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S14G-humanin对β-淀粉样蛋白纤维化及其细胞毒性的影响
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作者 毛妮 刘柳 +3 位作者 刘睿 李柱一 张巍 苗建亭 《中国神经免疫学和神经病学杂志》 CAS 2011年第1期10-13,共4页
目的探讨S14G-humanin(HNG)对β-淀粉样蛋白(Aβ)纤维化及其细胞毒性的影响。方法于体外将Aβ1-42和不同浓度HNG共孵育,并设空白对照,应用硫黄素-T(Th-T)荧光法和透射电镜方法,观察HNG对Aβ1-42纤维化的作用;将Aβ1-42和不同浓度HNG共... 目的探讨S14G-humanin(HNG)对β-淀粉样蛋白(Aβ)纤维化及其细胞毒性的影响。方法于体外将Aβ1-42和不同浓度HNG共孵育,并设空白对照,应用硫黄素-T(Th-T)荧光法和透射电镜方法,观察HNG对Aβ1-42纤维化的作用;将Aβ1-42和不同浓度HNG共孵育后加入培养的大鼠肾上腺髓质嗜铬瘤细胞(PC12细胞),应用MTT法测定PC12细胞活性,观察不同浓度HNG对Aβ1-42细胞毒性的拮抗作用。结果 (1)不同浓度HNG(100、200、400μmol/L)+Aβ1-42(100μmol/L)组Th-T荧光强度(241.86±8.41,188.27±4.47,112.36±5.27)均较Aβ1-42组(514.85±14.52)显著降低(P<0.01),且各组Th-T荧光强度随HNG浓度增加而降低,各组间比较有统计学差异(P<0.01)。(2)透射电镜观察表明不同浓度HNG+Aβ1-42组纤维性Aβ均较Aβ1-42组显著减少。(3)不同浓度HNG(10、20、40μmol/L)+Aβ1-42(10μmol/L)组PC12细胞活性〔(67.83±3.57)%、(79.26±3.13)%、(89.67±3.25)%〕均较Aβ1-42组〔(57.52±5.41)%〕显著增加(P<0.01),且各组细胞活性随HNG浓度增加而增加,各组间比较有统计学差异(P<0.01)。结论 HNG在体外能够有效减少纤维性Aβ1-42形成并可抑制其细胞毒性作用,提示HNG有可能成为有效防治阿尔茨海默病的新药物。 展开更多
关键词 Β-淀粉样蛋白 阿尔茨海默病 S14G-humanin 透射电镜 硫黄素-t
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Phytomedicines as potential inhibitors of β amyloid aggregation: significance to Alzheimer's disease 被引量:1
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作者 N.Satheesh Kumar N.Nisha 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第11期801-818,共18页
Throughout the history of drug development, plants have been an important source for the discovery of novel therapeutically active compounds for many diseases. The ethnopharmacological approach has provided several le... Throughout the history of drug development, plants have been an important source for the discovery of novel therapeutically active compounds for many diseases. The ethnopharmacological approach has provided several leads to identify potential new drugs from plant sources, including those for memory disorders. For the treatment of Alzheimer's disease the drug discovery focus shifted from cholinesterase inhibitors, to other targets primarily based on two key neuropathological hallmarks, namely the hyperphosphorylation of the tau protein resulting in the formation of neurofibrillary tangles(NFTs), and the increased formation and aggregation of amyloid-beta peptide(Aβ) derived from amyloid precursor protein(APP). The present article aims to provide a comprehensive literature survey of plants and their constituents that have been tested for Aβ aggregation, thus possibly relieving several features of Alzheimer's disease(AD). 展开更多
关键词 Amyloid-beta peptide Medicinal plants Thioflavin T β amyloid aggregation Plant-derived pharmaceuticals
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