Carbonic anhydrases(CA)is a zinc-enzyme family。They are involved in multiple aspects of systemic and cellular acid-base balance.This review is a general introduction to the disposition,molecular constitution,biologic...Carbonic anhydrases(CA)is a zinc-enzyme family。They are involved in multiple aspects of systemic and cellular acid-base balance.This review is a general introduction to the disposition,molecular constitution,biological function and physiological function of CA.展开更多
In order to learn the ways and possible utilization mechanisms of dissolved inorganic carbon (DIC) in marine phytoplankton species under carbon-replete or -limited conditions, the activity of extracellular carbonic an...In order to learn the ways and possible utilization mechanisms of dissolved inorganic carbon (DIC) in marine phytoplankton species under carbon-replete or -limited conditions, the activity of extracellular carbonic anhydrase (CA) was assayed in different pH, CO 2 and DIC concentrations. Extracellular CA in Amphidinium carterae and Prorocentrum minimum was detected under carbon-replete conditions, while in Melosira sp., Phaeodactylum tricornutum, Skeletonema costatum, Thalassiosira rotula, Emiliania huxleyi and Pleurochrysis carterae, CA activity was assayed under conditions of carbon limitation. No CA activity was found even under carbon-limited conditions in Chaetoceros compressus, Glenodinium foliaceum, Coccolithus pelagicus, Gephrocapsa oceanica and Heterosigma akashiwo. In species without extracellular CA activity, the direct HCO - 3 uptake was investigated using a pH drift technique and the anion exchange inhibitor 4′4′-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS) in a closed system. The result showed that direct HCO - 3 transport might occur by an anion exchange mechanism in species Coc. pelagicus and G. oceanica. Of the 13 species investigated, only H. akashiwo did not have the potential for direct uptake or extracellular CA-catalyzed HCO - 3 utilization.展开更多
Thirty-four sampling sites for surface water and twenty-seven sampling sites for ground water in areas of different land use were selected in the Wujiang River Basin, upstream section in Guizhou Province, China. Carbo...Thirty-four sampling sites for surface water and twenty-seven sampling sites for ground water in areas of different land use were selected in the Wujiang River Basin, upstream section in Guizhou Province, China. Carbonic anhydrase （CA） activity in surface and ground water was analyzed and compared. The results show that CA activity was detected in all surface and ground water samples. In general, average CA activity in surface and ground water was highest from samples in arbor lands, followed by shrub lands, shrub and grass lands, agricultural lands, and residential areas. The average CA activity in ground water from arbor lands, shrub lands, and shrub and grass lands was higher than that in surface water. Water CA activity was positively correlated with HCO3- concentration in surface water and ground water. These results imply that the contribution of CA in water to the karst carbon sink potential of river basins warrants further extensive and in-depth research.展开更多
文摘Carbonic anhydrases(CA)is a zinc-enzyme family。They are involved in multiple aspects of systemic and cellular acid-base balance.This review is a general introduction to the disposition,molecular constitution,biological function and physiological function of CA.
文摘In order to learn the ways and possible utilization mechanisms of dissolved inorganic carbon (DIC) in marine phytoplankton species under carbon-replete or -limited conditions, the activity of extracellular carbonic anhydrase (CA) was assayed in different pH, CO 2 and DIC concentrations. Extracellular CA in Amphidinium carterae and Prorocentrum minimum was detected under carbon-replete conditions, while in Melosira sp., Phaeodactylum tricornutum, Skeletonema costatum, Thalassiosira rotula, Emiliania huxleyi and Pleurochrysis carterae, CA activity was assayed under conditions of carbon limitation. No CA activity was found even under carbon-limited conditions in Chaetoceros compressus, Glenodinium foliaceum, Coccolithus pelagicus, Gephrocapsa oceanica and Heterosigma akashiwo. In species without extracellular CA activity, the direct HCO - 3 uptake was investigated using a pH drift technique and the anion exchange inhibitor 4′4′-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS) in a closed system. The result showed that direct HCO - 3 transport might occur by an anion exchange mechanism in species Coc. pelagicus and G. oceanica. Of the 13 species investigated, only H. akashiwo did not have the potential for direct uptake or extracellular CA-catalyzed HCO - 3 utilization.
基金Sub-project of Geological Survey Work Item of China Geological Survey Bureau(Grant No.12120113005200)the Fundamental Research Funds for the Central Universities(Grant No.2015QN102)
文摘Thirty-four sampling sites for surface water and twenty-seven sampling sites for ground water in areas of different land use were selected in the Wujiang River Basin, upstream section in Guizhou Province, China. Carbonic anhydrase （CA） activity in surface and ground water was analyzed and compared. The results show that CA activity was detected in all surface and ground water samples. In general, average CA activity in surface and ground water was highest from samples in arbor lands, followed by shrub lands, shrub and grass lands, agricultural lands, and residential areas. The average CA activity in ground water from arbor lands, shrub lands, and shrub and grass lands was higher than that in surface water. Water CA activity was positively correlated with HCO3- concentration in surface water and ground water. These results imply that the contribution of CA in water to the karst carbon sink potential of river basins warrants further extensive and in-depth research.
