The supported bilayer lipid membrane(s-BLM) was served as a biomembrane model. The interaction between sodium cholate(NaC) and s-BLM was investigated by cyclic voltammetry and alternating current(AC) impedance spectro...The supported bilayer lipid membrane(s-BLM) was served as a biomembrane model. The interaction between sodium cholate(NaC) and s-BLM was investigated by cyclic voltammetry and alternating current(AC) impedance spectroscopy. The following conclusions were obtained: NaC can decrease the orderliness of lecithin molecule arrangement and induce pores or defects on s-BLM and the interaction between them depends on time, concentration and cholesterol. In addition, the defective s-BLM after interaction can be self-repaired in 0.1 mol/L KCl solution, which indicates that the interaction is reversible. Moreover, a possible mechanism of the interaction was proposed finally.展开更多
Avidin was immobilized on chemically oxidized silicon substrate by means of the surface self-assembling method,and consecutive tethered lipid bilayer membrane was formed after vesicle fusion on such modified substrate...Avidin was immobilized on chemically oxidized silicon substrate by means of the surface self-assembling method,and consecutive tethered lipid bilayer membrane was formed after vesicle fusion on such modified substrate.The structures of this tethered membrane were investigated by atomic force microscopy(AFM).Due to the introduction of a 5 nm water cushion layer between the lipid bilayer and the substrate,a ripple phase was observed in this tethered membrane.The structure of such tethered membrane was different from the simple supported bilayer.These experimental observations demonstrated that the thickness increase of water-layer could effectively reduce the interaction between lipid bilayer and substrate.展开更多
文摘The supported bilayer lipid membrane(s-BLM) was served as a biomembrane model. The interaction between sodium cholate(NaC) and s-BLM was investigated by cyclic voltammetry and alternating current(AC) impedance spectroscopy. The following conclusions were obtained: NaC can decrease the orderliness of lecithin molecule arrangement and induce pores or defects on s-BLM and the interaction between them depends on time, concentration and cholesterol. In addition, the defective s-BLM after interaction can be self-repaired in 0.1 mol/L KCl solution, which indicates that the interaction is reversible. Moreover, a possible mechanism of the interaction was proposed finally.
文摘Avidin was immobilized on chemically oxidized silicon substrate by means of the surface self-assembling method,and consecutive tethered lipid bilayer membrane was formed after vesicle fusion on such modified substrate.The structures of this tethered membrane were investigated by atomic force microscopy(AFM).Due to the introduction of a 5 nm water cushion layer between the lipid bilayer and the substrate,a ripple phase was observed in this tethered membrane.The structure of such tethered membrane was different from the simple supported bilayer.These experimental observations demonstrated that the thickness increase of water-layer could effectively reduce the interaction between lipid bilayer and substrate.
基金supported by Discovery grant from the Natural Sciences and Engineering Research Council of Canada.J.L.support from the Canada Research Chairs (CRC) program
