采用常规射频等离子体增强化学气相沉积方法,以高氢稀释的Si H4为源气体和以PH3为掺杂剂,制备了磷掺杂的氢化纳米晶硅薄膜。结果表明,薄膜的生长速率随PH3/Si H4流量比（Cp）增加而显著减小。Raman谱的研究证实,随Cp增加,薄膜的晶化率...采用常规射频等离子体增强化学气相沉积方法,以高氢稀释的Si H4为源气体和以PH3为掺杂剂,制备了磷掺杂的氢化纳米晶硅薄膜。结果表明,薄膜的生长速率随PH3/Si H4流量比（Cp）增加而显著减小。Raman谱的研究证实,随Cp增加,薄膜的晶化率经历了先增大后减小的过程,当Cp=1.0%,晶化率达到最大值45.9%。傅里叶变换红外吸收谱测量结果显示,薄膜中的H含量在Cp=2.0%时达到最低值9.5%。光学测量结果表明,本征和掺P的氢化纳米晶硅薄膜在可见光谱范围呈现出良好的光吸收特性,在0.8~3.0 e V范围内,nc-Si（P）∶H薄膜的吸收系数显著大于c-Si。和α-Si∶H薄膜相比,虽然短波范围的吸收系数较低,但是在hν〈1.7 e V区域,nc-Si（P）∶H薄膜的吸收系数要高两到三个量级,显示出优良的红光响应。电学测量表明,适当掺P会显著提高氢化纳米晶硅薄膜的暗电导率,当Cp=0.5%时,薄膜的暗电导率可达5.4 S·cm-1。展开更多
OBJECTIVE To investigate the damage effect and mechanisms of cyclophosphamide(CTX)and its active metabolite derivative 4-hydroperoxycyclophosphamide(4-HC)to human neuroblas⁃toma SH-SY5Y cells.METHODS SH-SY5Y cells wer...OBJECTIVE To investigate the damage effect and mechanisms of cyclophosphamide(CTX)and its active metabolite derivative 4-hydroperoxycyclophosphamide(4-HC)to human neuroblas⁃toma SH-SY5Y cells.METHODS SH-SY5Y cells were treated with CTX[0(cell control),0.01,0.1,1,5,10,20,40 and 80 mmol·L^(-1)]and 4-HC[0(cell control),0.01,0.1,1,5,10,20,40 and 80μmol·L^(-1)]for 48 h.Cell confluence and morphology were observed by the IncuCyte ZOOM system.Cell viability was assessed by CCK-8 assay.Lactate dehydrogenase(LDH)release was measured by LDH assay kit.SH-SY5Y cells were treated with CTX(0,1,5,10 and 20 mmol·L^(-1))and 4-HC(0,1,5,10 and 20μmol·L^(-1))for 48 h before cell proliferation was analyzed by 5-ethynyl-2′-deoxyuridine(EdU)staining assay.Immunofluorescence was employed to assess the levels of the DNA double-strand break markerγ-H2AX and to evaluate changes in mitochondrial membrane potential.SH-SY5Y cells were treated with CTX(0,1,5 and 10 mmol·L^(-1))and 4-HC(0,1,5 and 10μmol·L^(-1))for 48 h,and the alterations in glycolysis and oxidative phosphorylation levels were analyzed using the Seahorse XFe96 Analyzer.RESULTS Compared with the cell control group,cell confluence and cell viability were significantly reduced in the CTX and 4-HC groups(P<0.01),and the half-maximal inhibitory concentrations(IC50)for CTX and 4-HC were 4.44 mmol·L^(-1) and 4.78μmol·L^(-1),respectively.The release rate of LDH was signif⁃icantly increased while the percentage of EdU+cells was significantly reduced in the CTX and 4-HC groups(P<0.01).The percentage ofγ-H2AX+cells was significantly increased and mitochondrial membrane potential significantly decreased in the CTX and 4-HC group(P<0.05).Treatment with CTX and 4-HC resulted in reduced levels of maximum glycolytic capacity,glycolytic reserve,maximal respi⁃ration,and ATP production(P<0.05).CONCLUSION CTX and 4-HC exert significant cytotoxic effects on SH-SY5Y cells by disrupting cell membrane structure,impeding cell proliferation,and reducing cell viability.The mechanisms underlying these effects may involve intracellular DNA damage,disturbance of energy metabolism and mitochondrial dysfunction.展开更多
文摘采用常规射频等离子体增强化学气相沉积方法,以高氢稀释的Si H4为源气体和以PH3为掺杂剂,制备了磷掺杂的氢化纳米晶硅薄膜。结果表明,薄膜的生长速率随PH3/Si H4流量比（Cp）增加而显著减小。Raman谱的研究证实,随Cp增加,薄膜的晶化率经历了先增大后减小的过程,当Cp=1.0%,晶化率达到最大值45.9%。傅里叶变换红外吸收谱测量结果显示,薄膜中的H含量在Cp=2.0%时达到最低值9.5%。光学测量结果表明,本征和掺P的氢化纳米晶硅薄膜在可见光谱范围呈现出良好的光吸收特性,在0.8~3.0 e V范围内,nc-Si（P）∶H薄膜的吸收系数显著大于c-Si。和α-Si∶H薄膜相比,虽然短波范围的吸收系数较低,但是在hν〈1.7 e V区域,nc-Si（P）∶H薄膜的吸收系数要高两到三个量级,显示出优良的红光响应。电学测量表明,适当掺P会显著提高氢化纳米晶硅薄膜的暗电导率,当Cp=0.5%时,薄膜的暗电导率可达5.4 S·cm-1。
文摘OBJECTIVE To investigate the damage effect and mechanisms of cyclophosphamide(CTX)and its active metabolite derivative 4-hydroperoxycyclophosphamide(4-HC)to human neuroblas⁃toma SH-SY5Y cells.METHODS SH-SY5Y cells were treated with CTX[0(cell control),0.01,0.1,1,5,10,20,40 and 80 mmol·L^(-1)]and 4-HC[0(cell control),0.01,0.1,1,5,10,20,40 and 80μmol·L^(-1)]for 48 h.Cell confluence and morphology were observed by the IncuCyte ZOOM system.Cell viability was assessed by CCK-8 assay.Lactate dehydrogenase(LDH)release was measured by LDH assay kit.SH-SY5Y cells were treated with CTX(0,1,5,10 and 20 mmol·L^(-1))and 4-HC(0,1,5,10 and 20μmol·L^(-1))for 48 h before cell proliferation was analyzed by 5-ethynyl-2′-deoxyuridine(EdU)staining assay.Immunofluorescence was employed to assess the levels of the DNA double-strand break markerγ-H2AX and to evaluate changes in mitochondrial membrane potential.SH-SY5Y cells were treated with CTX(0,1,5 and 10 mmol·L^(-1))and 4-HC(0,1,5 and 10μmol·L^(-1))for 48 h,and the alterations in glycolysis and oxidative phosphorylation levels were analyzed using the Seahorse XFe96 Analyzer.RESULTS Compared with the cell control group,cell confluence and cell viability were significantly reduced in the CTX and 4-HC groups(P<0.01),and the half-maximal inhibitory concentrations(IC50)for CTX and 4-HC were 4.44 mmol·L^(-1) and 4.78μmol·L^(-1),respectively.The release rate of LDH was signif⁃icantly increased while the percentage of EdU+cells was significantly reduced in the CTX and 4-HC groups(P<0.01).The percentage ofγ-H2AX+cells was significantly increased and mitochondrial membrane potential significantly decreased in the CTX and 4-HC group(P<0.05).Treatment with CTX and 4-HC resulted in reduced levels of maximum glycolytic capacity,glycolytic reserve,maximal respi⁃ration,and ATP production(P<0.05).CONCLUSION CTX and 4-HC exert significant cytotoxic effects on SH-SY5Y cells by disrupting cell membrane structure,impeding cell proliferation,and reducing cell viability.The mechanisms underlying these effects may involve intracellular DNA damage,disturbance of energy metabolism and mitochondrial dysfunction.
