玉米麸皮不同处理及其成分分析

为探讨玉米麸皮的生理功能,需对玉米麸皮进行处理。通过酶处理除去玉米麸皮中含量较多的蛋白质和淀粉,得到精制玉米麸皮并确定最佳酶解工艺。再分别对精制玉米麸皮进行酸碱处理,得到相应的酸碱解产物。对玉米麸皮、精制玉米麸皮、酸解产物和碱解产物基本成分组成及膳食纤维组成进行分析。结果显示碱性蛋白酶去除玉米麸皮蛋白质的作用要好于酸性蛋白酶和中性蛋白酶。蛋白酶和淀粉酶能使玉米麸皮中的蛋白质和淀粉由10.32%和17.5%分别减少到1.65%和1.23%。精制玉米麸皮总膳食纤维比玉米麸皮增加26%。酸解产物和碱解产物基本成分是可溶性膳食纤维,其中可溶性半纤维素分别为83.52%和86.82%,不含纤维素和木质素。

不同处理的精制玉米麸皮单糖组分及阿魏酸含量分析

对去除淀粉和蛋白质的精制玉米麸皮进行酸碱处理,得到酸解产物和碱解产物,用高效液相色谱对精制玉米麸皮、酸解产物及碱解产物的单糖组成及阿魏酸进行分析。结果显示:酸解产物含有44.5%的木糖、20.8%的阿拉伯糖和4.57%的阿魏酸,推测其主要成分是阿魏酸阿拉伯木聚糖;碱解产物含有50.3%的木糖、24.5%的阿拉伯糖,阿魏酸基本未检出,推测其主要成分是阿拉伯木聚糖;玉米麸皮含有3.5%的阿魏酸。

Isolation of Mimosine Degrading Bacteria from Rumen Juice and Mass Production by Gottingen Bioreactor Technology

A method is described to isolate mfinosine degrading bacteria from mmen juice from German steers which had never been ted on either leucaena or mimosine. The isolate was cultivated in continuous culture in a bench top fermenter in Medium 98-5 amended with an increasing amount of mimosine for 16 days. Degradation of mimosine was determined with a colorrinetfic method Incubated at 39 ℃ under anaerobic conditions, the isolate showed complete degradation of mimosine within one ~veek. The bacteriological pure isolate is an aero-tolerant gram-negative cocco-bacillus. By DNA sequencing analysis it belongs to genus Klebsiella. It was multiplied hi brain-heart-infusion, entrapped in alginate, and dried （37 ℃）. Feeding trials in sheep in Myalmlar showed complete innocuity＇ and complete detoxification of mimosine from leucaena leaves.