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表皮生长因子和碱性成纤维细胞生长因子联合应用于深度褥疮的护理研究 被引量:4
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作者 翁秀珍 徐俊赐 +2 位作者 郑秀先 魏立繁 罗儿雪 《护理研究》 2005年第7期1165-1166,共2页
[目的]探讨表皮生长因子和碱性成纤维细胞生长因子联合应用于深度褥疮的护理效果。[方法]选择褥疮45例处,其中Ⅱ期褥疮19例处、Ⅲ期21例处、Ⅳ期5例处,随机分为实验组和对照组,实验组将表皮生长因子和碱性成纤维细胞生长因子联合喷洒于... [目的]探讨表皮生长因子和碱性成纤维细胞生长因子联合应用于深度褥疮的护理效果。[方法]选择褥疮45例处,其中Ⅱ期褥疮19例处、Ⅲ期21例处、Ⅳ期5例处,随机分为实验组和对照组,实验组将表皮生长因子和碱性成纤维细胞生长因子联合喷洒于褥疮创面,结合微波治疗;对照组用抗生素药液湿敷,予红外线局部照射,观察创面修复情况及愈合速度。[结果]实验组创面愈合速度为0.1mm/d~0.3mm/d,对照组为0.07mm/d~0.15mm/d,差异有统计学意义(P<0.05);实验组有效率高于对照组。[结论]表皮生长因子和碱性成纤维细胞生长因子联合应用于深度褥疮的治疗,疮面愈合效果好于传统的抗生素湿敷及红外线照射法。 展开更多
关键词 褥疮 表皮生长因子 碱性成纤 维细胞生长因子 疗效
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三七总皂甙对脑出血大鼠前脑碱性成纤维细胞生长因子受体影响的实验研究 被引量:13
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作者 张俊敏 朱培纯 《中国中医基础医学杂志》 CAS CSCD 2000年第10期30-33,共4页
目的 :研究三七总皂甙对脑出血大鼠脑内碱性成纤维细胞生长因子 (FGFR1 )表达的影响及可能机制。方法 :用免疫组化方法观察胶原酶诱导的脑出血大鼠脑内 FGFR1在给药组和对照组的表达变化。结果 :脑出血后脑内 FGFR1主要在病灶周围、扣... 目的 :研究三七总皂甙对脑出血大鼠脑内碱性成纤维细胞生长因子 (FGFR1 )表达的影响及可能机制。方法 :用免疫组化方法观察胶原酶诱导的脑出血大鼠脑内 FGFR1在给药组和对照组的表达变化。结果 :脑出血后脑内 FGFR1主要在病灶周围、扣带皮质、海马缰核表达 ,阳性细胞主要有神经元 ,给药组比对照组表达明显增加。结论 :三七总皂甙可通过上调 b FGFR1的表达 ,从而促进脑出血后脑内神经元的存活及损伤修复。 展开更多
关键词 碱性成纤堆细胞生长因子受体 脑出血 免疫组化 大鼠
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碱性成纤维生长因子与神经再生的研究进展
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作者 王昱 朱宇熹 孔祥英 《重庆医学》 CAS CSCD 2004年第3期462-463,共2页
关键词 碱性成纤雏生长因子 神经 再生
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血管形成与膀胱癌分级及复发相关性研究 被引量:1
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作者 王亮 陈昭颉 +4 位作者 王庆堂 曹文峰 简燚 刘祥丹 汪俊超 《西南军医》 2004年第6期1-3,共3页
目的 观察膀胱癌微血管密度 (MVD)及碱性成纤维细胞生长因子 (bFGF)与膀胱癌分级及复发的关系。方法 本组膀胱移行细胞癌患者 6 2例 ,年龄 36~ 82岁 ,平均 6 5岁。采用免疫组织化学染色CD34观察膀胱癌组织中MVD以及bFGF的表达情况。... 目的 观察膀胱癌微血管密度 (MVD)及碱性成纤维细胞生长因子 (bFGF)与膀胱癌分级及复发的关系。方法 本组膀胱移行细胞癌患者 6 2例 ,年龄 36~ 82岁 ,平均 6 5岁。采用免疫组织化学染色CD34观察膀胱癌组织中MVD以及bFGF的表达情况。结果 G1级、G2级、G3膀胱癌中bFGF阳性率分别为 33.3%、4 8%、73.7% ,其中 ,G1级与G3级膀胱癌间差异有统计学意义 (P <0 .0 5 )。膀胱癌复发组及未复发组bFGF阳性率分别为 73.1%、36 .1%(P <0 .0 5 )。G1级、G2级、G3膀胱癌MDV分别为 4 4 .3± 11.6、5 3.5± 10 .6、71.9± 15 .3,G1级与G3级膀胱癌间差异有统计学意义 (P <0 .0 5 )。膀胱癌复发组及未复发组MDV分别为 4 1.7± 10 .9、77.2± 15 .3(P <0 .0 5 )。结论 膀胱癌bFGF表达及MVD与其分级及肿瘤复发相关 。 展开更多
关键词 膀胱肿瘤 血管形 碱性成纤雏细胞生长因子
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急性心肌梗死血管生成和bFGF表达
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作者 孙立军 刘莹 +3 位作者 宦怡 赵海涛 葛雅丽 郜发宝 《心脏杂志》 CAS 2004年第4期305-307,共3页
目的 :探讨心肌缺血后血管生成和碱性成纤维生长因子 (basic fibroblast growth factor,b FGF)表达的关系。方法 :2 4只犬建立急性心肌梗死 (myocardial infarction,MI)模型后随机分成对照组 (MI区注射生理盐水 15 m l)和实验组 (MI区注... 目的 :探讨心肌缺血后血管生成和碱性成纤维生长因子 (basic fibroblast growth factor,b FGF)表达的关系。方法 :2 4只犬建立急性心肌梗死 (myocardial infarction,MI)模型后随机分成对照组 (MI区注射生理盐水 15 m l)和实验组 (MI区注射 b FGF5 0 mg与生理盐水的混合液 15 ml)。每组观察 4个不同时间点 (术后第 1、3、10和 17天 ) ,每个小组 3只犬。免疫组织化学方法检测各组心肌细胞中 b FGF的表达及微血管数量。结果 :除第 1天外各个时间点的微血管数量实验组比对照组显著增多 (对照组和实验组分别为 :第 3天 :92± 12、14 7± 12 ;第 10天 :12 5± 12、183± 14 ;第 17天 :12 5± 15、2 2 4± 2 0 ;单位 :个 /视野 ) ;心肌缺血区治疗组 b FGF的表达增多 (对照组和实验组分别为 :第 1天 :15± 2、35± 7;第 3天 :34± 4、6 4± 6 ;第 10天 :5 5± 9、79± 4 ;第 17天 :2 1± 5、37± 13)。结论 :局部心肌内注射 b FGF有促进 MI区域毛细血管形成的作用 ;心肌缺血区 b FGF的表达与 MI区域毛细血管的密度密切相关。 展开更多
关键词 心肌梗塞 碱性成纤雏生长因子 心肌缺血
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bFGF对局灶性脑缺血大鼠脑组织GRP78表达的影响 被引量:5
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作者 邢雪松 吕威力 +1 位作者 赵海 姜海波 《解剖学研究》 CAS 2012年第4期290-292,302,F0004,共5页
目的研究大鼠脑缺血再灌注后对葡萄糖调节蛋白78(glucose-regulated protein78,GRP78)表达的影响,探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对脑组织缺血再灌注神经元GRP78的调节作用及机制。方法应用线栓法制... 目的研究大鼠脑缺血再灌注后对葡萄糖调节蛋白78(glucose-regulated protein78,GRP78)表达的影响,探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对脑组织缺血再灌注神经元GRP78的调节作用及机制。方法应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞2h再灌注损伤12h,采用TUNEL法、免疫组化检测海马及皮质内神经元凋亡和GRP78的表达。结果 sham组,海马及皮质偶见凋亡细胞,皮质及海马神经元内少见GRP78免疫反应阳性细胞;I/R组,海马及皮质神经元凋亡增加,缺血再灌注损伤后皮质及海马神经元内GRP78阳性表达明显高于假手术组。bFGF组,海马及皮质神经元凋亡减少,皮质及海马神经元内GRP78表达较I/R组明显增加。结论 bFGF显著减少缺血神经元凋亡,上调脑缺血诱导的GRP78表达,对脑缺血再灌注海马及皮质神经元的具有保护作用。 展开更多
关键词 脑缺血 碱性成纤组细胞生长因子 葡萄糖调节蛋白78 海马 凋亡
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人羊膜上皮细胞培养液抑制角膜新生血管的实验研究 被引量:2
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作者 李彬斌 杨筱曦 +2 位作者 周清 何艳花 陈剑 《中华实验眼科杂志》 CAS CSCD 北大核心 2012年第5期408-413,共6页
背景角膜新生血管(CNV)是一种常见的眼部病变,研究其发病机制及其抑制剂是眼科研究的热点和难点。目的研究人羊膜上皮细胞(AECs)培养液对CNV的抑制作用及机制。方法消化法培养及鉴定人AECs,并收集培养液,酶联免疫吸附试验(ELIS... 背景角膜新生血管(CNV)是一种常见的眼部病变,研究其发病机制及其抑制剂是眼科研究的热点和难点。目的研究人羊膜上皮细胞(AECs)培养液对CNV的抑制作用及机制。方法消化法培养及鉴定人AECs,并收集培养液,酶联免疫吸附试验(ELISA)法检测色素上皮衍生因子(PEDF)和白细胞介素1受体拮抗剂(IL-1Ra)在培养液中的质量浓度。兔角膜上皮细胞分离后分别用无血清DMEM培养基、人AECs培养液、混合培养液(DMEM+人AECs培养液)培养48h,采用实时定量聚合酶链反应(QRT—PCR)法检测不同培养液培养的角膜上皮细胞中血管内皮生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)的表达。用含质量分数10%胎牛血清的DMEM培养基培养人脐静脉血管内皮细胞(UVECs),并分别加入无血清DMEM、混合培养液和人AECs培养液,划痕法和细胞计数试剂盒8(CCK8)法检测各组培养液对人UVECs迁移的影响。分别在上述3种培养基中加入终质量浓度为50txg/L的bFGF,CCK8法检测各培养液中人UVECs的增生情况。在原子力显微镜(AFM)下观察人AECs培养液对人UVECs超微结构的影响。结果人羊膜培养和传代细胞角蛋白免疫组织化学染色阳性证实为人AECs。与无血清DMEM组相比,人AECs培养液组的兔角膜上皮细胞VEGFmRNA(1.00+0.22VS.2.98_+0.46)及bFGFmRNA(1.00+0.36VS.2.55-+0.48)的表达均明显下降,差异均有统计学意义(p_-0.001、0.002);培养后不同时间人AECs培养液组人UVECs的增生吸光度(A)值明显下降,差异均有统计学意义(P〈0.05),人UVECs的迁移率下降,差异有统计学意义(P〈0.05)。AFM观察见人AECs培养液组的血管内皮细胞膜粗糙、表面颗粒紊乱,细胞间连接及伪足减少。ELISA法检测人AECs培养液中PEDF的质量浓度为(70.41±0.68)Ixg/L,IL-1Ra的质量浓度为(153.56±0.36)-s/L,无血清DMEM组中未检出。结论人AECs培养液可抑制角膜上皮VEGF及bFGF的表达,抑制血管内皮细胞的增生和迁移,细胞结构和功能改变,这可能是其抑制CNV的机制之一。 展开更多
关键词 人羊膜上皮细胞 角膜新生血管 血管内皮生长因子 人脐静脉血管内皮细胞 碱性成纤 维细胞生长因子 超微结构
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Involvement of MAPK/ERK kinase-ERK pathway in exogenous bFGF-induced Egr-1 binding activity enhancement in anoxia-reoxygenation injured astrocytes
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作者 刘颖 陆锦标 +1 位作者 陈琦 叶诸榕 《Neuroscience Bulletin》 SCIE CAS CSCD 2007年第4期221-228,共8页
Objective Intravenous administration of basic fibroblast growth factor (bFGF) is effective to reduce the volume of cerebral infract due to ischemia. This study was designed to investigate the molecular mechanism, es... Objective Intravenous administration of basic fibroblast growth factor (bFGF) is effective to reduce the volume of cerebral infract due to ischemia. This study was designed to investigate the molecular mechanism, especially the signal transduction pathways, involved in this protective role of bFGF. Methods Anoxia-reoxygenation treated atrocytes were used to study the role of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MAPK/ERK kinase, MEK)-ERK signaling pathway after exogenous bFGF administration by Western blot. Electrophoretic mobile shift assay was used to detect the binding activity of early growth response factor-1 (Egr-1), an important transcription factor for endogenous bFGF. Results bFGF could protect some signal transduction proteins from the oxygen-derived free radicals induced degradation. ERK1/2 was activated and involved in Egr-1 binding activity enhancement induced by exogenous bFGF. Conclusion MEK-ERK MAPK cascade may be an important signal transduction pathway contributed to bFGF induced enhancement of Egr-1 binding activity in anoxia-reoxygenation injured astrocytes. 展开更多
关键词 extracellular signal-regulated kinase mitogen-activated protein kinase free radicals fibroblast growth factor 2 early growth response protein 1 ASTROCYTE
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Study on Agrobacterium tumefaciens-mediated Transformation of Brassica campestris L. with Fusion Gene Ycoil-bFGF
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作者 徐岩 肖艳双 +4 位作者 杜金霞 汪洪 郑伟 李营 庞实锋 《Agricultural Science & Technology》 CAS 2009年第4期31-36,共6页
[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF wa... [ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed ( Brassica campestris L. ) by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows: explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds. 展开更多
关键词 Basic fibroblast growth factor (bFGF) Plant bioreactor Oil-body system Agrobacterium tumefaciens Brassica campestris L.
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Expression of fibroblast activation protein in human pancreatic adenocarcinoma and its clinicopathological significance 被引量:31
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作者 Min Shi Dang-Hui Yu +5 位作者 Ying Chen Chen-Yan Zhao Jing Zhang Qing-Hua Liu Can-Rong Ni Ming-Hua Zhu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第8期840-846,共7页
AIM: To examine fibroblast activation protein (FAP) expression in pancreatic ductal adenocarcinoma (PDAC) and to analyze its relationship with the clinicopathology of PDAC. METHODS: FAP expression was examined in 134 ... AIM: To examine fibroblast activation protein (FAP) expression in pancreatic ductal adenocarcinoma (PDAC) and to analyze its relationship with the clinicopathology of PDAC. METHODS: FAP expression was examined in 134 PDAC specimens by immunohistochemistry, and in four pancreatic cancer cell lines (SW1990, Miapaca-2, AsPC-1 and BxPC-3) by Western blotting assay. We also analyzed the association between FAP expression in PDAC cells and the clinicopathology of PDAC patients. RESULTS: The results showed that the FAP was expressed in both stromal fibroblast cells (98/134, 73.1%) and carcinoma cells (102/134, 76.1%). All 4 pancreatic cancer cell lines expressed FAP protein at different levels. Protein bands corresponding to the proteolytically active 170-kDa seprase dimer and its88-kDa seprase subunit were identif ied. Higher FAP expression in carcinoma cells was associated with tumor size (P < 0.001), fi brotic focus (P = 0.003), perineural invasion (P = 0.009) and worse clinical outcome (P = 0.0085). CONCLUSION: FAP is highly expressed in carcinoma cells and f ibroblasts in PDAC tissues, and its expression is associated with desmoplasia and worse prognosis. 展开更多
关键词 Pancreatic ductal adenocarcinoma Cancer-associated fibroblasts Fibroblast activation protein PROGNOSIS
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Toxicarioside A inhibits SGC-7901 proliferation,migration and invasion via NF-κB/bFGF signaling 被引量:8
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作者 Jun-Li Guo Shao-Jiang Zheng +10 位作者 Yue-Nan Li Wei Jie Xin-Bao Hao Tian-Fa Li Li-Ping xia Wen-Li Mei Feng-Ying Huang Yue-QiongKong Qi-Yi He, Kun Yang Guang-Hong Tan Hao-Fu Dai 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第14期1602-1609,共8页
AIM:To investigate the inhibitory role of toxicarioside A on the gastric cancer cell line human gastric cancer cell line(SGC-7901) and determine the underlying molecular mechanism.METHODS:After SGC-7901 cells were tre... AIM:To investigate the inhibitory role of toxicarioside A on the gastric cancer cell line human gastric cancer cell line(SGC-7901) and determine the underlying molecular mechanism.METHODS:After SGC-7901 cells were treated with toxicarioside A at various concentrations(0.5,1.5,4.5,9.0 μg/mL) for 24 h or 48 h,cell viability was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl2H-tetrazolium bromide assay,and the motility and invasion of tumor cells were assessed by the Transwell chamber assay.Immunofluorescence staining,reverse transcription polymerase chain reaction and Western blotting were performed to detect the expression of basic fibroblast growth factor(bFGF) and fibroblast growth factor receptor-1(FGFR1),and nuclear factorkappa B(NF-κB) activation was examined by electrophoretic mobility shift assay.RESULTS:The results showed that toxicarioside A was capable of reducing cell viability,inhibiting cell growth,and suppressing cell migration and invasion activities in a time-and dose-dependent manner in SGC-7901 cells.Further analysis revealed that not only the expression of bFGF and its high-affinity receptor FGFR1 but also the NF-κB-DNA binding activity were effectively blocked by toxicarioside A in a dose-dependent manner compared with the control group(P < 0.05 or P < 0.01).Interestingly,application of the NF-κB specific inhibitor,pyrrolidinedithiocarbamate(PDTC),to SGC-7901 cells significantly potentized the toxicarioside A-induced down-regulation of bFGF compared with the control group(P < 0.05).CONCLUSION:These findings suggest that toxicarioside A has an anti-gastric cancer activity and this effect may be achieved partly through down-regulation of NF-κB and bFGF/FGFR1 signaling. 展开更多
关键词 Anti-migration ANTI-PROLIFERATION Basic fibroblast growth factor Gastric cancer Nuclear factorkappa B Toxicarioside A
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Establishment and Characterization of a New Cell Line from the Kidney of Spotted Halibut Verasper variegates 被引量:5
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作者 WANG Xianli CHEN Songlin +2 位作者 SHA Zhenxia FAN Tingjun WANG Na 《Journal of Ocean University of China》 SCIE CAS 2010年第2期162-168,共7页
A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium (MEM) supplemented with fetal bovine serum (FBS... A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium (MEM) supplemented with fetal bovine serum (FBS) and 10 ng ml4 basic fibroblast growth factor (bFGF). Cell morphology from primary culture and subculture was observed continuously by microscopy. The SHK cell line consisted predominantly of fibroblast-like cells. The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃. The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%. The doubling time of the cells was determined to be 44.8 h. Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number (2n=46). The cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies. The cells were infected by lymphosystis disease virus (LCDV) and found to be susceptible to the virus in cytopathic effect (CPE) observation. The infection was confirmed by PCR and electron microscopy experiments, which proved the existence of the viral particles in the cytoplasm of the virus-infected cells. 展开更多
关键词 cell line spotted halibut Verasper variegates SHK cell line TRANSFECTION LCDV
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Feeder-free maintenance of hESCs in mesenchymal stem cell-conditioned media: distinct requirements for TGF-β and IGF-Ⅱ 被引量:7
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作者 Rosa Montes Gertrudis Ligero Laura Sanchez Purificacidn Catalina Teresa de la Cueva Ana Nieto Gustavo J Melen Ruth Rubio Javier Garcia-Castro Clara Bueno Pablo Menendez 《Cell Research》 SCIE CAS CSCD 2009年第6期698-709,共12页
A paracrine regulation was recently proposed in human embryonic stem cells (hESCs) grown in mouse embryonic fibroblast (MEF)-conditioned media (MEF-CM), where hESCs spontaneously differentiate into autologous fi... A paracrine regulation was recently proposed in human embryonic stem cells (hESCs) grown in mouse embryonic fibroblast (MEF)-conditioned media (MEF-CM), where hESCs spontaneously differentiate into autologous fibroblastlike cells to maintain culture homeostasis by producing TGF-β and insulin-like growth factor-lI (IGF-Ⅱ) in response to basic fibroblast growth factor (bFGF). Although the importance of TGF-β family members in the maintenance of pluripotency of hESCs is widely established, very little is known about the role of IGF-Ⅱ. In order to ease hESC cul- ture conditions and to reduce xenogenic components, we sought (i) to determine whether hESCs can be maintained stable and pluripotent using CM from human foreskin fibroblasts (HFFs) and human mesenchymal stem cells (hM- SCs) rather than MEF-CM, and (ii) to analyze whether the cooperation of bFGF with TGF-β and IGF-Ⅱ to maintain hESCs in MEF-CM may be extrapolated to hESCs maintained in allogeneic mesenchymal stem cell (MSC)-CM and HFF-CM. We found that MSCs and HFFs express all FGF receptors (FGFR1-4) and specifically produce TGF-β in response to bFGF. However, HFFs but not MSCs secrete IGF-Ⅱ. Despite the absence of IGF-Ⅱ in MSC-CM, hESC pluripotency and culture homeostasis were successfully maintained in MSC-CM for over 37 passages. Human ESCs derived on MSCs and hESCs maintained in MSC-CM retained hESC morphology, euploidy, expression of surface markers and transcription factors linked to pluripotency and displayed in vitro and in vivo multilineage developmental potential, suggesting that IGF-Ⅱ may be dispensable for hESC pluripotency. In fact, IGF-Ⅱ blocking had no effect on the homeostasis of hESC cultures maintained either on HFF-CM or on MSC-CM. These data indicate that hESCs are successfully maintained feeder-free with IGF-Ⅱ-lacking MSC-CM, and that the previously proposed paracrine mechanism by which bFGF cooperates with TGF-β and IGF-Ⅱ in the maintenance of hESCs in MEF-CM may not be fully extrapolated to hESCs maintained in CM from human MSCs. 展开更多
关键词 TGF-Β IGF-Ⅱ bFGF human ESCs mesenchymal stem cells conditioned media feeder-free
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Inhibition of tumor angiogenesis by TTF1 from extract of herbal medicine 被引量:11
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作者 Chao Liu Xiao-Wan Li +3 位作者 Li-Min Cui Liang-Chang Li Li-Yan Chen Xue-Wu Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第44期4875-4882,共8页
AIM:To study the inhibition of tumor angiogenesis by 5,2,4'-trihydroxy-6,7,5'-trimethoxyflavone(TTF1) isolated from an extract of herbal medicine Sorbaria sorbifolia.METHODS:Angiogenic activity was assayed usi... AIM:To study the inhibition of tumor angiogenesis by 5,2,4'-trihydroxy-6,7,5'-trimethoxyflavone(TTF1) isolated from an extract of herbal medicine Sorbaria sorbifolia.METHODS:Angiogenic activity was assayed using the chick embryo chorioallantoic membrane(CAM) method.Microvessel density(MVD) was determined by staining tissue sections immunohistochemically for CD34 using the Weidner capillary counting method.The mRNA and protein levels of vascular endothelial growth factor(VEGF),vascular endothelialgrowth factor receptor 2(VEGFR2,Flk-1/KDR),basic fibroblast growth factor(bFGF),cyclo-oxygenase(COX)-2 and hypoxia-inducible factor(HIF)-1α were detected by quantitative real-time polymerase chain reaction and Western blotting analysis.RESULTS:The TTF1 inhibition rates for CAM were 30.8%,38.2% and 47.5% with treatment concentrations of 25,50 and 100 μg/embryo × 5 d,respectively.The inhibitory rates for tumor size were 43.8%,49.4% and 59.6% at TTF1 treatment concentrations of 5,10,and 20 μmol/kg,respectively.The average MVD was 14.2,11.2 and 8.5 at treatment concentrations of 5 μmol/kg,10 μmol/kg and 20 μmol/kg TTF1,respectively.The mRNA and protein levels of VEGF,KDR,bFGF,COX-2 and HIF-1α in mice treated with TTF1 were significantly decreased.CONCLUSION:TTF1 can inhibit tumor angiogenesis,and the mechanism may be associated with the down-regulation of VEGF,KDR,bFGF,HIF-1α and COX-2. 展开更多
关键词 Chinese herbal medicine Sorbaria sorbifolia TTF1 Inhibition Tumor angiogenesis
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Development of a Synthetic Medium for the in Vitro Culture of Bovine Embryos
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作者 Moreno D. Neira A. +5 位作者 Dubreil L. Liegeois L. Destrumelle S. Briand-Amirat L. Bencharif D. Tainturier D. 《Journal of Agricultural Science and Technology(A)》 2014年第8期679-685,共7页
The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines (GF-CYK): insulin-like growth factorl (IGF-Ⅰ), insulin-like growth f... The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines (GF-CYK): insulin-like growth factorl (IGF-Ⅰ), insulin-like growth factorⅡ (IGF-Ⅱ), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), granulocyte-macrophage colony stimulating factor (GM-CSF) and transforming growth factor beta Ⅰ (TGF-β1) + hyaluronan (HA) + recombinant albumin (RA). The embryos were cultured in synthetic oviduct fluid (SOF) supplemented with: treatment 1 (T1): bovine serum albumin (BSA) + insulin, transferrin and selenium (ITS) (control); or treatment 2 (T2): GF-CYK + HA + RA. The blastocyst rates were not significantly different between TI and T2, at seven days post fertilization (dpf) (28.9% ± 2.4% and 31.8% ±2.2%), and at 8 dpf (36.5% ±2.4% and 39.1% ±1.9%), respectively (P 〉 0.05). The total cell number (TCN) was significantly higher with T2 than that with T1 at 7 dpf(164.9 ±5.3 and 149.7 ±4.0) and 8 dpf (182.7 ±6.4 and 165.0 ±5.5) (P 〈 0.05). The blastocyst diameter obtained with T2 was significantly greater (P 〈 0.05) than with T1 at 7 dpf (173.3 μm ±4.9 μm and 157.2μm ±4.1 μm, respectively), however, no significant differences were observed at 8 dpf (190.3 μm 5.2 μm and 179.7 μm ± 5.3 μm, respectively). In conclusion, the synthetic medium (T2) shows a comparable development rate to the control medium and improves the blastocyst diameter and the TCN. 展开更多
关键词 Growth factors CYTOKINES synthetic media.
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Expression of VEGF-C and b-FGF in Lung Cancer and Its Relationship with Cancer Progress
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作者 Liu Yan Ma WenHao Ji Liang 《International English Education Research》 2014年第2期78-81,共4页
Objective: to observe expression of vascular endothelial growth factor-C (VEGF-C) and alkaline fibroblast growth factor (b-FGF) in tissues of lung cancer, and its relationship with cancer metastasis.Methods: to ... Objective: to observe expression of vascular endothelial growth factor-C (VEGF-C) and alkaline fibroblast growth factor (b-FGF) in tissues of lung cancer, and its relationship with cancer metastasis.Methods: to adopt immunohistochemical methods, analysis of 60 cases of lung tissue expression of VEGF-C and b-FGF in the situation.Result: positive rates of VEGF-C and b-FGF in lung cancer are respectively 56.67% and 63.33%; expression of VEGF-C and b-FGF in lung cancer is not related to pathological grades, pathologic stages or ages of patients (P 〉 0.05),but closely related to TNM stages and existence of lymph node metastasis (P 〈 0.01). IMVD in center of lung cancer tissues is obviously higher than surrounding area, with significant differences (P 〈 0.01). Conclusion: expression of VEGF-C and b-FGF is related to lung cancer progress. 展开更多
关键词 VEGF-C B-FGF lung cancer progress.
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Healing of chronic cutaneous wounds by topical treatment with basic fibroblast growth factor 被引量:43
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作者 付小兵 郭振荣 +2 位作者 盛志勇 沈祖尧 张明良 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第3期331-335,共5页
OBJECTIVE: To evaluate the safety and efficacy of topical application of recombinant bovine basic fibroblast growth factor (rbFGF) on the healing of chronic cutaneous wounds. METHODS: Twenty-eight patients with thirty... OBJECTIVE: To evaluate the safety and efficacy of topical application of recombinant bovine basic fibroblast growth factor (rbFGF) on the healing of chronic cutaneous wounds. METHODS: Twenty-eight patients with thirty-three chronic cutaneous wounds resulting from trauma, diabetes mellitus, pressure sore and radiation injuries were enrolled in this prospective, open-label crossover trial. Prior to treatment with rbFGF, all wounds failed to heal with conventional therapies within 4 weeks. All wounds were locally treated with rbFGF at a dose of 150 AU/cm(2). Healing time and the quality of wounds were used to evaluate the efficacy of the treatment. RESULTS: Healing of all chronic wounds was expedited. During the study, eighteen wounds completely healed within 2 weeks, four healed within 3 weeks, and another eight completely healed within 4 weeks. Only three wounds failed to heal within 4 weeks, but healed at 30, 40 and 42 days after treatment with rbFGF. Thus, compared with conventional therapies, the effective rate of rbFGF treatment within 4 weeks was 90.9%. Histological assessment showed more abundant capillary sprouts or tubes and that fibroblasts were differentiated in wounds treated with rbFGF. No adverse side effects related to basic fibroblast growth factor were observed. CONCLUSIONS: Our results indicate that rbFGF could be used to accelerate healing in chronic wounds. It is our belief that this may be a more effective method of chronic wound management. 展开更多
关键词 Wound Healing Administration Topical Adolescent Adult Aged Aged 80 and over Child Chronic Disease Female Fibroblast Growth Factor 2 Humans Male Middle Aged Recombinant Proteins Research Support Non-U.S. Gov't Skin Skin Ulcer
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Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells 被引量:1
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作者 申屠形超 姚克 +2 位作者 孙朝晖 徐雯 吴仁毅 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第2期268-271,156,共4页
OBJECTIVE: To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correl... OBJECTIVE: To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correlation with age. METHODS: Enucleated eyes were subjected to immunostaining for bFGF protein. Human cataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry. RESULTS: bFGF protein was found in various human ocular tissues. bFGF stimulated human cataract LEC proliferation, and there was an age-related decrease in responsiveness of human cataract LECs to bFGF (P 展开更多
关键词 Adolescent Adult Age Factors CATARACT CHILD Child Preschool Epithelial Cells Fibroblast Growth Factor 2 Humans Immunohistochemistry INFANT Infant Newborn Lens Crystalline Middle Aged Proliferating Cell Nuclear Antigen Research Support Non-U.S. Gov't
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Permanent myopathy caused by mutation of SCN4A Metl592Val:Observation on myogenesis in vitro and on effect of basic fibroblast growth factor on the muscle
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作者 冯昱 王宏 +1 位作者 罗晓光 任艳 《Neuroscience Bulletin》 SCIE CAS CSCD 2009年第2期61-66,共6页
Objective The present study is to observe in vitro the proliferation ability of the muscle cells from permanent myopathy (PM) patients of nomokalaemic periodic paralysis (normKPP), which is caused by mutations of ... Objective The present study is to observe in vitro the proliferation ability of the muscle cells from permanent myopathy (PM) patients of nomokalaemic periodic paralysis (normKPP), which is caused by mutations of Metl592Val in the skeletal muscle voltage gated sodium channel (SCN4A) gene on chromosome 17q23.1. We also evaluate the possible effect of the foreign basic fibroblast growth factor (bFGF) in preventing and curing PM. Methods The gastrocnemius muscle cells were taken from two male patients with PM of the same Chinese family with Metl592Val mutation of SCN4A, determined by gene screening. Four male patients suffering from the skeletal injury without PM were taken as control. All preparations were protogenerationally cultured in vitro. Proliferation of the cultured preparations was measured by MTT. Activities of the lactic dehydrogenase (LDH), creatine kinase (CK), and protein content in these cells were also detected. The effects of bFGF with different doses (10 ng/mL, 20 ng/mL, 40 ng/mL, 80 ng/mL, 120 ng/mL and 160 ng/mL) on the above mentioned parameters were also evaluated. Results Cells from both PM and control subjects were successfully cultured in vitro. The cultivation of the muscle cells from PM patients in vitro was not yet seen. Results indicated the obvious stimulation of bFGF on cell proliferation, activities of LDH and CK, protein synthesis, in a dose dependent manner. The optimal dose of bFGF was 120 ng/mL (P〈0.05), beyond which greater dose caused a less effect. The effect of bFGF on 160 ng/mL was stronger than that on 80 ng/mL, but there was no significant difference (P〉0.05). Conclusion Myoblastic cells from patients with PM had a weaker ability of developing into the myotubules, thus they were unable to perform effective regeneration, which resulted in a progressive necrosis. The exogenous bFGF could promote the division and proliferation of the muscle cells in vitro. These results shield a light on bFGF's potential role in preventing and treating PM. 展开更多
关键词 SCN4A permanent myopathy cell culture basic fibroblast growth factor
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Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model 被引量:23
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作者 SHI Qin GAO Wei +7 位作者 HAN XingLong ZHU XueSong SUN Jie XIE Fang HOU XiangLin YANG HuiLin DAI JianWu CHEN Liang 《Science China(Life Sciences)》 SCIE CAS 2014年第2期232-240,共9页
Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by ... Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by itself was difficult to meet the need of SCI functional recovery.The basic fibroblast growth factor(bFGF)administration significantly promotes functional recovery after organ injuries.Here,using a rat model of T9 hemisected SCI,we aimed at assessing the repair capacity of implantation of collagen scaffold(CS)modified by collagen binding bFGF(CBD-bFGF).The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI.The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group,when evaluated by the 21-point Basso-Beattie-Bresnahan(BBB)score and footprint analysis.Both hematoxylin and eosin(H&E)and immunohistochemical staining of neurofilament(NF)and glial fibrillary acidic protein(GFAP)demonstrated that fibers were guided to grow through the implants.These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery. 展开更多
关键词 collagen scaffold basic fibroblast growth factor spinal cord injury nerve regeneration
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