豚鼠鼓室灌注给药测定外淋巴液中磷酸地塞米松的含量

目的建立豚鼠外耳淋巴液中磷酸地塞米松的含量测定方法。方法采用听泡灌注给药，在给药结束后30min采集鼓阶外淋巴液，应用高效液相色谱仪测定磷酸地基米松的含量．HPLC色谱条件为ZORBAX Eclipse XDB-C18色谱柱（4．6ml×250mm．5μm），流动相为7．56mmol·L^-1硫酸铵-乙腈（70：30），流速1．0ml·min^-1，柱温20℃，紫外检测波长239nm．结果磷酸地塞米松的线性范围为0．10～4.8mg·L^-1（r=0．9998）。最低检测限为6．25μg·L^-1，日内和日间RSD分别为2．17％和3．25％。结束给药30min后鼓阶外淋巴液中磷酸地塞米松的浓度为2．20mg·L^-1．结论方法灵敏、准确，重现性好，可用于外淋巴液中磷酸地塞米松的测定。

豚鼠鼓室明胶海绵吸附给药外淋巴液中磷酸地塞米松的含量测定

目的:建立豚鼠外耳淋巴液中磷酸地塞米松的含量测定方法。方法:采用浸满磷酸地塞米松溶液的明胶海绵颗粒置于圆窗龛内,采集给药1 h后鼓阶外淋巴液。HPLC色谱条件为,ZORBAX Eclipse XDB-C18色谱柱(4．6 mm×250 mm,5 μm),流动相为7．56 mmol·L-1硫酸铵-乙腈(70:30),流速1．0 ml·min-1,柱温20℃,紫外检测波长239 nm。结果:磷酸地塞米松的线性范围为0．10-4．8 mg·L-1(r=0．999 8)。最低检测限为6．25 μg·L-1,日内和日间RSD分别为1．7％和2．3％。给药1 h后鼓阶外淋巴液中磷酸地塞米松的浓度为2．12 mg·L-1。结论:该法灵敏度高,准确性、重复性好,可用于外淋巴液中磷酸地塞米松的测定。

高效液相色谱法测定色甘酸钠地塞米松滴鼻液含量

目的建立一种HPLC法同时测定地塞米松色甘酸钠滴鼻液中磷酸地塞米松和色甘酸钠含量.方法采用Diamonsil C18(150 mm×4.6 mm,5μm)色谱柱,流动相:A:缓冲液(0.01 mol/L磷酸二氢钾,pH为4.7),B:甲醇,0～5 min:A-B(42:58),6 min后A-B(30:70),流速:1 mL/min,柱温:25℃,检测波长:240 nm.外标法计算含量.结果在该色谱条件下,磷酸地塞米松和色甘酸钠线性良好(r＞0.999,n=6),加样回收率为98%～102.55%(n=3),RSD＜2.0%(n=3).样品在8 h内稳定.测得样品含量合格,在97%～103%.结论经方法学实验验证,该方法简便,快速,结果准确,可靠,重现性好.

中西医结合治疗过敏性皮炎49例疗效观察

目的:观察中西医结合治疗过敏性皮炎的临床疗效。方法:41例病例中,轻型28例,单纯采用西药治疗,应用维生素C注射液2 g,葡萄糖酸钙注射液10 mL、磷酸地塞米松注射液5 mg～20 mg两种或三种药物静脉推注,溴化钙注射液20 mL、复方甘草酸苷注射液40 mL～60 mL静脉滴注;中、重型共21例,采用中西医结合的方法治疗。结果:49例病例其中7例仅表现为荨麻疹,中途终止治疗,余41例病例中38例痊愈,3例皮疹消退,但仍有季节性瘙痒。结论:中西医结合治疗过敏性皮炎有较好的疗效,值得临床推广。

Crystal Growth Models of Dexamethasone Sodium Phosphate in a MSMPR Reactive Crystallizer

The reactive crystallization process of dexamethasone sodium phosphate was investigated in a continuous mixed-suspension, mixed-product-removal(MSMPR) crystallizer. Analyzing experimental data, it was found that the growth of product crystal was size-dependent. The Bransom, CR, ASL, M J2 and M J3 size-dependent growth models were discussed in details. Using experimental steady state population density data of dexamethasone sodium phosphate, parameters of five size-dependent growth models were determined by the method of non-linear least-squares. By comparison of experimental population density and linear growth rate data with those obtained from the five size-dependent growth models, it was found that the MJ3 model predicts the growth more accurately than do the other four models. Based on the theory of population balance, the crystal nucleation and growth rate equations of dexamethasone sodium phosphate were determined by non-linear regression method. The effects of different operation parameters such as supersaturation, magma density and temperature on the quality of product crystal were also discussed, and the optimal operation conditions were derived.

Clinical effect of Jiutengzhuyu tablets on promoting blood circulation in women with oviducal obstruction

OBJECTIVE： To test the clinical curative effect of Jiutengzhuyu tablets, a patented Chinese vine-de- rived drug used for promoting blood circulation, on women with oviducal obstruction. METHODS Patients with tubal infertility were di- vided randomly into two groups： 58 patients in the treatment group and 57 in a control group. The treated patients took Jiutengzhuyu tablets orally for 25 days. The control group received an intrauter- ine infusion of 5 mg dexamethasone sodium phos- phate, 4000 units of chymotrypsin, 80 000 units of gentamicin sulfate dissolved in 20 mL of normal sa- line at I mL/min. After 3 months of treatment, the curative effect on tubal patency was assessed and a l-year follow-up visit was used to document any pregnancies. RESULTS The total effective rate was 78% in the treatment group and 32% in the control group with a statistically significant difference （x2=24.57, P〈0.01）.CONCLUSION： Jiutengzhuyu was effective in treat- ing infertility caused by tubal infertility with a Traditional Chinese Medicine diagnosis of blood stasis.