慢性肾脏病-矿物质和骨代谢异常(chronic kidney disease-mineral and bone disorder,CKD-MBD)是慢性肾脏病(chronic kidney disease,CKD)患者常见的并发症之一,控制高磷血症为防治CKD-MBD的关键。然而,药物作为慢性肾脏病患者磷肠道负...慢性肾脏病-矿物质和骨代谢异常(chronic kidney disease-mineral and bone disorder,CKD-MBD)是慢性肾脏病(chronic kidney disease,CKD)患者常见的并发症之一,控制高磷血症为防治CKD-MBD的关键。然而,药物作为慢性肾脏病患者磷肠道负荷的潜在来源却常常被忽视。故本文针对药物的磷含量、低磷/蛋白质比值饮食的研究进展予以综述。展开更多
Objective: To analyze and identify differentially expressed phosphoproteins associated with mitochondrial KATP channel opening. Methods: Adult rat ventricular myocytes were isolated, cultured, and identified, and pret...Objective: To analyze and identify differentially expressed phosphoproteins associated with mitochondrial KATP channel opening. Methods: Adult rat ventricular myocytes were isolated, cultured, and identified, and pretreated without or with 100μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched from the control and diazoxide-pretreated cells were separated by two-dimensional gel elec-trophoresis (2-DE) followed by sliver staining. The obtained interesting phosphoproteins were further i-dentified by mass spectrometry. Results: Associated with diazoxide preconditioning, the proteins of chap-eronin containing TCP-1 and hypothetical protein XP- 346548 were phosphorylated significantly (P< 0. 01), while the 94-kDa glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphory-lated markedly (P<0. 01). Conclusion: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in order to respond diazoxide preconditioning, and these phosphorylated protein may mediate the downstream signaling of cardioprotec-tion by mitochondrial KATP channel opening induced by ischemic preconditioning.展开更多
文摘慢性肾脏病-矿物质和骨代谢异常(chronic kidney disease-mineral and bone disorder,CKD-MBD)是慢性肾脏病(chronic kidney disease,CKD)患者常见的并发症之一,控制高磷血症为防治CKD-MBD的关键。然而,药物作为慢性肾脏病患者磷肠道负荷的潜在来源却常常被忽视。故本文针对药物的磷含量、低磷/蛋白质比值饮食的研究进展予以综述。
基金Supported by the National Natural Science Foundation of China (No. 30200089 and No. 30500211)
文摘Objective: To analyze and identify differentially expressed phosphoproteins associated with mitochondrial KATP channel opening. Methods: Adult rat ventricular myocytes were isolated, cultured, and identified, and pretreated without or with 100μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched from the control and diazoxide-pretreated cells were separated by two-dimensional gel elec-trophoresis (2-DE) followed by sliver staining. The obtained interesting phosphoproteins were further i-dentified by mass spectrometry. Results: Associated with diazoxide preconditioning, the proteins of chap-eronin containing TCP-1 and hypothetical protein XP- 346548 were phosphorylated significantly (P< 0. 01), while the 94-kDa glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphory-lated markedly (P<0. 01). Conclusion: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in order to respond diazoxide preconditioning, and these phosphorylated protein may mediate the downstream signaling of cardioprotec-tion by mitochondrial KATP channel opening induced by ischemic preconditioning.
