[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,...[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,Selenomonas ruminantium（recipient strain）was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.[Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.[Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal.展开更多
基金Supported by National Natural Science Fund Item(30230260,30600441)~~
文摘[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,Selenomonas ruminantium（recipient strain）was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.[Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.[Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal.
