A novel bonded stationary phase, octadecanamido imine bonded stationary phase(ODAI), for reversed phase HPLC was prepared by bonding stearyl chloride to YWG 80 silica gel through 3 (2 aminoethylamino)propyltrimethoxys...A novel bonded stationary phase, octadecanamido imine bonded stationary phase(ODAI), for reversed phase HPLC was prepared by bonding stearyl chloride to YWG 80 silica gel through 3 (2 aminoethylamino)propyltrimethoxysilane. Hydrophobicity, selectivity and silanophilic activity of ODAI phase were evaluated by using aromatic compounds as analytes and methanol water as binary mobile phase. The organic components including acidic, basic, neutral aromatic analytes could be separated satisfactorily with an excellent selectivity and chromatographic peak shape. The asymmetry factors of basic aniline, o toluidine and N,N dimethylaniline were found to be 1 14, 1 06 and 1 01, respectively, using methanol water(volume ratio is 55∶45) as mobile phase. Aniline is eluted before phenol due to the internal masking interaction to suppress ion exchange activity of residual silanols.展开更多
文摘A novel bonded stationary phase, octadecanamido imine bonded stationary phase(ODAI), for reversed phase HPLC was prepared by bonding stearyl chloride to YWG 80 silica gel through 3 (2 aminoethylamino)propyltrimethoxysilane. Hydrophobicity, selectivity and silanophilic activity of ODAI phase were evaluated by using aromatic compounds as analytes and methanol water as binary mobile phase. The organic components including acidic, basic, neutral aromatic analytes could be separated satisfactorily with an excellent selectivity and chromatographic peak shape. The asymmetry factors of basic aniline, o toluidine and N,N dimethylaniline were found to be 1 14, 1 06 and 1 01, respectively, using methanol water(volume ratio is 55∶45) as mobile phase. Aniline is eluted before phenol due to the internal masking interaction to suppress ion exchange activity of residual silanols.
