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氮、磷源浓度对裂殖壶菌生长及油脂积累的影响 被引量:2
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作者 范学宁 成艳 +1 位作者 赵国服 陈明 《食品与发酵工业》 CAS CSCD 北大核心 2022年第24期189-194,共6页
裂殖壶菌是替代深海鱼油产业化生产二十二碳六烯酸(docosahexaenoic acid,DHA)油脂的极具潜力的微生物。该研究采用1株裂殖壶菌DP-16发酵产油脂,以葡萄糖为碳源,以酵母浸粉和谷氨酸钠为氮源,以KH 2PO 4为磷源,探究了氮、磷源浓度对菌体... 裂殖壶菌是替代深海鱼油产业化生产二十二碳六烯酸(docosahexaenoic acid,DHA)油脂的极具潜力的微生物。该研究采用1株裂殖壶菌DP-16发酵产油脂,以葡萄糖为碳源,以酵母浸粉和谷氨酸钠为氮源,以KH 2PO 4为磷源,探究了氮、磷源浓度对菌体生长、葡萄糖消耗和油脂积累的影响。结果显示,在正常氮、磷源浓度下培养菌体,0~24 h为细胞增殖期,24~84 h为油脂积累期,在84 h油脂产量和油脂含量分别达到14.1 g/L和33.5%,84~96 h进入油脂反耗期。改变氮、磷源浓度,低磷条件下72 h的油脂产量和油脂含量分别达到14.8 g/L和44.3%,比对照组分别提高了5.0%和32.2%,而高氮、低氮、高磷等条件均不利于裂殖壶菌产油脂。研究结果表明,氮、磷源浓度对裂殖壶菌细胞生长和油脂积累产生重要影响,油脂产量取决于菌体生物量和细胞内油脂含量的乘积,为提高油脂产量,需要在获得高细胞浓度(生物量)和达到高油脂含量之间找到一个合适的平衡,以确定适宜的氮、磷源浓度。该研究对促进裂殖壶菌发酵产DHA油脂具有重要参考意义。 展开更多
关键词 裂殖壶菌 DHA油脂 氮源浓度 磷源浓度 细胞生长 油脂积累
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Interactions Between Exogenous Rare Earth Elements and Phosphorus Leaching in Packed Soil Columns 被引量:2
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作者 LIANG Tao SONG Wen-Chong +2 位作者 WANG Ling-Qing P. J. A. KLEINMAN CAO Hong-Ying 《Pedosphere》 SCIE CAS CSCD 2010年第5期616-622,共7页
Rare earth elements (REEs) increasingly used in agriculture as an amendment for crop growth may help to lessen environmental losses of phosphorus (P) from heavily fertilized soils. The vertical transport character... Rare earth elements (REEs) increasingly used in agriculture as an amendment for crop growth may help to lessen environmental losses of phosphorus (P) from heavily fertilized soils. The vertical transport characteristics of P and REEs, lanthanum (La), neodymium (Nd), samarium (Sin), and cerium (Ce), were investigated with addition of exogenous REEs at various doses to packed soil columns (20 cm deep). Vertical transfers of REEs and P were relatively small, with transport depths less than 6 cm for most REEs and P. Export of applied REEs in leachate accounted for less that 5% of inputs. The addition of Ce, Nd and Sm to soil columns significantly decreased concentrations of extractable soil P up to a depth of 4 cm, with soil P concentrations unaffected at depths 〉 4 cm. In general, REEs had little effect on the vertical leaching of P in packed soil columns. 展开更多
关键词 extractable soil P MANURE MOBILITY TRANSLOCATION vertical transport
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