Selenium （Se） is an important element from the environmental and biological point of view. In the world, deficiency of Se in a diet occurs more often and nutritional supplements are recommended.The ability of severa...Selenium （Se） is an important element from the environmental and biological point of view. In the world, deficiency of Se in a diet occurs more often and nutritional supplements are recommended.The ability of several plants to accumulate and transform inorganic Se forms into its bioactive organic compounds has important implications for human nutrition and health. This work gives a brief overview of the study carried out to characterize Se species produced by different enriched edible plants which were grown in the presence of different Se species.展开更多
In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nu...In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nutritional science. Se-yeast （Selenium-enriched yeast） is a common form of selenium used to supplement dietary intake of this important trace mineral. In the present study, we tried to prepare an organic selenocystine using locally isolated bread yeast （Saccharomyces cerevisiae）. A novel locally prepared date extract media enriched by addition of 0.2% KH2PO4 （potassium phosphate）, 0.6% ammonium sulfate was adopted as alternative culture media. Differences concentrations of selenium salt （30, 60, 120 and 240 lag/mL） were added to the yeast culture media. While the best concentration of selenium added was 30Bg/mL, it achieved optimal conditions for the growth of yeast and the production of red yeast growth identical to the standard. The products （organic selenocystine） were analyzed by HPLC （High Performance Liquid Chromatography） and AAS （Atomic Absorption Spectrophotometer） comparing with authentic standard obtained from Sigma. Results confirmed the formation of similar selenocystine products.展开更多
Ethaselen, an organoselenium compound designed and synthesized in the School of Pharmaceutical Sciences, Peking University, has been entitled to independent intellectual property rights both at home and abroad. As one...Ethaselen, an organoselenium compound designed and synthesized in the School of Pharmaceutical Sciences, Peking University, has been entitled to independent intellectual property rights both at home and abroad. As one of the novel antitumor drugs, ethaselen has been extensively studied in Phase I clinical trial, and its biological target is thioredoxin reductase. In this review, we focus on the ethaselen＇s efficacy and pharmacological actions, including antitumor effects both in vitro and in vivo, and immunologic functions. These research findings not only provide the theoretical basis for the anticancer study of ethaselen, but also guide the clinical trial of ethaselen.展开更多
A novel organoselenium compound,WB（1,2-[bis（1,2-benzisoselenazolone-3（2H）-ketone）]pentane） has indicated anti-tumor activity.Its pharmacokinetic data has never been determined.By using the H22 tumor bearing mous...A novel organoselenium compound,WB（1,2-[bis（1,2-benzisoselenazolone-3（2H）-ketone）]pentane） has indicated anti-tumor activity.Its pharmacokinetic data has never been determined.By using the H22 tumor bearing mouse model,the tissue distribution of WB after single and four consecutive doses（both were 120 mg/kg/d） was explored.The selenium content of the tissues was used as an indicator of WB absorption,distribution and metabolism.The selenium in the heart,liver, spleen,kidneys,lungs,stomach,pancreas,brain,colon,intestine,testes,plasma,and tumor were determined by generation atomic fluorescence spectrometry（AFS）.With single or multiple oral administration of WB,the selenium content significantly increased in the liver,stomach,colon,and intestine.The selenium content in the spleen,lungs,pancreas,testes,plasma and tumor also increased compared with the controls;but no significant changes were found in the brain and kidney.WB and its metabolites distributed predominantly in the colon,liver,stomach and intestine,which resulted in a significant increase in the selenium content in both groups.There was no observed significant accumulation of WB in the vital organs.展开更多
文摘Selenium （Se） is an important element from the environmental and biological point of view. In the world, deficiency of Se in a diet occurs more often and nutritional supplements are recommended.The ability of several plants to accumulate and transform inorganic Se forms into its bioactive organic compounds has important implications for human nutrition and health. This work gives a brief overview of the study carried out to characterize Se species produced by different enriched edible plants which were grown in the presence of different Se species.
文摘In contrast to the toxic inorganic forms of selenium to life at very low concentrations, the organic-selenium compounds are of considerable interest and several of them play essential roles in cell biochemistry and nutritional science. Se-yeast （Selenium-enriched yeast） is a common form of selenium used to supplement dietary intake of this important trace mineral. In the present study, we tried to prepare an organic selenocystine using locally isolated bread yeast （Saccharomyces cerevisiae）. A novel locally prepared date extract media enriched by addition of 0.2% KH2PO4 （potassium phosphate）, 0.6% ammonium sulfate was adopted as alternative culture media. Differences concentrations of selenium salt （30, 60, 120 and 240 lag/mL） were added to the yeast culture media. While the best concentration of selenium added was 30Bg/mL, it achieved optimal conditions for the growth of yeast and the production of red yeast growth identical to the standard. The products （organic selenocystine） were analyzed by HPLC （High Performance Liquid Chromatography） and AAS （Atomic Absorption Spectrophotometer） comparing with authentic standard obtained from Sigma. Results confirmed the formation of similar selenocystine products.
基金National Natural Science Foundation of China(Grant No.30472036)
文摘Ethaselen, an organoselenium compound designed and synthesized in the School of Pharmaceutical Sciences, Peking University, has been entitled to independent intellectual property rights both at home and abroad. As one of the novel antitumor drugs, ethaselen has been extensively studied in Phase I clinical trial, and its biological target is thioredoxin reductase. In this review, we focus on the ethaselen＇s efficacy and pharmacological actions, including antitumor effects both in vitro and in vivo, and immunologic functions. These research findings not only provide the theoretical basis for the anticancer study of ethaselen, but also guide the clinical trial of ethaselen.
基金National Major Projects on Drug Research and Technology(Grant No.2009ZX09103-032)
文摘A novel organoselenium compound,WB（1,2-[bis（1,2-benzisoselenazolone-3（2H）-ketone）]pentane） has indicated anti-tumor activity.Its pharmacokinetic data has never been determined.By using the H22 tumor bearing mouse model,the tissue distribution of WB after single and four consecutive doses（both were 120 mg/kg/d） was explored.The selenium content of the tissues was used as an indicator of WB absorption,distribution and metabolism.The selenium in the heart,liver, spleen,kidneys,lungs,stomach,pancreas,brain,colon,intestine,testes,plasma,and tumor were determined by generation atomic fluorescence spectrometry（AFS）.With single or multiple oral administration of WB,the selenium content significantly increased in the liver,stomach,colon,and intestine.The selenium content in the spleen,lungs,pancreas,testes,plasma and tumor also increased compared with the controls;but no significant changes were found in the brain and kidney.WB and its metabolites distributed predominantly in the colon,liver,stomach and intestine,which resulted in a significant increase in the selenium content in both groups.There was no observed significant accumulation of WB in the vital organs.
