The plant phospholipase D （PLD） plays versatile functions in multiple aspects of plant growth, development, and stress re- sponses. However, until now, our knowledge concerning the PLD gene family members and their ...The plant phospholipase D （PLD） plays versatile functions in multiple aspects of plant growth, development, and stress re- sponses. However, until now, our knowledge concerning the PLD gene family members and their expression patterns in cotton has been limited. In this study, we performed for the first time the genome-wide analysis and expression profiling of PLD gene family in Gossypium arboretum, and finally, a total of 19 non-redundant PLD genes （GaPLDs） were identified. Based on the phylogenetic analysis, they were divided into six well-supported clades （tx, 13/？, 8, ~, ~ and q~）. Most of the GaPLD genes with- in the same clade showed the similar exon-intron organization and highly conserved motif structures. Additionally, the chro- mosomal distribution pattern revealed that GaPLD genes were unevenly distributed across 10 of the 13 cotton chromosomes. Segmental duplication is the major contributor to the expansion of GaPLD gene family and estimated to have occurred from 19.61 to 20.44 million years ago when a recent large-scale genome duplication occurred in cotton. Moreover, the expression profiling provides the functional divergence of GaPLD genes in cotton and provides some new light on the molecular mecha- nisms of GaPLDcd and GaPLD62 in fiber development.展开更多
基金the State Key Basic Research and Development Plan (2010CB126003)the National Transgenic Animals and Plants Research Project (2011ZX08005-003, 2011ZX08009-003)
文摘The plant phospholipase D （PLD） plays versatile functions in multiple aspects of plant growth, development, and stress re- sponses. However, until now, our knowledge concerning the PLD gene family members and their expression patterns in cotton has been limited. In this study, we performed for the first time the genome-wide analysis and expression profiling of PLD gene family in Gossypium arboretum, and finally, a total of 19 non-redundant PLD genes （GaPLDs） were identified. Based on the phylogenetic analysis, they were divided into six well-supported clades （tx, 13/？, 8, ~, ~ and q~）. Most of the GaPLD genes with- in the same clade showed the similar exon-intron organization and highly conserved motif structures. Additionally, the chro- mosomal distribution pattern revealed that GaPLD genes were unevenly distributed across 10 of the 13 cotton chromosomes. Segmental duplication is the major contributor to the expansion of GaPLD gene family and estimated to have occurred from 19.61 to 20.44 million years ago when a recent large-scale genome duplication occurred in cotton. Moreover, the expression profiling provides the functional divergence of GaPLD genes in cotton and provides some new light on the molecular mecha- nisms of GaPLDcd and GaPLD62 in fiber development.
