Cytosolic phospholipase A2α （cPLA2α） catalyzes the release of arachidonic acid （AA） and lysophosphoglyceride from membrane phospholipids. Although the roles of AA and eicosanoids in cellular viability, the proce...Cytosolic phospholipase A2α （cPLA2α） catalyzes the release of arachidonic acid （AA） and lysophosphoglyceride from membrane phospholipids. Although the roles of AA and eicosanoids in cellular viability, the processes of inflammation and cancer cell development have been extensively studied, the function of cPLA2α in the processes of inflammation and cancer cell development is not clear. This review summarizes published evidences for the biochemical properties and regulatory mechanisms of cPLA2α. The potential for use of cPLA2α as a novel diagnostic target and predictive biomarker for tumors is also discussed.展开更多
DHA （docosahexaenoic acid） and EPA （eicosapentaenoic acid） induced intracellular calcium increase in taste receptor cells purified from mouse circumvallate papillae. We have demonstrated that DHA and EPA induced c...DHA （docosahexaenoic acid） and EPA （eicosapentaenoic acid） induced intracellular calcium increase in taste receptor cells purified from mouse circumvallate papillae. We have demonstrated that DHA and EPA induced calcium increase respectively via cPLA2 （cytosolic phospholipase A2） type lVc and calcium independent iPLA2 （phospholipase A2） type VI. Intracellular calcium increases via PLC （phospholipase C） pathway and leads to a calcium effiux and further SOC （stored operated calcium） channels activation. An abundant increase in intracellular calcium will induce exocytosis of neurotransmitters into the extracellular medium. Our results suggest that DHA and EPA induced calcium signaling, and should be implicated in the transmission of lipid gustatory information to afferent nerve fibers, as shown previously with linoleic acid.展开更多
文摘Cytosolic phospholipase A2α （cPLA2α） catalyzes the release of arachidonic acid （AA） and lysophosphoglyceride from membrane phospholipids. Although the roles of AA and eicosanoids in cellular viability, the processes of inflammation and cancer cell development have been extensively studied, the function of cPLA2α in the processes of inflammation and cancer cell development is not clear. This review summarizes published evidences for the biochemical properties and regulatory mechanisms of cPLA2α. The potential for use of cPLA2α as a novel diagnostic target and predictive biomarker for tumors is also discussed.
文摘DHA （docosahexaenoic acid） and EPA （eicosapentaenoic acid） induced intracellular calcium increase in taste receptor cells purified from mouse circumvallate papillae. We have demonstrated that DHA and EPA induced calcium increase respectively via cPLA2 （cytosolic phospholipase A2） type lVc and calcium independent iPLA2 （phospholipase A2） type VI. Intracellular calcium increases via PLC （phospholipase C） pathway and leads to a calcium effiux and further SOC （stored operated calcium） channels activation. An abundant increase in intracellular calcium will induce exocytosis of neurotransmitters into the extracellular medium. Our results suggest that DHA and EPA induced calcium signaling, and should be implicated in the transmission of lipid gustatory information to afferent nerve fibers, as shown previously with linoleic acid.
