急性白血病肝浸润^(31)P磁共振波谱学分析及临床意义

目的:通过对白血病肝脏浸润(LIL)病例与正常对照组的肝脏二维化学位移磁共振31磷波谱成像(2D CSI31P MRS)对照研究,探讨LIL的肝脏磷化合物代谢特征变化。方法:收集LIL15例,并与12例正常肝脏作对照,进行2D CSI31P MRS肝脏扫描,检测各种磷代谢物包括磷酸单酯(PME)、磷酸双酯(PDE)、三磷酸腺苷(ATP)、无机磷(Pi)的相对值,经体模所测校正系数校正后,分析PME、PDE、Pi、ATP的校正相对值(以下简称相对值),及PME/PDE、PME/ATP、PDE/ATP、Pi/ATP、PME/(PME+PDE)的比值变化。结果:在所测的肝脏磷代谢物相对值中,仅LIL组的PME相对值升高,为1.992±0.876,与对照组(1.167±0.427)相比有显著性差异,P<0.05;其它各磷代谢物相对值均无差异。比较LIL与正常对照组之间磷代谢物比值,发现LIL组中与PME相关的比值包括PME/PDE、PME/ATP和PME/(PME+PDE)比值升高,分别为0.551±0.339、1.402±0.654和0.326±0.13,与对照组(分别为0.254±0.059、0.792±0.232和0.199±0.049)相比有显著性差异,P<0.01;LIL组的其它磷代谢物比值与对照组相比均无显著差异。结论:肝31P MRS检查为LIL提供新的非创伤性检测和评价方法,肝脏PME相对值及其相应比值升高提示白血病患者肝浸润存在的可能。

Amplification of UGPase cDNA 3＇ UTR from Saccharum Officinarum

UDP-glucose pyrophosphorylase is an important enzyme concerned with carbohydrate metabolism in plants. Cloning of UGPase is a premise for further study on molecular level, and it is also crucial for study of carbohydrate metabolism. UGPase cDNA sequence as a template, designed primer, then 3＇-untranslate region （3＇ UTR） of UGPase were amplified by 3＇-rapid amplification of cDNA ends （3＇-RACE）. The results suggested the 3＇ UTR were 243 bp, contained AATAA sequence and Poly（A）.

Construction and Expression of Sugarcane UGPase cDNA Prokaryotic Expression Vector

UGPase （UDP-glucose pyrophosphorylase）, one of the primary enzymes concerned with carbohydrate metabolism, catalyzes the formation of UDPG. By inserting the UGPase cDNA fragment cloned from Saccharum officinarum into PQE-30, the prokaryotic expression vector of PQE-UGP was successfully constructed. Then the vector plasmid of PQE-UGP was transformed into host bacteria M 15 and the expression of target gene was induced by Isopropyl β-D-1-Thiogalactopyranoside （IPTG）. The research laid foundation for study on the prokaryotic expression of UGPase.