外酶消化结合组织块法培养人乳牙牙髓干细胞.将细胞分为对照组和实验组,对照组为正常培养液培养的人乳牙牙髓干细胞,实验组为含TNF-α终浓度分别为10 ng/ML培养液培养的人乳牙牙髓干细胞;real time PCR检测破骨相关基因CTSK和TRAP的表达情况.酶消化加组织块法培养出来的乳牙牙髓干细胞大多呈克隆集落生长,成骨、成脂诱导结果可见钙化结节与脂滴.Real time PCR检测结果表明,与对照组相比,TNF-α刺激下人乳牙牙髓干细胞的破骨相关基因CTSK和TRAP的mRNA表达量明显上升,差异有统计学意义.炎症微环境可以导致人乳牙牙髓干细胞促破骨细胞形成能力增强,可能导致乳牙早失.展开更多
目的通过逆转录聚合酶链反应(RT-PCR)方法探讨姜黄素对脂多糖(LPS)刺激成骨细胞后骨保护因子(OPG)和破骨细胞核因子κB受体活化因子配基(RANKL)m RNA表达的影响。方法 1μg/m L LPS作用成骨细胞6、12、18、24、48 h,并用10μmol/L姜黄...目的通过逆转录聚合酶链反应(RT-PCR)方法探讨姜黄素对脂多糖(LPS)刺激成骨细胞后骨保护因子(OPG)和破骨细胞核因子κB受体活化因子配基(RANKL)m RNA表达的影响。方法 1μg/m L LPS作用成骨细胞6、12、18、24、48 h,并用10μmol/L姜黄素预处理成骨细胞60 min后加入1μg/m L LPS继续作用6、12、18、24、48 h,RT-PCR检测成骨细胞OPG和RANKLm RNA的表达。结果当以1μg/m L LPS作用于成骨细胞,随着作用时间的增加成骨细胞表达RANKL m RNA的能力逐渐增强,到24 h达到最高峰,到48 h基本恢复至初始水平;用10μmol/L姜黄素预处理成骨细胞,然后再用1μg/m L LPS处理成骨细胞48h,发现成骨细胞表达RANKL m RNA的能力明显减弱,随着作用时间的增加成骨细胞表达RANKL m RNA的能力变化不明显。当以1μg/m L LPS作用于成骨细胞,成骨细胞表达OPG m RNA的能力无明显变化;用10μmol/L姜黄素预处理成骨细胞,然后再用1μg/m L LPS处理成骨细胞48 h,发现成骨细胞表达OPG m RNA的能力也无明显变化。结论姜黄素可以减少LPS刺激后成骨细胞RANKL m RNA的表达,对OPG m RNA表达无影响,进而使得RANKL/OPG的比值减小,抑制骨吸收。展开更多
Purpose： Tibia plateau fracture （TPF） treatment aims at achieving a stable, aligned, mobile, painless knee and preventing post-traumatic osteoarthritis. To achieve this goal, surgeons consider criteria such as pati...Purpose： Tibia plateau fracture （TPF） treatment aims at achieving a stable, aligned, mobile, painless knee and preventing post-traumatic osteoarthritis. To achieve this goal, surgeons consider criteria such as patients＇ characteristics, severity, risk of complications, fracture displacementJdepression, degree of soft tissue injury. However, body mass index （BMI） is not considered as a risk factor in literature. Our study was conducted to find out any possible correlation between BMI and functional scores or radiological score separately. Methods： Retrospective analysis of case series between 2011 and 2014 was done on the database of a tertiary hospital in Istanbul. There were 67 TPF patients （54 males, 13 females） in the study. Relationship between BM1 and functional knee scores or radiological score was compared statistically. Closed fractures with both high-energy and low-energy injury were included in the study. Patients with open fracture, multi-trauma presence, meniscus and/or ligamentous injury, increased co-morbidity, inadequate records （25 cases in all） were excluded. Surgery type, Schatzker classification, injury side, trauma energy, and gender were considered as possible risk factors. Binary regression analysis was done for possible factors affecting functional knee scores and radiologic score. Results： Model summary calculations were done as Nagelkerke R2 test for Knee Society score, Lysholm knee score, and Ahlback and Rydberg radiologic scores, which were 0.648, 0.831, and 0.327 respec- tively. Homer-Lemeshow test values were 0.976, 0.998, and 0.362, respectively. There is negative correlation between BMI and both knee function scores. There is no correlation between BMI and radiologic score. Conclusion： An increase in BMI has a negative effect on functional knee scores after surgical treatment of TPFs. Therefore, BMI should be considered as a risk factor for surgical treatment of TPFs.展开更多
文摘通过体外培养人乳牙牙髓干细胞,研究炎症微环境对人乳牙牙髓干细胞促破骨相关因子表达的影响.采用体外酶消化结合组织块法培养人乳牙牙髓干细胞.将细胞分为对照组和实验组,对照组为正常培养液培养的人乳牙牙髓干细胞,实验组为含TNF-α终浓度分别为10 ng/ML培养液培养的人乳牙牙髓干细胞;real time PCR检测破骨相关基因CTSK和TRAP的表达情况.酶消化加组织块法培养出来的乳牙牙髓干细胞大多呈克隆集落生长,成骨、成脂诱导结果可见钙化结节与脂滴.Real time PCR检测结果表明,与对照组相比,TNF-α刺激下人乳牙牙髓干细胞的破骨相关基因CTSK和TRAP的mRNA表达量明显上升,差异有统计学意义.炎症微环境可以导致人乳牙牙髓干细胞促破骨细胞形成能力增强,可能导致乳牙早失.
文摘目的通过逆转录聚合酶链反应(RT-PCR)方法探讨姜黄素对脂多糖(LPS)刺激成骨细胞后骨保护因子(OPG)和破骨细胞核因子κB受体活化因子配基(RANKL)m RNA表达的影响。方法 1μg/m L LPS作用成骨细胞6、12、18、24、48 h,并用10μmol/L姜黄素预处理成骨细胞60 min后加入1μg/m L LPS继续作用6、12、18、24、48 h,RT-PCR检测成骨细胞OPG和RANKLm RNA的表达。结果当以1μg/m L LPS作用于成骨细胞,随着作用时间的增加成骨细胞表达RANKL m RNA的能力逐渐增强,到24 h达到最高峰,到48 h基本恢复至初始水平;用10μmol/L姜黄素预处理成骨细胞,然后再用1μg/m L LPS处理成骨细胞48h,发现成骨细胞表达RANKL m RNA的能力明显减弱,随着作用时间的增加成骨细胞表达RANKL m RNA的能力变化不明显。当以1μg/m L LPS作用于成骨细胞,成骨细胞表达OPG m RNA的能力无明显变化;用10μmol/L姜黄素预处理成骨细胞,然后再用1μg/m L LPS处理成骨细胞48 h,发现成骨细胞表达OPG m RNA的能力也无明显变化。结论姜黄素可以减少LPS刺激后成骨细胞RANKL m RNA的表达,对OPG m RNA表达无影响,进而使得RANKL/OPG的比值减小,抑制骨吸收。
文摘Purpose： Tibia plateau fracture （TPF） treatment aims at achieving a stable, aligned, mobile, painless knee and preventing post-traumatic osteoarthritis. To achieve this goal, surgeons consider criteria such as patients＇ characteristics, severity, risk of complications, fracture displacementJdepression, degree of soft tissue injury. However, body mass index （BMI） is not considered as a risk factor in literature. Our study was conducted to find out any possible correlation between BMI and functional scores or radiological score separately. Methods： Retrospective analysis of case series between 2011 and 2014 was done on the database of a tertiary hospital in Istanbul. There were 67 TPF patients （54 males, 13 females） in the study. Relationship between BM1 and functional knee scores or radiological score was compared statistically. Closed fractures with both high-energy and low-energy injury were included in the study. Patients with open fracture, multi-trauma presence, meniscus and/or ligamentous injury, increased co-morbidity, inadequate records （25 cases in all） were excluded. Surgery type, Schatzker classification, injury side, trauma energy, and gender were considered as possible risk factors. Binary regression analysis was done for possible factors affecting functional knee scores and radiologic score. Results： Model summary calculations were done as Nagelkerke R2 test for Knee Society score, Lysholm knee score, and Ahlback and Rydberg radiologic scores, which were 0.648, 0.831, and 0.327 respec- tively. Homer-Lemeshow test values were 0.976, 0.998, and 0.362, respectively. There is negative correlation between BMI and both knee function scores. There is no correlation between BMI and radiologic score. Conclusion： An increase in BMI has a negative effect on functional knee scores after surgical treatment of TPFs. Therefore, BMI should be considered as a risk factor for surgical treatment of TPFs.
