Testing methods of instrumental system in the marine magnetic survey have been studied in this paper, and the feasibility of each method has been testified by the observed data. The conclusion shows that the method br...Testing methods of instrumental system in the marine magnetic survey have been studied in this paper, and the feasibility of each method has been testified by the observed data. The conclusion shows that the method brought out can effectively eliminate the systematic error caused by the instrumental system, and greatly improve the surveying precision and the reliability of the survey results.展开更多
Objective To investigate the feasibility of magnetic resonance （MR） diffusion weighted imaging （DWI） in discriminating inflammatory from VX2 carcinoma metastatic lymph nodes in rabbit model. Methods Twenty New Ze...Objective To investigate the feasibility of magnetic resonance （MR） diffusion weighted imaging （DWI） in discriminating inflammatory from VX2 carcinoma metastatic lymph nodes in rabbit model. Methods Twenty New Zealand white rabbits were randomly divided into 2 groups. Complete Freund＇s adjuvant was injected into the bilateral dorsal footpads to set up ipsilateral lymphadenitis model （n = 10）, and the other 10 rabbits received a subcutaneous implantation of VX2 tumor cell suspension （1.5×10^7 cells/mL） in both thighs to set up metastatic lymph node model. MR imaging scan covering the popliteal fossa and lilac fossa including short time inversion recovery echo-planar imaging DWI （STIR-EPI-DWI）, Tl-weighted imaging （T1WI） and T2-weighted imaging （T2WI） was performed 2 weeks after injection. T2WI signal intensity （SI）, DWI SI, long/short axial ratio （LSR） and apparent diffusion coefficient （ADC） values of the lymph nodes were evaluated in all cases. Right after MR imaging scan, popliteal and iliac fossa lymph nodes were collected for hematoxylin-eosin staining. Results Totally 33 lymph nodes larger than 5 turn, including 22 inflammatory and 11 metastatic ones, were successfully isolated and taken into pathological analysis. LSR showed no significant difference between the inflammatory and malignant lymph nodes （P 〉 0.05）. Both benign and malignant lymph nodes appeared iso-intense on TlWI and hyperintense on both T2WI and DWI images with an even lower TlWI and higher T2WI SI core at the hilum. Both T2WI and DWI SI showed no significant difference between two pathological groups （P 〉 0.01） in popliteal fossa. The mean ADC value of inflammatory nodes [（1.199±0.281） ×10^-3 mm^2/s] was significantly higher than that of metastatic nodes [（0.858 ± 0.090） ×10^-3 mm^2/s, P 〈 0.01]. On ADC map, a high ADC value central area could be seen in most of the lymph nodes no matter benign or malignant. ADC value gave out the largest area under curve （AUCADC = 0.955） compared with other three indexes （AUCLsR = 0.488; AUCT2WI SI = 0.727; AUCDDWISI = 0.822） and gave the best sensitivity and specificity in lymph node differential diagnosis compared with the other three indexes. Conclusions High quality DWI image can be obtained using STIR-EPI-DWI sequence in rabbit model. DWI is a new promising technique for differentiating inflammatory from metastatic lymph nodes. Compared with routine MR sequence, DWI could provide more useful physiological and functional information for diagnosis.展开更多
Particle Impact Noise Detection (PIND) test is a reliability screening technique for hermetic device that is prescribed by MIL-PRF-39016E. Some test conditions are specified, although MIL-PRF-39016E did not specify ho...Particle Impact Noise Detection (PIND) test is a reliability screening technique for hermetic device that is prescribed by MIL-PRF-39016E. Some test conditions are specified, although MIL-PRF-39016E did not specify how to obtain these condi- tions. This paper establishes the dynamics model of vibration process based on first order mass-spring system. The corresponding Simulink model is also established to simulate vibration process in optional input excitations. The response equations are derived in sinusoidal excitations and the required electromagnetic force waves are computed in order to obtain a given vibration and shock accelerations. Last, some simulation results are given.展开更多
AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these paper.METHODS:The principle of the assay is the imm...AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these paper.METHODS:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 antibody.The detection signal was acquired from the fluorescence signal of QDs.For the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line(DLD-1) and CTCs from CRC patients' peripheral blood.RESULTS:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer.Fluorescenceactivated cell sorting analysis and Real Time RT-PCR,they both have also been used to evaluate the performance of the described method.In conclusion,we developed a simple,sensitive,efficient and of lower cost(than the existing ones) method for the detection of CRC CTCs in human samples.We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.CONCLUSION:The method described here can be easily adjusted for any other protein target of either the CTC or the host.展开更多
文摘Testing methods of instrumental system in the marine magnetic survey have been studied in this paper, and the feasibility of each method has been testified by the observed data. The conclusion shows that the method brought out can effectively eliminate the systematic error caused by the instrumental system, and greatly improve the surveying precision and the reliability of the survey results.
文摘Objective To investigate the feasibility of magnetic resonance （MR） diffusion weighted imaging （DWI） in discriminating inflammatory from VX2 carcinoma metastatic lymph nodes in rabbit model. Methods Twenty New Zealand white rabbits were randomly divided into 2 groups. Complete Freund＇s adjuvant was injected into the bilateral dorsal footpads to set up ipsilateral lymphadenitis model （n = 10）, and the other 10 rabbits received a subcutaneous implantation of VX2 tumor cell suspension （1.5×10^7 cells/mL） in both thighs to set up metastatic lymph node model. MR imaging scan covering the popliteal fossa and lilac fossa including short time inversion recovery echo-planar imaging DWI （STIR-EPI-DWI）, Tl-weighted imaging （T1WI） and T2-weighted imaging （T2WI） was performed 2 weeks after injection. T2WI signal intensity （SI）, DWI SI, long/short axial ratio （LSR） and apparent diffusion coefficient （ADC） values of the lymph nodes were evaluated in all cases. Right after MR imaging scan, popliteal and iliac fossa lymph nodes were collected for hematoxylin-eosin staining. Results Totally 33 lymph nodes larger than 5 turn, including 22 inflammatory and 11 metastatic ones, were successfully isolated and taken into pathological analysis. LSR showed no significant difference between the inflammatory and malignant lymph nodes （P 〉 0.05）. Both benign and malignant lymph nodes appeared iso-intense on TlWI and hyperintense on both T2WI and DWI images with an even lower TlWI and higher T2WI SI core at the hilum. Both T2WI and DWI SI showed no significant difference between two pathological groups （P 〉 0.01） in popliteal fossa. The mean ADC value of inflammatory nodes [（1.199±0.281） ×10^-3 mm^2/s] was significantly higher than that of metastatic nodes [（0.858 ± 0.090） ×10^-3 mm^2/s, P 〈 0.01]. On ADC map, a high ADC value central area could be seen in most of the lymph nodes no matter benign or malignant. ADC value gave out the largest area under curve （AUCADC = 0.955） compared with other three indexes （AUCLsR = 0.488; AUCT2WI SI = 0.727; AUCDDWISI = 0.822） and gave the best sensitivity and specificity in lymph node differential diagnosis compared with the other three indexes. Conclusions High quality DWI image can be obtained using STIR-EPI-DWI sequence in rabbit model. DWI is a new promising technique for differentiating inflammatory from metastatic lymph nodes. Compared with routine MR sequence, DWI could provide more useful physiological and functional information for diagnosis.
文摘Particle Impact Noise Detection (PIND) test is a reliability screening technique for hermetic device that is prescribed by MIL-PRF-39016E. Some test conditions are specified, although MIL-PRF-39016E did not specify how to obtain these condi- tions. This paper establishes the dynamics model of vibration process based on first order mass-spring system. The corresponding Simulink model is also established to simulate vibration process in optional input excitations. The response equations are derived in sinusoidal excitations and the required electromagnetic force waves are computed in order to obtain a given vibration and shock accelerations. Last, some simulation results are given.
基金Supported by The John S Latsis Public Benefit FoundationThe Hellenic Society of Medical Oncology
文摘AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these paper.METHODS:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 antibody.The detection signal was acquired from the fluorescence signal of QDs.For the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line(DLD-1) and CTCs from CRC patients' peripheral blood.RESULTS:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer.Fluorescenceactivated cell sorting analysis and Real Time RT-PCR,they both have also been used to evaluate the performance of the described method.In conclusion,we developed a simple,sensitive,efficient and of lower cost(than the existing ones) method for the detection of CRC CTCs in human samples.We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.CONCLUSION:The method described here can be easily adjusted for any other protein target of either the CTC or the host.
