目的观察大鼠酪氨酸羟化酶（tyrosinehydroxylase，TH）修饰的骨髓基质源性神经元样干细胞（neuronoid stem cells derived from bone marrow stem cells，NdSCs-D—BMSCs）在脑室移植途径下对帕金森病（Parkinson disease，PD）大鼠的...目的观察大鼠酪氨酸羟化酶（tyrosinehydroxylase，TH）修饰的骨髓基质源性神经元样干细胞（neuronoid stem cells derived from bone marrow stem cells，NdSCs-D—BMSCs）在脑室移植途径下对帕金森病（Parkinson disease，PD）大鼠的治疗作用。方法将酶切鉴定后的新构建质粒pEGFP—C2-TH经电穿孔法转染培养第8天NdSCs—D—BMSCs，注射到PD大鼠模型右侧脑室，观察大鼠行为学变化，移植细胞在大鼠脑组织内的迁移，以及高效液相方法检测脑内DA含量。结果质粒pEGFP—C2-TH转染NdSCs—D—BMSCs移植后10周，PD大鼠症状显著改善，DA恢复至正常水平33．0％，移植细胞可以在PD大鼠脑内存活，并出现远处迁移。结论TH修饰的大鼠NdSCs—D—BMSCs经脑室移植对PD大鼠具有明显的治疗作用，为临床中腰椎穿刺干细胞移植的应用提供实验依据。展开更多
Objective To probe into the function mechanism of penetration therapy with head electrical acupuncture on Parkinson＇s disease. Methods Microinjection of 6-hydroxydopamin （6-OHDA） on the left cor- pus striatum was a...Objective To probe into the function mechanism of penetration therapy with head electrical acupuncture on Parkinson＇s disease. Methods Microinjection of 6-hydroxydopamin （6-OHDA） on the left cor- pus striatum was adopted to prepare rotation model of Parkinson^s disease in rat. Penetration therapy with head electrical acupuncture was administered in treatment. Normal group, sham-operation group, model group and penetration therapy group were set up. （1）lmmunohistochemical （IHC） method was used to test the morphology and count of positive cell of tyrosine hydroxylase （TH）. （2）RT-PCR technology was used to detect the expression of nestin mRNA of neural stem cell （NSC）. Results （1）Compared with model group, in pene- tration therapy group, the expressions of TH-positive neurons in immune response were increased in areal density （AD）, numerical density （ND） and integrating optic density （P〈0.05）. （2）Compared with model group, in penetration therapy group, the expression of nestin mRNA was increased （P〈0. 05）. Conclusion Penetration therapy with head electrical acupuncture promotes the proliferation of endogenous neural stem cells in substantia nigra of rat model of Parkinson＇s disease.展开更多
Neural stem cell is presently the research hotspot in neuroscience. Recent progress indicates that epigenetic modulation is closely related to the self-renewal and differentiation of neural stem cell. Epigenetics refe...Neural stem cell is presently the research hotspot in neuroscience. Recent progress indicates that epigenetic modulation is closely related to the self-renewal and differentiation of neural stem cell. Epigenetics refer to the study of mitotical/meiotical heritage changes in gene function that cannot be explained by changes in the DNA sequence. Major epigenetic mechanisms include DNA methylation, histone modification, chromatin remodeling, genomic imprinting, and non-coding RNA. In this review, we focus on the new insights into the epigenetic mechanism for neural stem cells fate.展开更多
Stroke has been a common problem in the global public health because of its high incidence,recur-rence,disability rate and mortality,among which ischemic stroke accounts for about 80%of the total stroke.Cerebral ische...Stroke has been a common problem in the global public health because of its high incidence,recur-rence,disability rate and mortality,among which ischemic stroke accounts for about 80%of the total stroke.Cerebral ischemia reperfusion injury(CIRI)is one of the most important pathological injuries after ischemic stroke,and the effective treatment has always been a research focus at home and abroad.The proposal of the concept of neurovascular unit(NVU)which extends treatment ideas from local to holistic is in accordance with the holistic concept and treatment based on syndrome differentiation in Chinese medicine.Chinese medicine has many advantages,such as being multicomponent,multitarget,and multipath while stem cells are effective biological therapeutic means,both of which have been proved to exert comprehensive and definite effects on NVU injury caused by CIRI.This artide reviews the molecular mechanisms of NVU injury and the cerebropro-tective effects of Chinese medicine monomers or compound formulas combined with MSCs on NUV injury after cerebral ischemia,with a view to provide a theoretical basis for future studies.展开更多
OBJECTIVE: To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS: Velvet antler(Cervus Nippon Temminck) polypeptide(VAP) was used to differentiate neural stem cells(NSCs) towa...OBJECTIVE: To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS: Velvet antler(Cervus Nippon Temminck) polypeptide(VAP) was used to differentiate neural stem cells(NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immuno fl uorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.RESULTS: The differentiating cells look like neuron,some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.CONCLUSION: Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.展开更多
Objective： To explore the electrophysiological proper- ties of differentiation of rat bone marrow-derived stromal stem cells （rBMSCs） to neuron-like cells in vitro by edaravone, a new type of free radical scavenger...Objective： To explore the electrophysiological proper- ties of differentiation of rat bone marrow-derived stromal stem cells （rBMSCs） to neuron-like cells in vitro by edaravone, a new type of free radical scavenger. Methods： Stromal stem cells were separated from rat bone marrow with Ficoll-Paque reagent and expanded in different culture medium in vitro, rBMSCs were induced by edaravone containing serum-free L-DMEM. Morphologic observation and Western blot analysis including the expression of Nav1.6, Kvl.2, Kv1.3, Cav1.2 were performed, and whole patch-clamp technique was used. Results： Cyton contraction and long processes were shown in differentiated stromal stem cells. Navl.6, Kvl.2, Kv 1.3 and Cav 1.2 were expressed in both differentiated and undifferentiated ceils. However, the expression of channel proteins in differentiated cells was up-regulated. Consistently, their resting potential and outward currents were also enhanced in the differentiated cells, which was especially significant in the outward rectifier potassium current. Conclusion： In vitro, neuron-like cells derived from rBMSCs, induced by edaravone, possess electrophysiological properties of neurons.展开更多
文摘目的观察大鼠酪氨酸羟化酶（tyrosinehydroxylase，TH）修饰的骨髓基质源性神经元样干细胞（neuronoid stem cells derived from bone marrow stem cells，NdSCs-D—BMSCs）在脑室移植途径下对帕金森病（Parkinson disease，PD）大鼠的治疗作用。方法将酶切鉴定后的新构建质粒pEGFP—C2-TH经电穿孔法转染培养第8天NdSCs—D—BMSCs，注射到PD大鼠模型右侧脑室，观察大鼠行为学变化，移植细胞在大鼠脑组织内的迁移，以及高效液相方法检测脑内DA含量。结果质粒pEGFP—C2-TH转染NdSCs—D—BMSCs移植后10周，PD大鼠症状显著改善，DA恢复至正常水平33．0％，移植细胞可以在PD大鼠脑内存活，并出现远处迁移。结论TH修饰的大鼠NdSCs—D—BMSCs经脑室移植对PD大鼠具有明显的治疗作用，为临床中腰椎穿刺干细胞移植的应用提供实验依据。
基金the Excellent Discipline Leadership Fund Project of Harbin Science-Technology Administration :2006RFXYS044
文摘Objective To probe into the function mechanism of penetration therapy with head electrical acupuncture on Parkinson＇s disease. Methods Microinjection of 6-hydroxydopamin （6-OHDA） on the left cor- pus striatum was adopted to prepare rotation model of Parkinson^s disease in rat. Penetration therapy with head electrical acupuncture was administered in treatment. Normal group, sham-operation group, model group and penetration therapy group were set up. （1）lmmunohistochemical （IHC） method was used to test the morphology and count of positive cell of tyrosine hydroxylase （TH）. （2）RT-PCR technology was used to detect the expression of nestin mRNA of neural stem cell （NSC）. Results （1）Compared with model group, in pene- tration therapy group, the expressions of TH-positive neurons in immune response were increased in areal density （AD）, numerical density （ND） and integrating optic density （P〈0.05）. （2）Compared with model group, in penetration therapy group, the expression of nestin mRNA was increased （P〈0. 05）. Conclusion Penetration therapy with head electrical acupuncture promotes the proliferation of endogenous neural stem cells in substantia nigra of rat model of Parkinson＇s disease.
文摘Neural stem cell is presently the research hotspot in neuroscience. Recent progress indicates that epigenetic modulation is closely related to the self-renewal and differentiation of neural stem cell. Epigenetics refer to the study of mitotical/meiotical heritage changes in gene function that cannot be explained by changes in the DNA sequence. Major epigenetic mechanisms include DNA methylation, histone modification, chromatin remodeling, genomic imprinting, and non-coding RNA. In this review, we focus on the new insights into the epigenetic mechanism for neural stem cells fate.
基金We thank for the funding support from the National Natural Science Foundation of China(8197151612)Henan Provincial Project of Outstanding Talents in Science and Technology Innovation(2018JR0006).
文摘Stroke has been a common problem in the global public health because of its high incidence,recur-rence,disability rate and mortality,among which ischemic stroke accounts for about 80%of the total stroke.Cerebral ischemia reperfusion injury(CIRI)is one of the most important pathological injuries after ischemic stroke,and the effective treatment has always been a research focus at home and abroad.The proposal of the concept of neurovascular unit(NVU)which extends treatment ideas from local to holistic is in accordance with the holistic concept and treatment based on syndrome differentiation in Chinese medicine.Chinese medicine has many advantages,such as being multicomponent,multitarget,and multipath while stem cells are effective biological therapeutic means,both of which have been proved to exert comprehensive and definite effects on NVU injury caused by CIRI.This artide reviews the molecular mechanisms of NVU injury and the cerebropro-tective effects of Chinese medicine monomers or compound formulas combined with MSCs on NUV injury after cerebral ischemia,with a view to provide a theoretical basis for future studies.
基金Supported by the National Natural Sciences Foundation of China(No.81470171)Basic Science Research Fund from Ministry of Finance of China(No.ZZ2012008 and ZZ2015014)
文摘OBJECTIVE: To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS: Velvet antler(Cervus Nippon Temminck) polypeptide(VAP) was used to differentiate neural stem cells(NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immuno fl uorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.RESULTS: The differentiating cells look like neuron,some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.CONCLUSION: Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.
基金The work was supported by Natural Science Foundation of Guangdong Province （No.06028967, 8452402301001081 ）.
文摘Objective： To explore the electrophysiological proper- ties of differentiation of rat bone marrow-derived stromal stem cells （rBMSCs） to neuron-like cells in vitro by edaravone, a new type of free radical scavenger. Methods： Stromal stem cells were separated from rat bone marrow with Ficoll-Paque reagent and expanded in different culture medium in vitro, rBMSCs were induced by edaravone containing serum-free L-DMEM. Morphologic observation and Western blot analysis including the expression of Nav1.6, Kvl.2, Kv1.3, Cav1.2 were performed, and whole patch-clamp technique was used. Results： Cyton contraction and long processes were shown in differentiated stromal stem cells. Navl.6, Kvl.2, Kv 1.3 and Cav 1.2 were expressed in both differentiated and undifferentiated ceils. However, the expression of channel proteins in differentiated cells was up-regulated. Consistently, their resting potential and outward currents were also enhanced in the differentiated cells, which was especially significant in the outward rectifier potassium current. Conclusion： In vitro, neuron-like cells derived from rBMSCs, induced by edaravone, possess electrophysiological properties of neurons.
