The study of room temperature phosphorescence (RTP) of several polyaromatic hydrocarbons has been conducted.The results showed that naphthalene,acenaphthene and chrysene absorbed on filter paper can produce strong RTP...The study of room temperature phosphorescence (RTP) of several polyaromatic hydrocarbons has been conducted.The results showed that naphthalene,acenaphthene and chrysene absorbed on filter paper can produce strong RTP by using TlNO 3 as the heavy atom.展开更多
研究发现Ag+为离子微扰剂时3.5代树枝状分子(3.5-G-D)在滤纸固体基质上能发射稳定的室温磷光(RTP).在70℃、15min条件下,基于Cd-3.5-G-D配合物能催化KBrO3氧化3.5-G-D的反应,导致3.5-G-D的RTP信号剧烈猝灭,且△Ip与Cd2+含量成线性关系,...研究发现Ag+为离子微扰剂时3.5代树枝状分子(3.5-G-D)在滤纸固体基质上能发射稳定的室温磷光(RTP).在70℃、15min条件下,基于Cd-3.5-G-D配合物能催化KBrO3氧化3.5-G-D的反应,导致3.5-G-D的RTP信号剧烈猝灭,且△Ip与Cd2+含量成线性关系,据此建立了Cd-3.5-G-D配合物催化KBrO3氧化3.5-G-D固体基质室温磷光法(SS-RTP)测定痕量镉的新方法.该方法灵敏(检出限(LD)为1.2 ag spot–1)、准确、选择性好,适用于生物样品中痕量镉的测定.同时探讨了Cd-3.5-G-D配合物催化SS-RTP测定痕量镉机理.本研究揭开3.5-G-D新的应用领域,发展了新的SS-RTP.展开更多
Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=...Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.展开更多
本研究根据布美他尼(BMTN)抑制Fe3+与桑色素(R)生成络合物[Fe-morin]3+([FeR]3+)而导致体系的室温磷光信号显著猝灭进行的.依据抑制[Fe-桑色素]3+络合物形成效应、BMTN含量与△Ip的线性关系、发展了一种新的测定BMTN的抑制络合反应固体...本研究根据布美他尼(BMTN)抑制Fe3+与桑色素(R)生成络合物[Fe-morin]3+([FeR]3+)而导致体系的室温磷光信号显著猝灭进行的.依据抑制[Fe-桑色素]3+络合物形成效应、BMTN含量与△Ip的线性关系、发展了一种新的测定BMTN的抑制络合反应固体基质室温磷光法(SS-RTP).本方法的检出限为5.0 ag spot-1(对应浓度1.2×10-14 g ml-1),线性范围为0.040-4.0 pg ml-1,显示了高的灵敏度与宽的线性范围,用于人体尿液中BMTN的测定,结果与UV相吻合.同时,用摩尔比法、等摩尔比连续变化法和红外光谱确定络合物的组成为[FeR]3+。展开更多
文摘The study of room temperature phosphorescence (RTP) of several polyaromatic hydrocarbons has been conducted.The results showed that naphthalene,acenaphthene and chrysene absorbed on filter paper can produce strong RTP by using TlNO 3 as the heavy atom.
文摘研究发现Ag+为离子微扰剂时3.5代树枝状分子(3.5-G-D)在滤纸固体基质上能发射稳定的室温磷光(RTP).在70℃、15min条件下,基于Cd-3.5-G-D配合物能催化KBrO3氧化3.5-G-D的反应,导致3.5-G-D的RTP信号剧烈猝灭,且△Ip与Cd2+含量成线性关系,据此建立了Cd-3.5-G-D配合物催化KBrO3氧化3.5-G-D固体基质室温磷光法(SS-RTP)测定痕量镉的新方法.该方法灵敏(检出限(LD)为1.2 ag spot–1)、准确、选择性好,适用于生物样品中痕量镉的测定.同时探讨了Cd-3.5-G-D配合物催化SS-RTP测定痕量镉机理.本研究揭开3.5-G-D新的应用领域,发展了新的SS-RTP.
文摘Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.
文摘本研究根据布美他尼(BMTN)抑制Fe3+与桑色素(R)生成络合物[Fe-morin]3+([FeR]3+)而导致体系的室温磷光信号显著猝灭进行的.依据抑制[Fe-桑色素]3+络合物形成效应、BMTN含量与△Ip的线性关系、发展了一种新的测定BMTN的抑制络合反应固体基质室温磷光法(SS-RTP).本方法的检出限为5.0 ag spot-1(对应浓度1.2×10-14 g ml-1),线性范围为0.040-4.0 pg ml-1,显示了高的灵敏度与宽的线性范围,用于人体尿液中BMTN的测定,结果与UV相吻合.同时,用摩尔比法、等摩尔比连续变化法和红外光谱确定络合物的组成为[FeR]3+。
