Black phosphorus(BP) nanomaterials have attracted extensive attention due to their unique physical,chemical, and biological properties. In this study, small BP nanoparticles were synthesized and modified with dextran ...Black phosphorus(BP) nanomaterials have attracted extensive attention due to their unique physical,chemical, and biological properties. In this study, small BP nanoparticles were synthesized and modified with dextran and poly(ethyleneimine) for functionalization with folic acid and cyanine 7. The functionalized BP nanoparticles exhibit excellent biocompatibility, stability, and near infrared optical properties for targeted imaging of tumors through photoacoustic imaging and near-infrared fluorescence imaging.They also display high photothermal conversion efficiency for photothermal therapy of cancer. This work demonstrates the potential of functionalized small BP nanoparticles as an emerging nanotheranostic agent for the diagnosis and treatment of cancer.展开更多
A label-free and low-cost mapping method based on SERS imaging was reported for illustrating the distribution of phospho- lipids with similar structures in binary lipid membranes on Ag nanoparticles （Ag NPs） films. ...A label-free and low-cost mapping method based on SERS imaging was reported for illustrating the distribution of phospho- lipids with similar structures in binary lipid membranes on Ag nanoparticles （Ag NPs） films. The Ag NPs films exhibited strong SERS activity and good reproducibility which were investigated with p-aminothiopbenol （p-ATP） as probe molecules. Atomic force microscope （AFM） measurement proved that compact lipid membranes formed on the Ag NPs films. Basing on the Ag NPs films, the SERS spectra of phospholipids in the mixed lipid membranes were achieved and the inherent vibration of 1,2-dimyristoyl-sn-lycero-3-phosphoglycerol, sodium salt （DMPG）, 1482 cm-1, was used to distinguish between DMPG and dimyristoylphosphatidylcholine （DMPC）. The proportions of phospholipids in the mixed lipid membranes were represented by the intensity ratio of peaks at 1482 cm-1 and 1650 cm1 （R1482/1650） simultaneously： increasing R1482/1650 indicated higher propor- tion of DMPG and lower proportion of DMPC. SERS imaging of the lipid membranes was constructed as a combination of spacial information and the semiquantitative detection of phospholipids according to R1482/1650, which showed that the charged phospholipids, DMPG, aggregated in the hybrid lipid membranes. The presented mapping strategy based on SERS imaging carried out on Ag NPs films supplied a facile, label-free and inexpensive way for potential applications in the research on the structure of the lipid membrane, such as lipid domains and rafts.展开更多
基金support from the National Natural Science Foundation of China(81471657,81527901)the 1000 Plan for Young Talents,Jiangsu Specially Appointed Professorship+2 种基金Jiangsu Provincial Program for Innovative and Entrepreneurial Talentssupport from the Jiangsu Provincial Key Laboratory of Radiation Medicine and Protectionthe Priority Academic Development Program of Jiangsu Higher Education Institutions(PAPD)
文摘Black phosphorus(BP) nanomaterials have attracted extensive attention due to their unique physical,chemical, and biological properties. In this study, small BP nanoparticles were synthesized and modified with dextran and poly(ethyleneimine) for functionalization with folic acid and cyanine 7. The functionalized BP nanoparticles exhibit excellent biocompatibility, stability, and near infrared optical properties for targeted imaging of tumors through photoacoustic imaging and near-infrared fluorescence imaging.They also display high photothermal conversion efficiency for photothermal therapy of cancer. This work demonstrates the potential of functionalized small BP nanoparticles as an emerging nanotheranostic agent for the diagnosis and treatment of cancer.
基金supported by the National Natural Science Foundation of China (20735003 & 21075120)the National Basic Research Program of China (2009CB930100 & 2010CB933600)
文摘A label-free and low-cost mapping method based on SERS imaging was reported for illustrating the distribution of phospho- lipids with similar structures in binary lipid membranes on Ag nanoparticles （Ag NPs） films. The Ag NPs films exhibited strong SERS activity and good reproducibility which were investigated with p-aminothiopbenol （p-ATP） as probe molecules. Atomic force microscope （AFM） measurement proved that compact lipid membranes formed on the Ag NPs films. Basing on the Ag NPs films, the SERS spectra of phospholipids in the mixed lipid membranes were achieved and the inherent vibration of 1,2-dimyristoyl-sn-lycero-3-phosphoglycerol, sodium salt （DMPG）, 1482 cm-1, was used to distinguish between DMPG and dimyristoylphosphatidylcholine （DMPC）. The proportions of phospholipids in the mixed lipid membranes were represented by the intensity ratio of peaks at 1482 cm-1 and 1650 cm1 （R1482/1650） simultaneously： increasing R1482/1650 indicated higher propor- tion of DMPG and lower proportion of DMPC. SERS imaging of the lipid membranes was constructed as a combination of spacial information and the semiquantitative detection of phospholipids according to R1482/1650, which showed that the charged phospholipids, DMPG, aggregated in the hybrid lipid membranes. The presented mapping strategy based on SERS imaging carried out on Ag NPs films supplied a facile, label-free and inexpensive way for potential applications in the research on the structure of the lipid membrane, such as lipid domains and rafts.
