Neuropeptide Y(NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica( SjNPYR-like) was ide...Neuropeptide Y(NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica( SjNPYR-like) was identified and characterized. The full-length SjNPYR-like cDNA was cloned containing a 492-bp of 5′ untranslated region(UTR), 1 182 bp open reading frame(ORF) encoding a protein of 393 amino acid residues, and 228 bp of 3′ UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point(pI) of 8.13. By informatic analyses, SjNPYR-like was identified as belonging to the class A G protein coupled receptor(GPCR) family(the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify SjNPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to Lymnaea stagnalis NPFR. Transmembrane properties of SjNPYR-like were demonstrated in vitro using HEK293 cells and the p EGFP-N1 plasmid. Relative quantifi cation of SjNPYR-like mRNA level confi rmed a high level expression and broad distribution of SjNPYR-like in various tissues of female S. japonica. In addition, the transcriptional profile of SjNPYR-like in the brain, liver, and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of SjNPYR-like and its potentially physical functions.展开更多
This paper investigates one aspect of the processing perspective in L2 （the second language） learning： the processing of new words. The article does not try to offer a comprehensive overview of language acquisition...This paper investigates one aspect of the processing perspective in L2 （the second language） learning： the processing of new words. The article does not try to offer a comprehensive overview of language acquisition mechanisms, but rather it intends to review more narrowly how our conceptual systems, governed by intricately linked networks of neural connections in the brain, make language development possible, at the same time creating some L2 processing problems. Vocabulary acquisition and application has been one of the main issues in English as a Second Language （ESL）/English as a Foreign Language （EFL） learning and teaching research. EFL instructors in Bosnia and Herzegovina, in particular, are coping with the effective vocabulary teaching approaches. The current paper will examine three principles based on cognitive linguistics （CL）, namely the study of categorization, prototype, and metaphor, and applications of these principles in formal vocabulary instruction in a Bosnian context of English learning in Bosnia and Herzegovina.展开更多
基金Supported by the Public Welfare Technical Applied Research Project of Zhejiang Province(No.2017C32074)the International Science&Technology Cooperation Program of China(No.2014DFT30120)the Open Foundation from Marine Sciences in the Most Important Subjects of Zhejiang(No.20130202)
文摘Neuropeptide Y(NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica( SjNPYR-like) was identified and characterized. The full-length SjNPYR-like cDNA was cloned containing a 492-bp of 5′ untranslated region(UTR), 1 182 bp open reading frame(ORF) encoding a protein of 393 amino acid residues, and 228 bp of 3′ UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point(pI) of 8.13. By informatic analyses, SjNPYR-like was identified as belonging to the class A G protein coupled receptor(GPCR) family(the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify SjNPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to Lymnaea stagnalis NPFR. Transmembrane properties of SjNPYR-like were demonstrated in vitro using HEK293 cells and the p EGFP-N1 plasmid. Relative quantifi cation of SjNPYR-like mRNA level confi rmed a high level expression and broad distribution of SjNPYR-like in various tissues of female S. japonica. In addition, the transcriptional profile of SjNPYR-like in the brain, liver, and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of SjNPYR-like and its potentially physical functions.
文摘This paper investigates one aspect of the processing perspective in L2 （the second language） learning： the processing of new words. The article does not try to offer a comprehensive overview of language acquisition mechanisms, but rather it intends to review more narrowly how our conceptual systems, governed by intricately linked networks of neural connections in the brain, make language development possible, at the same time creating some L2 processing problems. Vocabulary acquisition and application has been one of the main issues in English as a Second Language （ESL）/English as a Foreign Language （EFL） learning and teaching research. EFL instructors in Bosnia and Herzegovina, in particular, are coping with the effective vocabulary teaching approaches. The current paper will examine three principles based on cognitive linguistics （CL）, namely the study of categorization, prototype, and metaphor, and applications of these principles in formal vocabulary instruction in a Bosnian context of English learning in Bosnia and Herzegovina.
