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大鼠肝/骨/肾型碱性磷酸酶部分cDNA的定序克隆 被引量:1
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作者 陈筠 史俊南 肖明振 《牙体牙髓牙周病学杂志》 CAS 1991年第1期38-41,65,共5页
碱性磷酸酶(ALP)是较为肯定的参与钙化的酶类。牙髓组织合有ALP,性质与肝/骨/肾型(L/B/K)ALP相似。采用基因工程技术,研究细胞中ALP基因表达情况,有助于从分子水平上了解药物的作用机理。由于从细胞全基因组文库中获得ALP基因的工程量大... 碱性磷酸酶(ALP)是较为肯定的参与钙化的酶类。牙髓组织合有ALP,性质与肝/骨/肾型(L/B/K)ALP相似。采用基因工程技术,研究细胞中ALP基因表达情况,有助于从分子水平上了解药物的作用机理。由于从细胞全基因组文库中获得ALP基因的工程量大,耗时耗力,因此,我们以体外合成的大鼠L/B/KALPcDNA的高保守性的ALP活性区的2段核酸序列为引物,采用反转录PCR和定序克隆获得大鼠L/B/KALP部分cDNA的分子克隆。 展开更多
关键词 碱性碱酸酶 CDNA 聚合链反应 分子克隆
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179例9~12岁儿童碱性磷酸酶测定结果分析
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作者 潘秀琴 伊惠霞 +2 位作者 边红星 葛金莲 韦宗堂 《新疆医科大学学报》 CAS 2001年第2期182-182,共1页
关键词 儿童 碱性碱酸酶 血清诊断 性别 民族
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慢生大豆根瘤菌存在碱性磷酸酶的报告
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作者 唐欣昀 钱宪强 《微生物学杂志》 CAS CSCD 1989年第2期14-18,29,共6页
利用磷饥饿的方法,在一株慢生大豆根瘤菌中发现可作为胞周蛋白标记酶的高活性碱性磷酸酶。该酶属诱导型,当磷酸盐浓度降至55μM时诱导已相当充分。培养物上清液中没有酶活。使用溶菌酶——EDTA和冷渗冲击的方法均没有提取出该酶。推测... 利用磷饥饿的方法,在一株慢生大豆根瘤菌中发现可作为胞周蛋白标记酶的高活性碱性磷酸酶。该酶属诱导型,当磷酸盐浓度降至55μM时诱导已相当充分。培养物上清液中没有酶活。使用溶菌酶——EDTA和冷渗冲击的方法均没有提取出该酶。推测该酶可能与胞周空间外侧、外膜内侧的脂多糖结合。 展开更多
关键词 慢生大豆根瘤菌 胞周空间 碱性碱酸酶 溶菌
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Use of a Bioaugmented Organic Soil Amendment in Combination with Gypsum for Withania somnifera Growth on Sodic Soil 被引量:7
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作者 Manjul GUPTA Pankaj Kumar SRIVASTAVA +2 位作者 SHIKHA Abhishek NIRANJAN Shri Krishna TEWARI 《Pedosphere》 SCIE CAS CSCD 2016年第3期299-309,共11页
Limited availability of organic matter is a problem to sustain crop growth on sodic soil. Organic soil amendments are a costeffective source of nutrients to enhance crop growth. A field study was conducted to evaluate... Limited availability of organic matter is a problem to sustain crop growth on sodic soil. Organic soil amendments are a costeffective source of nutrients to enhance crop growth. A field study was conducted to evaluate the effect of an organic soil amendment bioaugmented with plant growth-promoting fungi(SF_(OA) ) in combination with gypsum on soil properties and growth and yield attributes of Withania somnifera, one of the most valuable crops of the traditional medicinal system in the world, on a sodic soil at the Aurawan Research Farm of CSIR-National Botanical Research Institute, Lucknow, India. The SF_(OA) used was prepared by pre-enriching farm waste vermicompost with plant growth-promoting fungi before mixing with pressmud and Azadirachta indica seed cake. The application of SF_(OA) at 10 Mg ha^(-1)after gypsum(25.0 Mg ha^(-1)) treatment significantly(P < 0.05) increased root length(by 96%) and biomass(by 125%) of Withania plants compared to the control without SF_(OA) and gypsum. Similarly, the highest withanolide contents were observed in leaves and roots of Withania plants under 10 Mg ha^(-1)SF_(OA) and gypsum. Combined application of SF_(OA) and gypsum also improved physical, chemical and enzymatic properties of the soil, with the soil bulk density decreasing by 25%, water-holding capacity increasing by 121%, total organic C increasing by 90%, p H decreasing by 17% and alkaline phosphatase, β-glucosidase, dehydrogenase and cellulase activities increasing by 54%, 128%, 81% and 96%, respectively, compared to those of the control. These showed that application of the SF_(OA) tested in this study might reclaim sodic soil and further support Withania cultivation and results were better when the SF_(OA) was applied after gypsum treatment. 展开更多
关键词 medicinal crop plant growth-promoting fungi soil property soil reclamation vermicompost
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