Objective： To fabricate polymeric nanocomposites with excellent photoluminescence, magnetic properties, and stability in aqueous solutions, in order to improve specificity and sensitivity of cellular imaging under a ...Objective： To fabricate polymeric nanocomposites with excellent photoluminescence, magnetic properties, and stability in aqueous solutions, in order to improve specificity and sensitivity of cellular imaging under a magnetic field. Methods： Fluoridated LnS＋-doped HAP （Ln3＋-HAP） NPs and iron oxides （lOs） can be encapsulated with biocompatible polymers via a modified solvent exaction/evaporation technique to prepare polymeric nanocomposites with fluoridated Ln3＋-HAP/iron oxide. The nanocomposites were characterized for surface morphology, fluorescence spectra, magnetic properties and in vitro cytotoxicity. Magnetic targeted cellular imaging of such nanocomposites was also evaluated with confocal laser scanning microscope using A549 cells with or without magnetic field. Results： The fabricated nanocomposites showed good stability and excellent luminescent properties, as well as low in vitro cytotoxicity, indicating that the nanocomposites are suitable for biological applications. Nanocomposites under magnetic field achieved much higher cellular uptake via an energy-dependent pathway than those without magnetic field. Conclusion： 1tie nanocomposites fabricated in this study will be a promising tool for magnetic targeted cellular imaging with improved specificity and enhanced selection.展开更多
Smart nanoparticles that respond to pathophysiological parameters,such as p H,GSH,and H2O2,have been developed with the huge and urgent demand for the high-efficient drug delivery systems(DDS)for cancer therapy.Herein...Smart nanoparticles that respond to pathophysiological parameters,such as p H,GSH,and H2O2,have been developed with the huge and urgent demand for the high-efficient drug delivery systems(DDS)for cancer therapy.Herein,cubic poly(ethylene glycol)(PEG)-modified mesoporous amorphous iron oxide(AFe)nanoparticles(AFe-PEG)have been successfully prepared as p H-stimulated drug carriers,which can combine doxorubicin(DOX)with a high loading capacity of 948 mg/g,forming a novel multifunctional AFe-PEG/DOX nanoparticulate DDS.In an acidic microenvironment,the AFe-PEG/DOX nanoparticles will not only release DOX efficiently,but also release Fe ions to catalyze the transformation of H2O2 to·OH,acting as fenton reagents.In vitro experimental results proved that the AFe-PEG/DOX nanoparticles can achieve combination of chemotherapeutic(CTT)and chemodynamic therapeutic(CDT)effects on Hela tumor cells.Furthermore,the intrinsic magnetism of AFePEG/DOX makes its cellular internalization efficiency be improved under an external magnetic field.Therefore,this work develops a new and promising magnetically targeted delivery and dual CTT/CDT therapeutic nano-medicine platform based on amorphous iron oxide.展开更多
基金supported by National Natural Science Foundation of China (Grant No. 21506161, 31270019)National Key Basic Research Program of China (973 Program) (Grant No. 2011CB933100, 2011CB932402)+1 种基金Guangdong Natural Science Funds for Distinguished Young Scholar (Grant No. 2014A030306036)open funds from the Key Laboratory of Biomedical Materials in Tianjin
文摘Objective： To fabricate polymeric nanocomposites with excellent photoluminescence, magnetic properties, and stability in aqueous solutions, in order to improve specificity and sensitivity of cellular imaging under a magnetic field. Methods： Fluoridated LnS＋-doped HAP （Ln3＋-HAP） NPs and iron oxides （lOs） can be encapsulated with biocompatible polymers via a modified solvent exaction/evaporation technique to prepare polymeric nanocomposites with fluoridated Ln3＋-HAP/iron oxide. The nanocomposites were characterized for surface morphology, fluorescence spectra, magnetic properties and in vitro cytotoxicity. Magnetic targeted cellular imaging of such nanocomposites was also evaluated with confocal laser scanning microscope using A549 cells with or without magnetic field. Results： The fabricated nanocomposites showed good stability and excellent luminescent properties, as well as low in vitro cytotoxicity, indicating that the nanocomposites are suitable for biological applications. Nanocomposites under magnetic field achieved much higher cellular uptake via an energy-dependent pathway than those without magnetic field. Conclusion： 1tie nanocomposites fabricated in this study will be a promising tool for magnetic targeted cellular imaging with improved specificity and enhanced selection.
基金supported by the National Natural Science Foundation of China(No.51473152 and No.51573174)Scientific Research Foundation for Young Talents from Fujian Provincial Department of Education(No.JT180494)Scientific Research Platform Construction Project from Fujian Provincial Department of Science and Technology(No.2018H2002)。
文摘Smart nanoparticles that respond to pathophysiological parameters,such as p H,GSH,and H2O2,have been developed with the huge and urgent demand for the high-efficient drug delivery systems(DDS)for cancer therapy.Herein,cubic poly(ethylene glycol)(PEG)-modified mesoporous amorphous iron oxide(AFe)nanoparticles(AFe-PEG)have been successfully prepared as p H-stimulated drug carriers,which can combine doxorubicin(DOX)with a high loading capacity of 948 mg/g,forming a novel multifunctional AFe-PEG/DOX nanoparticulate DDS.In an acidic microenvironment,the AFe-PEG/DOX nanoparticles will not only release DOX efficiently,but also release Fe ions to catalyze the transformation of H2O2 to·OH,acting as fenton reagents.In vitro experimental results proved that the AFe-PEG/DOX nanoparticles can achieve combination of chemotherapeutic(CTT)and chemodynamic therapeutic(CDT)effects on Hela tumor cells.Furthermore,the intrinsic magnetism of AFePEG/DOX makes its cellular internalization efficiency be improved under an external magnetic field.Therefore,this work develops a new and promising magnetically targeted delivery and dual CTT/CDT therapeutic nano-medicine platform based on amorphous iron oxide.
