Objective: To confirm the endometrial glandular epithelial and stromal cells in tissue fragments recovered from peritoneal fluid and to quantify haptoglobin expression. Design: Prospective, randomized study. Setting: ...Objective: To confirm the endometrial glandular epithelial and stromal cells in tissue fragments recovered from peritoneal fluid and to quantify haptoglobin expression. Design: Prospective, randomized study. Setting: University of Missouri-Columbia School of Medicine and Health Care System. Subject(s): Women undergoing laparoscopic surgery for diagnosis or treatment of endometriosis or for laparoscopic tubal cautery for desired sterilization. Intervention(s): Aspiration of peritoneal fluid at laparoscopic surgery. Main Outcome Measure(s): Histological confirmation of endometrial glandular epithelial and stromal cells and evaluation of haptoglobin gene expression and protein localization in shed endometrial tissue fragments recovered from peritoneal fluid. Result(s): More visible tissue fragments were found in the peritoneal fluid from women with endometriosis (n = 28/65) than from that of women without endometriosis (n = 5/34). Of these tissues, endometrial glands and stroma were histologically confirmed in about half of women with endometriosis (n = 13/28) and without endometriosis (n = 3/5). Retrogradely shed endometrial tissues recovered from peritoneal fluid robustly express haptoglobin. Conclusion(s): Haptoglobin expression by retrogradely shed endometrial tissues in peritoneal fluid supports a mechanism whereby these purported precursors of endometriotic lesions escape immune destruction. Low recovery of histologically confirmed endometrial tissue fragments from peritoneal fluid reveals the potential difficulties for using these tissues for studies modeling the pathogenesis of endometriosis.展开更多
Background: In non-obstructive azoospermic patients (NOA)-besides the mechan ical treatment, vital spermatozoa from the tissue obtained from testes by biopsy can be enzymatically prepared. Objective: To increase the s...Background: In non-obstructive azoospermic patients (NOA)-besides the mechan ical treatment, vital spermatozoa from the tissue obtained from testes by biopsy can be enzymatically prepared. Objective: To increase the sperm recovery succ es s of testicular sperm extraction (TESE), suitable for ICSI. Study design: Prospe ctive, clinical study. In 177 consecutive men who presented with clinical and la boratory data indicating NOA, tissue samples were obtained by microdissection TE SE method. Initially, mature spermatozoa were searched for by mechanical extract ion technique shredding the biopsy fractions. In cases with no spermatozoa was o bserved after maximum 30 min of initial searching under the inverted microscope, the procedure was then followed by enzymatic digestion using DNAse and collagen ase type IV. In cases of at least a mature spermatozoon could be obtained proper ly, ICSI was performed. Results: Of 177 cases with NOA, conventional mincing met hod extended with enzymatic treatment yielded successful sperm recovery for ICSI in 102 (57%). Overall in vitro tissue-processing time for patients with sperm recovery failure after 30 min of mechanical searching, was between 80 and 105 min (mean 96 ±9). Cleavage, embryo transfer and clinical pregnancy rates in mec hanical plus enzymatic TESE patients were not significantly different from those of only mechanically TESE performed patients (P >0.05). Conclusion: Combination of conventional TESE and enzymatic digestion is an effective method to recover spermatozoa suitable for ICSI. The benefit of the mincing combined with enzyme t o sperm retrieval for NOA is firstly to shorten the mechanical searching time, t hus minimizing further cellular damage as well as exposure to external condition s, and secondly to reduce the number of cases with sperm recovery failures.展开更多
文摘Objective: To confirm the endometrial glandular epithelial and stromal cells in tissue fragments recovered from peritoneal fluid and to quantify haptoglobin expression. Design: Prospective, randomized study. Setting: University of Missouri-Columbia School of Medicine and Health Care System. Subject(s): Women undergoing laparoscopic surgery for diagnosis or treatment of endometriosis or for laparoscopic tubal cautery for desired sterilization. Intervention(s): Aspiration of peritoneal fluid at laparoscopic surgery. Main Outcome Measure(s): Histological confirmation of endometrial glandular epithelial and stromal cells and evaluation of haptoglobin gene expression and protein localization in shed endometrial tissue fragments recovered from peritoneal fluid. Result(s): More visible tissue fragments were found in the peritoneal fluid from women with endometriosis (n = 28/65) than from that of women without endometriosis (n = 5/34). Of these tissues, endometrial glands and stroma were histologically confirmed in about half of women with endometriosis (n = 13/28) and without endometriosis (n = 3/5). Retrogradely shed endometrial tissues recovered from peritoneal fluid robustly express haptoglobin. Conclusion(s): Haptoglobin expression by retrogradely shed endometrial tissues in peritoneal fluid supports a mechanism whereby these purported precursors of endometriotic lesions escape immune destruction. Low recovery of histologically confirmed endometrial tissue fragments from peritoneal fluid reveals the potential difficulties for using these tissues for studies modeling the pathogenesis of endometriosis.
文摘Background: In non-obstructive azoospermic patients (NOA)-besides the mechan ical treatment, vital spermatozoa from the tissue obtained from testes by biopsy can be enzymatically prepared. Objective: To increase the sperm recovery succ es s of testicular sperm extraction (TESE), suitable for ICSI. Study design: Prospe ctive, clinical study. In 177 consecutive men who presented with clinical and la boratory data indicating NOA, tissue samples were obtained by microdissection TE SE method. Initially, mature spermatozoa were searched for by mechanical extract ion technique shredding the biopsy fractions. In cases with no spermatozoa was o bserved after maximum 30 min of initial searching under the inverted microscope, the procedure was then followed by enzymatic digestion using DNAse and collagen ase type IV. In cases of at least a mature spermatozoon could be obtained proper ly, ICSI was performed. Results: Of 177 cases with NOA, conventional mincing met hod extended with enzymatic treatment yielded successful sperm recovery for ICSI in 102 (57%). Overall in vitro tissue-processing time for patients with sperm recovery failure after 30 min of mechanical searching, was between 80 and 105 min (mean 96 ±9). Cleavage, embryo transfer and clinical pregnancy rates in mec hanical plus enzymatic TESE patients were not significantly different from those of only mechanically TESE performed patients (P >0.05). Conclusion: Combination of conventional TESE and enzymatic digestion is an effective method to recover spermatozoa suitable for ICSI. The benefit of the mincing combined with enzyme t o sperm retrieval for NOA is firstly to shorten the mechanical searching time, t hus minimizing further cellular damage as well as exposure to external condition s, and secondly to reduce the number of cases with sperm recovery failures.
