Biomedical applications of porous calcium car- bonate （CaCO3） microspheres have been mainly restricted by their aqueous instability and low remineralization rate. To overcome these obstacles, a novel symmetry-breaki...Biomedical applications of porous calcium car- bonate （CaCO3） microspheres have been mainly restricted by their aqueous instability and low remineralization rate. To overcome these obstacles, a novel symmetry-breaking assembled porous calcite microsphere （PCMS） was con- structed in an ethanol/water mixed system using a two-step vapor-diffusion/aging crystallization strategy. In contrast to the conventional additive-induced crystallization method, the present strategy was performed under mild conditions and was free from any foreign additives, thus avoiding the potential contamination of the final product. Meanwhile, the prepared PCMSs were characterized by their highly uniform spherical morphology and large open pores, which are fa- vorable for large protein delivery. An antimicrobial study of immunoglobulin Y （IgY）-loaded PCMSs revealed excellent antimicrobial activity against Streptococcus mutans. More importantly, they showed surprisingly rapid transformation to bone minerals in physiological medium. Evaluation of the in vitro efficacy of PCMSs in dentinal tubule occlusion demonstrated their powerful potential to serve as a catalyst in the repair of dental hard tissue. Therefore, the developed PCMSs show great promise as multifunctional biomaterials for dental treatment applications.展开更多
文摘利用墨鱼骨纹层状基质和背盾粉末为原料,利用水热法在120,150,180℃不同温度下反应8 h/24 h制备羟基磷灰石,分别利用SEM,XRD等方法分析墨鱼骨纹层状基质和背盾,以及对应的反应产物.研究表明:墨鱼骨纹层状基质和背盾的主要矿物成分为文石相碳酸钙,以及少量方解石相碳酸钙.以纹层状基质粉末为原料制备羟基磷灰石的最佳水热反应条件为:温度120℃、反应时间8 h,以背盾粉末为原料制备羟基磷灰石的最佳水热反应条件为:温度180℃、反应时间8 h.
基金supported by the National Natural Science Foundation ofChina (51402329 and 81500806)the Science Foundation for Youth Scholar of State Key Laboratory of High Performance Ceramics and Superfine Microstructures (SKL201404)Shanghai Excellent Academic Leaders Program (14XD1403800)
文摘Biomedical applications of porous calcium car- bonate （CaCO3） microspheres have been mainly restricted by their aqueous instability and low remineralization rate. To overcome these obstacles, a novel symmetry-breaking assembled porous calcite microsphere （PCMS） was con- structed in an ethanol/water mixed system using a two-step vapor-diffusion/aging crystallization strategy. In contrast to the conventional additive-induced crystallization method, the present strategy was performed under mild conditions and was free from any foreign additives, thus avoiding the potential contamination of the final product. Meanwhile, the prepared PCMSs were characterized by their highly uniform spherical morphology and large open pores, which are fa- vorable for large protein delivery. An antimicrobial study of immunoglobulin Y （IgY）-loaded PCMSs revealed excellent antimicrobial activity against Streptococcus mutans. More importantly, they showed surprisingly rapid transformation to bone minerals in physiological medium. Evaluation of the in vitro efficacy of PCMSs in dentinal tubule occlusion demonstrated their powerful potential to serve as a catalyst in the repair of dental hard tissue. Therefore, the developed PCMSs show great promise as multifunctional biomaterials for dental treatment applications.
