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肝炎患者血清碱性谷胱甘肽S转移酶的放射免疫测定
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作者 王小红 李梦东 《中华医学杂志》 CAS CSCD 北大核心 1990年第8期459-460,共2页
关键词 病毒性肝炎 碱性谷胱甘肽 转移酶 放射免疫测定
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人碱性谷胱甘肽S—转移酶的提纯,抗血清制备及初步临床应用
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作者 伍严安 王钧熔 《中华医学检验杂志》 CSCD 1989年第6期342-345,共4页
关键词 碱性谷胱甘肽 转移酶 抗血清 制备 病毒性肝炎
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融合蛋白GSH-bFGF的构建、表达及体外活性检测 被引量:1
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作者 陈霖 周天鸿 邹奕 《暨南大学学报(自然科学与医学版)》 CAS CSCD 北大核心 2015年第2期108-113,共6页
目的:设计构建和表达新型融合蛋白谷胱甘肽-碱性成纤维细胞生长因子(Glutathione-basic fibroblast growth factor,GSH-b FGF),并检测其体外抗氧化、抗衰老能力.方法:采用RT-PCR,酶切,连接,转化等分子克隆技术构建新型融合蛋白GSH-b FGF... 目的:设计构建和表达新型融合蛋白谷胱甘肽-碱性成纤维细胞生长因子(Glutathione-basic fibroblast growth factor,GSH-b FGF),并检测其体外抗氧化、抗衰老能力.方法:采用RT-PCR,酶切,连接,转化等分子克隆技术构建新型融合蛋白GSH-b FGF,并通过镍柱亲和层析、阳离子交换层析纯化该目的蛋白.通过CCK-8试剂盒检测GSH-b FGF对鼠源成纤维细胞NIH-3T3的促增殖活性.同时,经总抗氧化检测试剂盒检测GSH-b FGF的抗氧化能力.结果:克隆表达并纯化获得融合蛋白GSH-b FGF.CCK-8促增殖结果显示质量浓度为20 ng/m L的GSH-b FGF可显著促进鼠源成纤维细胞NIH-3T3增殖.体外抗氧化结果显示GSH-b FGF有显著的抗氧化活性,纯化得到的GSHb FGF蛋白的GSH浓度约为(0.06±0.02)μmol/L,抗氧化能力为(0.56±0.23)mmol/g.结论:新型融合蛋白GSHb FGF有显著的体外促增殖能力及抗氧化活性. 展开更多
关键词 -碱性成纤维生长因子 促增殖能力 抗氧化活性
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Variation in Salt Tolerance of Wheat Cultivars:Role of Glycinebetaine and Ethylene 被引量:5
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作者 M.I.R.KHAN N.IQBAL +1 位作者 A.MASOOD N.A.KHAN 《Pedosphere》 SCIE CAS CSCD 2012年第6期746-754,共9页
Four wheat (Triticum aestivum L.) cultivars 711, PBW343, 3765 and WH542 were screened for studying variations in glycinebetaine (GB) content and plant dry mass under 100 mmol L-1 NaCl stress. A tolerance index was... Four wheat (Triticum aestivum L.) cultivars 711, PBW343, 3765 and WH542 were screened for studying variations in glycinebetaine (GB) content and plant dry mass under 100 mmol L-1 NaCl stress. A tolerance index was calculated using plant dry mass data to select salt-tolerant and salt-sensitive types and find association between tolerance index and GB content. Tolerance index has been used as a good criterion to select the tolerant types under high salinity stress. Further, physiological differences in salt-tolerant cultivar 711 and salt-sensitive cultivar WH542 were examined. The salt-tolerant cultivar exhibited greater CB content, which was found correlative with ethylene. The cultivar also showed higher nitrogen (N) content and nitrate reductase activity, reduced glutathione and higher redox state resulting in maximal protection of plant dry mass than the salt-sensitive type. Thus, the content of CB may be considered as important physiological criteria for selecting salt-tolerant wheat types. 展开更多
关键词 glutathione nitrogen assimilation salt stress
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Protective effect of Sharbat-e-Deenar against acetaminophen-induced hepatotoxicity in experimental animals
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作者 Arvind Kumar Shakya Sangeeta Shukla 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2017年第3期387-392,共6页
OBJECTIVE: To investigate the effect of Sharbat-e-Deenar(SD) on acetaminophen(APAP)-induced hepatotoxicity in rat model.METHODS: Albino rats were treated with SD at the doses of 1, 2 and 4 mL/kg, p.o. against hepatoto... OBJECTIVE: To investigate the effect of Sharbat-e-Deenar(SD) on acetaminophen(APAP)-induced hepatotoxicity in rat model.METHODS: Albino rats were treated with SD at the doses of 1, 2 and 4 mL/kg, p.o. against hepatotoxicity after APAP(2 g/kg, p.o. once only) intoxication.The blood, tissue biochemical parameters and histopathological observation were performed. The RESULTS: APAP exposure in rats significantly increased the level of biochemical parameters such as aspartate aminotransaminase, alanine aminotransaminase, lactate dehydrogenase, serum alkaline phosphatase, bilirubin, urea and creatinine into blood circulation which were reversed towards normal by SD therapy at all doses. The tissue biochemical parameters such as lipid peroxidation, reduced glutathione, adenosine tri-phosphatase and glucose-6-phosphatase were significantly restored after SD treatment against hepatotoxity. Histological analysis confirmed that SD-treated rats significantly alleviated of liver damage and reduced lesions caused by APAP intoxication. The biochemical changes are in good correlation with the histopathological observations.CONCLUSION: On the basis of these results, it can be concluded that SD exerts hepatoprotective activity against APAP-induced liver injury. 展开更多
关键词 Sharbat-e-Deenar ACETAMINOPHEN Hepatitis toxic HEPATOPROTECTION
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