Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and...Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antiinflammatory actions. Methods The samples were analyzed on a ODS column with methanol water triethylamine (3∶97∶0 1 V/V) as a mobile phase. The flow rate was 1 0 mL·min -1 , and UV detection wavelength 205 nm. Results Linear regression equation was A=1 6920C+1 7455 (r 2=0 9999, n =5) in concentratins ranging from 20 to 120 μg·mL -1 . The recoveries were 101 2±1 46 % at 80 μg·mL -1 , 100 5±0 75% at 100 μg·mL -1 , and 100 7±1 10% at 120 μg·mL -1 , respectively, and the precisions of aloperine within or between run were from 0 80% to 1 98% ( n =5). The relative contents of aloperine in three lots of tablets were 101 59±1 38%, 98 46±0 23%, and 99 41±1 09% ( n =3). Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection.展开更多
Strips of mouse ileum were used in current study to investigate the effect of different concentrations of aspirin on their smooth muscles activities. Student＇s kymograph and glass jacket organ bath were used for reco...Strips of mouse ileum were used in current study to investigate the effect of different concentrations of aspirin on their smooth muscles activities. Student＇s kymograph and glass jacket organ bath were used for recording ilum smooth muscles contractions. The recording of the muscles contractions showed regular autorhythmic pattern, and this contraction was enhanced by both ACh （acetylcholine） and KCI （potassium chloride）. This normal contraction and that induced by ACh and KCI mainly depended on the extracellular calcium in their tension and maintaining. Aspirin exerted different effects on ileal smooth muscles contractions depending upon concentrations. Low concentrations 2-40 mM had an stimulatory effects, while high concentrations 60-200 mM had an inhibitory effects, which led to relaxation. Ca^＋2 free solution abolished muscles response to aspirin with the concentrations caused contractions. Aspirin in tum led to continuation of acetylcholine induced contractions.展开更多
文摘Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antiinflammatory actions. Methods The samples were analyzed on a ODS column with methanol water triethylamine (3∶97∶0 1 V/V) as a mobile phase. The flow rate was 1 0 mL·min -1 , and UV detection wavelength 205 nm. Results Linear regression equation was A=1 6920C+1 7455 (r 2=0 9999, n =5) in concentratins ranging from 20 to 120 μg·mL -1 . The recoveries were 101 2±1 46 % at 80 μg·mL -1 , 100 5±0 75% at 100 μg·mL -1 , and 100 7±1 10% at 120 μg·mL -1 , respectively, and the precisions of aloperine within or between run were from 0 80% to 1 98% ( n =5). The relative contents of aloperine in three lots of tablets were 101 59±1 38%, 98 46±0 23%, and 99 41±1 09% ( n =3). Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection.
文摘Strips of mouse ileum were used in current study to investigate the effect of different concentrations of aspirin on their smooth muscles activities. Student＇s kymograph and glass jacket organ bath were used for recording ilum smooth muscles contractions. The recording of the muscles contractions showed regular autorhythmic pattern, and this contraction was enhanced by both ACh （acetylcholine） and KCI （potassium chloride）. This normal contraction and that induced by ACh and KCI mainly depended on the extracellular calcium in their tension and maintaining. Aspirin exerted different effects on ileal smooth muscles contractions depending upon concentrations. Low concentrations 2-40 mM had an stimulatory effects, while high concentrations 60-200 mM had an inhibitory effects, which led to relaxation. Ca^＋2 free solution abolished muscles response to aspirin with the concentrations caused contractions. Aspirin in tum led to continuation of acetylcholine induced contractions.
