盐酸多巴甲酯对映体的HPCE手性拆分

Aim:To develop an HPCE method for the chiral separation of dopa methyl ester hydrochloride enantiomers.Methods:The enantiomers were separated by HPCE on a capillary of 63.5/72.0(I/L)cm×50 μm(i.d) with the background electrolyte of 20 mmol/L sodium dihydrogen phosphate,which containing 2.5% of highly sulfated-β-cyclodextrin as the chiral resolving additive adjusted to pH 2.55 with phosphoric acid.The outlet pressure injection mode and positive voltage of 30 kV electric force were used with the cassette temperature at 25 ℃ and UV detection at 210 nm.Attempts were made to optimized the separation conditions.Results:The enantiomers were baseline separated at the optimized conditions with the resolution greater than 2.The calibration curve was linear for L-dopa methyl ester hydrochloride in the range from 10 to 4 000 μg/mL using aminobenzoic acid as internal standard.And the limit of detection was 2.0 μg/mL.Conclusion:The established HPCE method is of high performance and economy for the chiral separation.It could be used as the routine method for the chiral purity analysis of L-dopa methyl ester hydrochloride.

高效毛细管电泳法拆分5种手性药物

目的:以硫酸化-β-环糊精(S-β-CD)为手性选择剂,建立毛细管电泳法分离苯丙哌林、曲马多、联苯苄唑、利阿唑和1-苄基-3哌啶醇5种对映体药物。方法 :采用未涂壁熔融石英毛细管柱,磷酸二氢钠缓冲溶液,分离电压20 kV,考察了硫酸化-β-环糊精质量浓度、背景电解质pH值、缓冲盐浓度共3个因素对手性药物拆分的影响。结果:在最优的电泳条件下5种药物均达到基线分离,分离度分别为10.41,6.10,4.09,2.80,5.50。结论:所建立的方法适于苯丙哌林、曲马多、联苯苄唑、利阿唑和1-苄基-3哌啶醇的对映体分离。