1.4—二取代酰氨基硫脲类化合物(DATCDS)的植物生理效应和应用——Ⅳ.DATCD—A 对黄瓜生长发育过程中磷化物运输与代谢的影响

应用1.4——二取代酰氨基硫脲化合物 DATCD——A0.001ppm 和水,分别喷施生长发育不同时期的黄瓜整株叶片.<1>幼苗移植含 Na_2H^(32)PO_4(0.2uci/ml)的培养液中,不同时间测定根、茎、叶中^(32)P 化物的放射活性,DATCD—A 处理的植株,促进^(32)P 的吸收,其中,整株,叶片和叶子中的^(32)P 脉冲数是水处理的2.5倍、5.7倍和8.2倍;糖和酸性有机磷含量,以及光合磷酸化活力、ATP 生成量均比水处理高而核蛋白含量低于对照.<2>结果期,在叶腋着生果实相同部位的叶片上涂前放射强度的^(32)P 溶液,随者时间的延长,24～96小时 DATCD——A 处理的植株果实内^(32)P 化物的放射活性同水处理的植株相比,增加46.30～278.65%.DATCD——A*1—异烟酰基—4—(4′—氯代苯基)

Amplification of UGPase cDNA 3＇ UTR from Saccharum Officinarum

UDP-glucose pyrophosphorylase is an important enzyme concerned with carbohydrate metabolism in plants. Cloning of UGPase is a premise for further study on molecular level, and it is also crucial for study of carbohydrate metabolism. UGPase cDNA sequence as a template, designed primer, then 3＇-untranslate region （3＇ UTR） of UGPase were amplified by 3＇-rapid amplification of cDNA ends （3＇-RACE）. The results suggested the 3＇ UTR were 243 bp, contained AATAA sequence and Poly（A）.

Construction and Expression of Sugarcane UGPase cDNA Prokaryotic Expression Vector

UGPase （UDP-glucose pyrophosphorylase）, one of the primary enzymes concerned with carbohydrate metabolism, catalyzes the formation of UDPG. By inserting the UGPase cDNA fragment cloned from Saccharum officinarum into PQE-30, the prokaryotic expression vector of PQE-UGP was successfully constructed. Then the vector plasmid of PQE-UGP was transformed into host bacteria M 15 and the expression of target gene was induced by Isopropyl β-D-1-Thiogalactopyranoside （IPTG）. The research laid foundation for study on the prokaryotic expression of UGPase.