磁疗技术治疗Ⅱ度内痔的临床疗效初步探讨

目的探讨磁疗技术治疗Ⅱ度内痔的临床疗效。方法纳入2019年8月至2020年2月福建医科大学附属协和医院肛肠科门诊就诊的90例Ⅱ度内痔患者为研究对象,采用随机数字表法分为治疗组(采用磁疗棒治疗,n=45)与对照组(采用聚桂醇注射治疗,n=45)。治疗组接受以10天为1个疗程、共2个疗程的治疗。比较两组治疗结束20天的临床疗效,以及两组治疗前、治疗结束10天、治疗结束20天的肛管直肠压力指标(肛管静息压、肛管最大收缩压)及症状积分(主症状积分包括便血、脱垂、痔核黏膜情况,次症状积分包括肛门坠胀、肛周潮湿情况)。结果治疗组与对照组治疗总有效率分别为95.6%、100%,组间比较差异无统计学意义(P>0.05)。治疗组肛管静息压、主症状积分、次症状积分均低于对照组(均P<0.05);前述三个指标水平随测量时间延长均呈逐渐降低趋势(均P<0.05)。两组肛管最大收缩压比较差异无统计学意义(P>0.05);肛管最大收缩压未随测量时间延长有所改变(P>0.05)。结论应用磁疗技术治疗Ⅱ度内痔可取得与聚桂醇注射疗法相近的短期疗效,且在减轻患者临床症状方面表现出一定的应用优势。

磁场对家兔血清谷胱甘肽过氧化物酶及血液过氧化氢酶活性的影响

目的 :探讨磁场对家兔谷胱甘肽过氧化物酶 ( GSH- Px)和过氧化氢酶 ( CAT)活性的影响。方法 :将 2 0只家兔随机分为实验组与对照组。实验组每天进行一次磁处理 ,每次 30 min。在家兔腹背贴磁片 ,每一磁片的磁感应强度 0 .2 T。6周后测定两组家兔血清中 GSH- Px和血液中 CAT的活性。结果 :实验组 GSH- Px活性为 ( 1 438.5 0± 1 5 7.89)活力单位 /ml,CAT活性为 ( 68.2 9± 1 3.91 )活力单位 /L。对照组 GSH- Px活性为 ( 1 1 87.73± 2 72 .5 5 )活力单位 /ml,CAT活性为 ( 5 5 .69± 7.5 7)活力单位 /L。实验组 GSH- Px与 CAT活性明显升高 ( P<0 .0 5 )。结论 :磁场能够增强 GSH- Px和 CAT的活性 ,提高机体处理自由基的能力 ,增加机体的抵抗能力 ,具有一定的抗衰老功能。

Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its molecular mechanism

Objective： To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer. Methods： Plasmids pcDNA3-hLRH-1 were introduced into SW480 cells via lipofectamine. The expression of mRNA and protein of exogenous hLRH-1 were detected by RT-PCR and western blotting, respectively. MTT assay was carried out to survey the proliferation of SW480 cells with overexpression of hLRH-1. Meanwhile, the expression of proliferation-related genes cyclin E1 and cyclin D1, and apoptosis-related genes PTEN and Rbl, were analyzed by realtime RT-PCR. Results： The proliferation of SW480 cells was promoted under the condition of overexpression of hLRH-1. The expression of cyclin E1 was up-regulated significantly, while that of PTEN and Rbl were down-regulated in SW480 cells with overexpressed hLRH-1. Conclusion： The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1, as well as participated in the regulation of apoptosis via influencing the expression of PTEN and Rb1.

EVALUATION OF NEUROPROTECTIVE EFFECTS OF LONG-TERM LOW DOSE HORMONE REPLACEMENT THERAPY ON POSTMENOPAUSAL WO-MEN BRAIN HIPPOCAMPUS USING MAGNETIC RESONANCE SCANNER

To investigate the effects of long-term low dose hormone replacement therapy （HRT） on postmenopaosal women in homone level, cognition score, hippocampus volume, and magnetic resonance spectroscopy （MRS） parameters. Methods A total of 182 postmenopausal women aged 50-87 years were chosen at Peking Union Medical College Hospital and assigned to HRT group and control group. The volunteers of HRT group had taken low dose hormone [ estradiol （E2 ） 0. 5-1.0 mg and progesterone 0.5-2.0 mg, once a day ] for 4-33 years. The concentrations of E2, progesterone, and testosterone were measured using enzyme-linked immunosorbent assay （ELISA）. The gene types of apolipoprotein E （ApoE） were measured by polymerase chain reaction, and the subjects with susceptible genes （ ApoE ε3/ε4） of Alzheimer＇s disease （AD） were screened. Their hippocampus volumes and MRS parameters were obtained through magnetic resonance imaging （MRI）, and results in two groups were analyzed by statistical method. Results Compared with control group, the concentrations of E2 at each age stage in HRT group were significantly higher （P 〈0. 05） except the 80-89 years old subgroup; yet, there were no statistical differences in the concentrations of progesterone and testosterone between the two groups. There was no obvious difference in ApoE subtypes distribution between the two groups The results of hippocampus MRI for the subjects with susceptible genes ApoE ε3/ε4 （HRT group 14 cases, control group 11 cases） showed that the ratio of bilateral hippocampus volume to whole brain volume in HRT group （0. 406 ± 0.028） was signiticantlyhigher than control gronp （0.369±0.031, P〈0.05）. Theresults of ^1H MRS for the subjects with susceptible genes ApoE ε3/ε4 （ HRT group 12 cases, control group 11 cases） showed that the N-acetylaspartate/total creatine at the area of hippocampus in HRT group （ 1.54±0. 08 ） were significantly higher than control group （ 1.45±0. 13, P 〈 0. 05）. Conclusions For postmenopausal women, long-term low dose HRT can maintain the physiological concentration of E2 in plasma. Furthermore, the hippocampus MRI performed on those with ApoE ε3/ε3 genes shows that long-term low dose HRT can prevent hippocampus atrophy, which is beneficial to maintain the brain function and prevent AD.

Image formation of brain function in patients suffering from knee osteoarthritis treated with moxibustion

OBJECTIVES:Functional magnetic resonance imaging(fMRI) technology was used to study changes to the resting state blood flow in the brains of patients with knee osteoarthritis(KOA) before and after treatment with moxibustion at the acupoint of the left Dubi(ST 35) and to probe the cerebral mechanism underlying the effect of moxibustion.METHODS:The resting state brain function of 30 patients with left KOA was scanned with fMRI before and after treatment with moxibustion.The analytic methods of fractional amplitude of low frequency fluctuation(fALFF) and regional homogeneity(ReHo) were used to observe changes in resting state brain function.RESULTS:The fALFF values of the right cerebrum,extra-nucleus,left cerebellum,left cerebrum and white matter of patients after moxibustion treatment were higher than before treatment,and the fALFF values of the precentral gyrus,frontal lobe and occipital lobe were lower than before treatment(P<0.05,K≥85).The ReHo values of the thalamus,extra-nucleus and parietal lobe of patients were much higher than those before moxibustion treatment,and the ReHo values of the right cerebrum,left cerebrum and frontal lobe were lower than before treatment(P<0.05,K≥85).CONCLUSION:The influence of moxibustion on obvious changes in brain regions basically conforms to the way that pain and warmth is transmitted in the body,and the activation of sensitive systems in the body may be objective evidence of channel transmission.The regulation of brain function by moxibustion is not in a single brain region but rather in a network of many brain regions.

Magnetic nanoparticles based cancer therapy: current status and applications

Nanotechnology holds a promising potential for developing biomedical nanoplatforms in cancer therapy. The magnetic nanoparticles, which integrate uniquely appealing features of magnetic manipulation, nanoscale heat generator, localized magnetic field and enzyme-mimics, prompt the development and application of magnetic nanoparticles-based cancer medicine. Considerable success has been achieved in improving the magnetic resonance imaging(MRI) sensitivity, and the therapeutic function of the magnetic nanoparticles should be given adequate attention. This work reviews the current status and applications of magnetic nanoparticles based cancer therapy. The advantages of magnetic nanoparticles that may contribute to improved therapeutics efficacy of clinic cancer treatment are highlighted here.