Reteplase, the recombinant type of novel tissue plasminogen activator (t-PA) variant, is a promising thrombolytics in clinics. Expressed in the form of an inclusion body, reteplase consists of about 40 % of the total ...Reteplase, the recombinant type of novel tissue plasminogen activator (t-PA) variant, is a promising thrombolytics in clinics. Expressed in the form of an inclusion body, reteplase consists of about 40 % of the total intracellular proteins of Escherichia coli. The recombinant human protein disulfide isomerase (rhPDI) is used to increase the chance for the correct matching of the 18 hydrosulfide groups of the reteplase molecule in the renaturation process and it increase is the reteplase renaturation yield from 1%～2% to 15%～20% with a the purity aboue 99% and the specific activity of 5(105 IU/mg is reached. This novel method can reduce significantly the cost of production.展开更多
结肠癌的诊断及治疗一直是医疗界的难题。近年有关结肠癌差异蛋白质的研究越来越多,运用蛋白质组学技术发现癌症组织中的差异蛋白质,并研究其在癌症中的功能及作用,从而用于癌症的诊断和治疗,已成为一种新的趋势。本课题组前期通过蛋白...结肠癌的诊断及治疗一直是医疗界的难题。近年有关结肠癌差异蛋白质的研究越来越多,运用蛋白质组学技术发现癌症组织中的差异蛋白质,并研究其在癌症中的功能及作用,从而用于癌症的诊断和治疗,已成为一种新的趋势。本课题组前期通过蛋白质组学技术发现乳腺癌相关耐药蛋白2(ATP-binding cassette sub-family G member 2,ABCG2)和蛋白质二硫异构酶A2(protein disulfide-isomerase A2,PDIA2)两种结肠癌差异蛋白质,本文对这两种蛋白质在结肠癌中的表达及功能作一综述。展开更多
文摘Reteplase, the recombinant type of novel tissue plasminogen activator (t-PA) variant, is a promising thrombolytics in clinics. Expressed in the form of an inclusion body, reteplase consists of about 40 % of the total intracellular proteins of Escherichia coli. The recombinant human protein disulfide isomerase (rhPDI) is used to increase the chance for the correct matching of the 18 hydrosulfide groups of the reteplase molecule in the renaturation process and it increase is the reteplase renaturation yield from 1%～2% to 15%～20% with a the purity aboue 99% and the specific activity of 5(105 IU/mg is reached. This novel method can reduce significantly the cost of production.
文摘结肠癌的诊断及治疗一直是医疗界的难题。近年有关结肠癌差异蛋白质的研究越来越多,运用蛋白质组学技术发现癌症组织中的差异蛋白质,并研究其在癌症中的功能及作用,从而用于癌症的诊断和治疗,已成为一种新的趋势。本课题组前期通过蛋白质组学技术发现乳腺癌相关耐药蛋白2(ATP-binding cassette sub-family G member 2,ABCG2)和蛋白质二硫异构酶A2(protein disulfide-isomerase A2,PDIA2)两种结肠癌差异蛋白质,本文对这两种蛋白质在结肠癌中的表达及功能作一综述。
