对磁酶免（IEMA）测定血清睾酮的技术改进

目的：研究建立血清睾酮固相试管酶免测定方法。方法：将抗睾酮抗体包被到固相试管壁，血清中的睾酮和睾酮碱性磷酸酶标记物竞争结合固相抗体，标本的浓度通过内插法从标准曲线上求得，并和磁酶免测定法平行对比测定。结果：本法磁酶免测定法进行对比实验并作了直线回归分析，提示两种方法的标本测定结果高度相关，r=0.941，直线回归方程为：Y=0.570+0.835X。配对样本t＝0.090，双侧P＝0．930＞0．05，提示两种检测方法无显著性差异，结论：该方法简便、准确，具有磁酶免法灵敏性和可靠性。

血清孕酮固相酶免测定方法的研究

目的 研究建立血清孕酮固相试管酶免测定方法。方法 将抗孕酮抗体包被到固相试管壁 ,血清中的孕酮标记物竞争结合固相抗体 ,标本的浓度通过内插法从标准曲线上求得 ,并和磁酶免测定法平行对比测定。结果 该种方法和磁酶免测定法进行对比实验并作了直线回归分析 ,提示 2种方法高度相关 ,r=0 .932 ,P <0 .0 0 1;直线回归方程为Y =0 .5 79+0 .892X。两种疗法测定结果经配对t检验 ,P >0 .5 0 ,提示 2种检测方法无显著性差异。结论 该种孕酮固相试管酶免测定方法简便、准确 ,具有较高灵敏性和稳定性。

139对不育夫妇血清生殖激素的测定

目的 以探讨对不育夫妇进行血清生殖激素水平测定的临床价值。方法 本文采用磁酶免激素定量测定方法对来自本站不孕不育门诊的 13 9对不育夫妇进行血清生殖激素定量测定。结果 表明在不育夫妇中 ,男性生殖激素异常占 2 6.6% (3 7/ 13 9) ,女性生殖激素异常者占 43 .9% (61/ 13 9) ,夫妇双方均有异常占 4.9% (7/ 13 9) ,且测定结果表现出多样性和波动性。结论 在不育夫妇间开展血清生殖激素水平测定 ,对病因诊断十分必要 ,并建议成为诊断不育的常规项目。

Nominal effective immunoreaction volume of magnetic beads at single bead level

Immunomagnetic bead（IMB）-based enzyme-linked immunosorbent assay（ELISA） has been the tool frequently used for protein detection in research and clinical laboratories.For most ELISA reactions the recommended dosage of IMBs is usually according to their weight（mg） or mass fraction（w/v） instead of the bead number.Consequently, the processes occurring in the immediate vicinity of the IMBs have always been ignored by researchers and they cannot be revealed in detail during the ELISA reaction.In this paper, we established the relationship between number of IMBs and colorimetric results, and further proposed a new concept of ＂nominal effective immunoreaction volume（NEIV）＂ to characterize a single IMB during ELISA reaction.Results showed that the NEIV of a single IMB has a constant value, which is unrelated to the amount of beads and the concentration of antigen.Optimal results of the colorimetric ELISA are achieved when the incubation volume meets each IMB＇s NEIV and is no longer enhanced by increasing the incubation volume.Thus, the reliable and relatively precise number of IMBs for ELISA detection during practical application could be determined.Most importantly, a study using IMB＇s NEIV would lay the foundation for a kinetics analysis of IMBs and antigens for future study.